SG ID

SG00000034 

UniProt Accession

Q5FC64_CAEEL;Q5FC64;  

Theoretical PI

8.56  

Molecular Weight

41431 Da  

Genbank Nucleotide ID

Z71177;  

Genbank Protein ID

CAI46554.1; 

Gene Name

spe-10 

Gene Synonyms/Alias

ORFNames=AC3.10 

Protein Name

 

Protein Synonyms/Alias

SubName: Protein SPE-10 

Caenorhabditis elegans 

6239 

Temporarily unavailable 

The information of related literatures

1. E. J. Gleason, W. C. Lindsey, T. L. Kroft, A. W. Singson and W. L'Hernault S (2006) spe-10 encodes a DHHC-CRD zinc-finger membrane protein required for endoplasmic reticulum/Golgi membrane morphogenesis during Caenorhabditis elegans spermatogenesis. Genetics 172(1): 145-58. 

Abstract
C. elegans spermatogenesis employs lysosome-related fibrous body-membranous organelles (FB-MOs) for transport of many cellular components. Previous work showed that spe-10 mutants contain FB-MOs that prematurely disassemble, resulting in defective transport of FB components into developing spermatids. Consequently, spe-10 spermatids are smaller than wild type and contain defective FB-MO derivatives. In this article, we show that spe-10 encodes a four-pass integral membrane protein that has a DHHC-CRD zinc-finger motif. The DHHC-CRD motif is found in a large, diverse family of proteins that have been implicated in palmitoyl transfer during protein lipidation. Seven spe-10 mutants were analyzed, including missense, nonsense, and deletion mutants. An antiserum to SPE-10 showed significant colocalization with a known marker for the FB-MOs during wild-type spermatogenesis. In contrast, the spe-10(ok1149) deletion mutant lacked detectable SPE-10 staining; this mutant lacks a spe-10 promoter and most coding sequence. The spe-10(eb64) missense mutation, which changes a conserved residue within the DHHC-CRD domain in all homologues, behaves as a null mutant. These results suggest that wild-type SPE-10 is required for the MO to properly deliver the FB to the C. elegans spermatid and the DHHC-CRD domain is essential for this function. PMID: [16143610] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0043227	C:membrane-bounded organelle	IDA:WormBase.
GO:0008270	F:zinc ion binding	IEA:InterPro.
GO:0006944	P:cellular membrane fusion	IMP:WormBase.
GO:0040023	P:establishment of nucleus localization	IMP:WormBase.
GO:0006996	P:organelle organization	IMP:WormBase.
GO:0045793	P:positive regulation of cell size	IMP:WormBase.
GO:0031268	P:pseudopodium organization	IMP:WormBase.
GO:0030317	P:sperm motility	IMP:WormBase.
GO:0007286	P:spermatid development	IMP:WormBase.

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Interpro

IPR001594;    Znf_DHHC_palmitoyltrfase.
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Pfam

PF01529;    zf-DHHC;    1.
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SMART

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PROSITE

PS50216;    ZF_DHHC;    1.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 38951 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 351 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

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Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

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View Microarray data

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