SG00000001

SpermatogenesisOnline 1.0

Tag

Content

SG ID

SG00000001

UniProt Accession

D1MYR2_ANGJA;D1MYR2;

Theoretical PI

5.18

Molecular Weight

26455 Da

Genbank Nucleotide ID

AB519643;

Genbank Protein ID

BAI59110.1;

Gene Name

try

Gene Synonyms/Alias

Protein Name

Protein Synonyms/Alias

SubName: Trypsinogen

Organism

Anguilla japonica (Japanese eel)

NCBI Taxonomy ID

7937

Chromosome Location

chr:-;---;-

View in Ensembl genome browser

Function in Stage

postmeiotic

meiotic

premeiotic

Function in Cell Type

sertoli_cell

Description

Temporarily unavailable

The information of related literatures

1. C. Miura, T. Ohta, Y. Ozaki, H. Tanaka and T. Miura (2009) Trypsin is a multifunctional factor in spermatogenesis. Proc Natl Acad Sci U S A 106(49): 20972-7.

Abstract
Trypsin is well known as a pancreatic enzyme that is typically secreted into the intestine to digest proteins. We show in our current study, however, that trypsin is also a key factor in the control of spermatogenesis. A progestin in teleost fish, 17alpha, 20beta-dihydroxy-4-pregnen-3-one (DHP), is an essential component of the spermatogenesis pathway, particularly during the initiation of the first meiotic division. In the course of our investigations into the mechanisms underlying progestin-stimulated spermatogenesis, we identified that eel trypsinogen is upregulated in eel testis by DHP treatment. Trypsinogen is expressed in the Sertoli cells surrounding spermatogonia and in the membranes of spermatids and spermatozoa. Using an in vitro eel testicular culture system, we further analyzed the roles of trypsin in spermatogenesis. The inhibition of trypsin using specific antibodies or serine protease inhibitors was found to compromise DHP-induced spermatogenesis. A low dose of trypsin induces DNA synthesis and the expression of Spo11, a molecular marker of meiosis, in germ cells. By comparison, a higher dose of trypsin partially induced spermiogenesis. Furthermore, trypsin was detectable in the membranes of the spermatozoa and found to be associated with fertilization in fish. Our results thus demonstrate that trypsin and/or a trypsin-like protease is an essential and multifunctional factor in spermatogenesis. PMID: [19926858]

Figures for illustrating the function of this protein/gene

	Ref: C. Miura, T. Ohta, Y. Ozaki, H. Tanaka and T. Miura (2009) Trypsin is a multifunctional factor in spermatogenesis. Proc Natl Acad Sci U S A 106(49): 20972-7. PMID: [19926858]
	Ref: C. Miura, T. Ohta, Y. Ozaki, H. Tanaka and T. Miura (2009) Trypsin is a multifunctional factor in spermatogenesis. Proc Natl Acad Sci U S A 106(49): 20972-7. PMID: [19926858]
	Ref: C. Miura, T. Ohta, Y. Ozaki, H. Tanaka and T. Miura (2009) Trypsin is a multifunctional factor in spermatogenesis. Proc Natl Acad Sci U S A 106(49): 20972-7. PMID: [19926858]