SG00000017

SpermatogenesisOnline 1.0

Tag

Content

SG ID

SG00000017

UniProt Accession

CMGA_BOVIN;P05059;P79392;Q2KJ52;

Theoretical PI

12.18

Molecular Weight

50015 Da

Genbank Nucleotide ID

S79270; S79256; S79258; S79260; S79262; S79264; S79266; S79268; X04012; X04298; M16971; U73523; BC105515;

Genbank Protein ID

AAB21297.1; AAB21297.1; AAB21297.1; AAB21297.1; AAB21297.1; AAB21297.1; AAB21297.1; AAB21297.1; CAA27636.1; CAA27841.1; AAA30765.1; AAC48700.1; AAI05516.1;

Gene Name

CHGA

Gene Synonyms/Alias

Protein Name

Chromogranin-A Vasostatin-1 Chromostatin Chromacin Pancreastatin WE-14 Catestatin

Protein Synonyms/Alias

CgA Pituitary secretory protein I;SP-IContains:Contains:Contains:Contains:Contains:Contains:Flags: Precursor

Organism

Bos taurus (Bovine)

NCBI Taxonomy ID

9913

Chromosome Location

chr:21;58163028-58176319;1

View in Ensembl genome browser

Function in Stage

Function in Cell Type

Description

Temporarily unavailable

The information of related literatures

1. R. Payan-Carreira, P. Rodrigues and P. R. Carvalho (2006) Chromogranin-A expression in the bovine testis. Anim Reprod Sci 96(1-2): 146-53.

Abstract
Chromogranin-A (CgA) is the most distributed member of the granin family. Chromogranins are soluble anionic glycoproteins, found in the majority of the neuroendocrine and neural cells, co-stored with other endocrine substances (like insulin, glucagon, FSH and LH or NPY) in secretory granules. Outside the cell, it has been suggested that this peptide or one of its fragments, obtained by proteolytic cleavage, could act in an autocrine or paracrine way, regulating either the cell function or the contractibility of vascular segments. The purpose of the present study is to determine the distribution of chromogranin-A in the structures of the bovine testis. Immunohistochemical analysis was performed employing the biotin-streptavidin-peroxidase immunostaining technique in tissue specimens obtained at a local abbatoir. A CgA expression was found in the germinal epithelium at several stages of differentiation. Generally the strongest positive reaction was consistently observed in the basal compartment of the seminiferous tubules, with spermatogonia presenting a dense granular immunostaining pattern; a less intense reaction was also consistently recorded in type II spermatocytes and in round spermatids, which showed a more scattered disposition of CgA-positive granules. Clusters of Leydig cells also displayed a faint and homogeneous cytoplasmatic immunoreactivity for chromogranin-A. These results demonstrate a widely distribution of CgA-positive cells in the organism, and its presence in the testis raises the possibility of its participation in the cohort of local factors involved in the regulation of spermatogenesis. PMID: [16442243]

Figures for illustrating the function of this protein/gene

Function

Vasostatin-1 has antibacterial activity against Gram-positive bacteria M.luteus, B.megaterium. Not active against Gram-positive bacteria B.cereus, B.subtilis, S.pyogenes, M.fortuitum,S.aureus and L.monocytogenes and against Gram-negative bacteriaE.coli, E.cloacae, S.typhimurium, K.pneumoniae and P.aeruginosa.Possesses antifungal activity against N.crassa, A.fumigatus,A.brassicicola, N.hematococca, F.culmorum and F.oxyporum andagainst the yeast S.cerevisiae and C.albicans. Inactive againstT.mentagrophytes.

Subcellular Location

Cytoplasmic vesicle, secretory vesicle lumen(By similarity). Cytoplasmic vesicle, secretory vesicle membrane(By similarity). Secreted. Note=Associated with the secretorygranule membrane through direct interaction to SCG3 that in turnbinds to cholesterol-enriched lipid rafts in intragranularconditions (By similarity).

