SG ID

SG00000038 

UniProt Accession

ASF1L_CAEEL;Q17603;  

Theoretical PI

4.13  

Molecular Weight

27997 Da  

Genbank Nucleotide ID

Z75525;  

Genbank Protein ID

CAA99762.3; 

Gene Name

asfl-1 

Gene Synonyms/Alias

ORFNames=C03D6.5 

Protein Name

Probable histone chaperone asf-1-like protein 

Protein Synonyms/Alias

Anti-silencing function protein 1-like; 

Caenorhabditis elegans 

6239 

Temporarily unavailable 

The information of related literatures

1. I. F. Grigsby, E. M. Rutledge, C. A. Morton and F. P. Finger (2009) Functional redundancy of two C. elegans homologs of the histone chaperone Asf1 in germline DNA replication. Dev Biol 329(1): 64-79. 

Abstract
Eukaryotic genomes contain either one or two genes encoding homologs of the highly conserved histone chaperone Asf1, however, little is known of their in vivo roles in animal development. UNC-85 is one of the two Caenorhabditis elegans Asf1 homologs and functions in post-embryonic replication in neuroblasts. Although UNC-85 is broadly expressed in replicating cells, the specificity of the mutant phenotype suggested possible redundancy with the second C. elegans Asf1 homolog, ASFL-1. The asfl-1 mRNA is expressed in the meiotic region of the germline, and mutants in either Asf1 genes have reduced brood sizes and low penetrance defects in gametogenesis. The asfl-1, unc-85 double mutants are sterile, displaying defects in oogenesis and spermatogenesis, and analysis of DNA synthesis revealed that DNA replication in the germline is blocked. Analysis of somatic phenotypes previously observed in unc-85 mutants revealed that they are neither observed in asfl-1 mutants, nor enhanced in the double mutants, with the exception of enhanced male tail abnormalities in the double mutants. These results suggest that the two Asf1 homologs have partially overlapping functions in the germline, while UNC-85 is primarily responsible for several Asf1 functions in somatic cells, and is more generally involved in replication throughout development. PMID: [19233156] 

Figures for illustrating the function of this protein/gene

Function

Histone chaperone that facilitates histone depositionand histone exchange and removal during nucleosome assembly anddisassembly (By similarity). 

Subcellular Location

Nucleus (By similarity). 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005634	C:nucleus	IC:UniProtKB.
GO:0006333	P:chromatin assembly or disassembly	IEA:InterPro.
GO:0016568	P:chromatin modification	IEA:UniProtKB-KW.
GO:0006260	P:DNA replication	IMP:UniProtKB.
GO:0048477	P:oogenesis	IMP:UniProtKB.
GO:0060378	P:regulation of brood size	IMP:UniProtKB.
GO:0006355	P:regulation of transcription, DNA-dependent	IEA:UniProtKB-KW.
GO:0007283	P:spermatogenesis	IMP:UniProtKB.
GO:0006351	P:transcription, DNA-dependent	IEA:UniProtKB-KW.

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Interpro

IPR017282;    Hist_deposition_Asf1.
IPR006818;    Histone_chaperone_ASF1-like.
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Pfam

PF04729;    ASF1_hist_chap;    1.
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SMART

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PROSITE

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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 15509 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 245 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

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View Microarray data

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