Tag Content
SG ID
SG00000014 
UniProt Accession
Theoretical PI
8.95  
Molecular Weight
31499 Da  
Genbank Nucleotide ID
Genbank Protein ID
 
Gene Name
BT.44996 
Gene Synonyms/Alias
 
Protein Name
 
Protein Synonyms/Alias
SubName: Uncharacterized protein 
Organism
Bos taurus (Bovine) 
NCBI Taxonomy ID
9913 
Chromosome Location
chr:13;62106257-62151619;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. L. J. Bordeleau and P. Leclerc (2008) Expression of hck-tr, a truncated form of the src-related tyrosine kinase hck, in bovine spermatozoa and testis. Mol Reprod Dev 75(5): 828-37. 

Abstract
In bull testicular haploid germ cells, an mRNA encoding for hck was detected in addition to another one encoding for hck-tr, a truncated form of the tyrosine kinase hck. As the transcripts were expressed in spermatids, we tried to determine whether hck-tr is present in mature bovine spermatozoa. Two polyclonal antibodies were produced against peptides specific to the N- and C-terminal portions of the truncated protein. Western blot analyses confirmed the presence of hck-tr in total protein extracts of ejaculated bull spermatozoa, and sub-cellular fractionation experiments suggest its presence in both head and flagellum. The truncated protein appears tightly associated with cytoskeletal elements as it could be extracted only with SDS under reducing conditions. When assessed by indirect immunofluorescence, hck-tr was mostly localized at the acrosomal area of the sperm cell and a similar localization was observed on demembranated spermatozoa. Immunohistochemical studies on testis sections revealed protein expression in spermatocytes as well as in round and elongating spermatids. The results presented in this study clearly show the presence of mRNAs encoding for hck and hck-tr in testicular germ cells; hck-tr being translated during spermatogenesis and expressed on mature ejaculated bull spermatozoa. PMID: [17926339] 

Back to Top
Figures for illustrating the function of this protein/gene
Ref: L. J. Bordeleau and P. Leclerc (2008) Expression of hck-tr, a truncated form of the src-related tyrosine kinase hck, in bovine spermatozoa and testis. Mol Reprod Dev 75(5): 828-37. PMID: [17926339]
Ref: L. J. Bordeleau and P. Leclerc (2008) Expression of hck-tr, a truncated form of the src-related tyrosine kinase hck, in bovine spermatozoa and testis. Mol Reprod Dev 75(5): 828-37. PMID: [17926339]
Function
 
Back to Top
Subcellular Location
 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0004713 F:protein tyrosine kinase activity IEA:UniProtKB-KW.
Back to Top
Interpro
IPR011009;    Kinase-like_dom.
IPR000719;    Prot_kinase_cat_dom.
IPR017441;    Protein_kinase_ATP_BS.
IPR001245;    Ser-Thr/Tyr_kinase_cat_dom.
IPR000980;    SH2.
IPR001452;    SH3_domain.
Back to Top
Pfam
PF07714;    Pkinase_Tyr;    1.
PF00017;    SH2;    1.
Back to Top
SMART
SM00252;    SH2;    1.
Back to Top
PROSITE
PS00107;    PROTEIN_KINASE_ATP;    1.
PS50011;    PROTEIN_KINASE_DOM;    1.
PS50001;    SH2;    1.
PS50002;    SH3;    1.
Back to Top
PRINTS
PR00401;    SH2DOMAIN.;   
Back to Top
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
Nucleotide Sequence
Length: bp   Go to nucleotide: FASTA
Protein Sequence
Length: 276 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
Comments