Tissue Specificity

Gene Ontology

GO ID	GO term	Evidence
GO:0005576	C:extracellular region	IDA:UniProtKB.
GO:0030141	C:secretory granule	IDA:UniProtKB.
GO:0030658	C:transport vesicle membrane	IEA:UniProtKB-SubCell.
GO:0050832	P:defense response to fungus	IDA:UniProtKB.
GO:0050829	P:defense response to Gram-negative bacterium	IDA:UniProtKB.
GO:0050830	P:defense response to Gram-positive bacterium	IDA:UniProtKB.
GO:0045087	P:innate immune response	NAS:UniProtKB.
GO:0031640	P:killing of cells of other organism	IEA:UniProtKB-KW.
GO:0016525	P:negative regulation of angiogenesis	TAS:UniProtKB.
GO:0046676	P:negative regulation of insulin secretion	IDA:UniProtKB.
GO:0030155	P:regulation of cell adhesion	TAS:UniProtKB.

Interpro

IPR001819;    Chromogranin_AB.
IPR018054;    Chromogranin_CS.
IPR001990;    Granin.
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Pfam

PF01271; Granin; 2.
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SMART

PROSITE

PS00422; GRANINS_1; 1.
PS00423; GRANINS_2; 1.
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PRINTS

PR00659; CHROMOGRANIN.;
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Created Date

18-Oct-2012

Record Type

Experiment identified

Protein sequence Annotation

SIGNAL        1     18
CHAIN        19    449       Chromogranin-A.
                             /FTId=PRO_0000005398.
PEPTIDE      19     94       Vasostatin-1.
                             /FTId=PRO_0000005399.
PEPTIDE     142    161       Chromostatin.
                             /FTId=PRO_0000005400.
PEPTIDE     191    212       Chromacin.
                             /FTId=PRO_0000005401.
PEPTIDE     266    312       Pancreastatin.
                             /FTId=PRO_0000005402.
PEPTIDE     334    347       WE-14.
                             /FTId=PRO_0000005403.
PEPTIDE     362    382       Catestatin.
                             /FTId=PRO_0000005404.
MOD_RES      99     99       Phosphoserine.
MOD_RES     142    142       Phosphoserine.
MOD_RES     191    191       Phosphotyrosine.
MOD_RES     200    200       Phosphoserine (By similarity).
MOD_RES     295    295       Phosphoserine (By similarity).
MOD_RES     312    312       Glycine amide (Probable).
MOD_RES     315    315       Phosphoserine.
MOD_RES     390    390       Phosphoserine.
MOD_RES     394    394       Phosphoserine.
CARBOHYD    185    185       O-linked (GalNAc...).
                             /FTId=CAR_000114.
CARBOHYD    204    204       O-linked (GalNAc...).
                             /FTId=CAR_000203.
CARBOHYD    249    249       O-linked (GalNAc...).
                             /FTId=CAR_000115.
DISULFID     35     56
CONFLICT    112    112       N -> T (in Ref. 4; AAA30765).
CONFLICT    136    136       F -> S (in Ref. 2; CAA27636 and 6;
                             AAI05516).
CONFLICT    154    155       SP -> PQ (in Ref. 3; CAA27841).
CONFLICT    159    159       P -> R (in Ref. 3; CAA27841).
CONFLICT    191    191       Y -> H (in Ref. 1; AAB21297).
CONFLICT    254    254       P -> A (in Ref. 1; AAB21297).
CONFLICT    293    293       A -> S (in Ref. 5; AAC48700).
CONFLICT    311    311       R -> H (in Ref. 2; CAA27636, 6; AAI05516
                             and 13; AA sequence).
CONFLICT    319    319       E -> K (in Ref. 2; CAA27636, 6; AAI05516
                             and 13; AA sequence).
CONFLICT    379    379       G -> R (in Ref. 4; AAA30765).
CONFLICT    391    391       R -> Q (in Ref. 2; CAA27636 and 6;
                             AAI05516).
TURN        369    374
STRAND      375    377
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Nucleotide Sequence

Length: 599 bp Go to nucleotide: FASTA

Protein Sequence

Length: 449 bp Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
STX1A_BOVIN	STX1A	MINT
SYT1_BOVIN	SYT1	MINT

Other Protein-Protein interaction resources

String database

View Microarray data

Temporarily unavailable

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