SG00000048

SpermatogenesisOnline 1.0

Tag

Content

SG ID

SG00000048

UniProt Accession

G5EBQ3_CAEEL;G5EBQ3;

Theoretical PI

8.52

Molecular Weight

190057 Da

Genbank Nucleotide ID

AF159143; Z78419;

Genbank Protein ID

AAF80367.1; CAB01701.2;

Gene Name

ego-1

Gene Synonyms/Alias

Protein Name

Protein Synonyms/Alias

SubName: Protein EGO-1SubName: RNA-directed RNA polymerase related EGO-1

Organism

Caenorhabditis elegans

NCBI Taxonomy ID

6239

Chromosome Location

chr:I;7650410-7656332;-1

View in Ensembl genome browser

Function in Stage

postmeiotic

meiotic

Function in Cell Type

spermatocyte

spermatid

Description

Temporarily unavailable

The information of related literatures

1. E. M. Maine, J. Hauth, T. Ratliff, V. E. Vought, X. She and W. G. Kelly (2005) EGO-1, a putative RNA-dependent RNA polymerase, is required for heterochromatin assembly on unpaired dna during C. elegans meiosis. Curr Biol 15(21): 1972-8.

Abstract
During meiosis in C. elegans, unpaired chromosomes and chromosomal regions accumulate high levels of histone H3 lysine 9 dimethylation (H3K9me2), a modification associated with facultative heterochromatin assembly and the resulting transcriptional silencing. Meiotic silencing of unpaired DNA may be a widely conserved genome defense mechanism. The mechanisms of meiotic silencing remain unclear, although both transcriptional and posttranscriptional processes are implicated. Cellular RNA-dependent RNA polymerases (RdRPs) function in development and RNA-mediated silencing in many species and in heterochromatin assembly in S. pombe. There are four C. elegans RdRPs, including two with known germline functions. EGO-1 is required for fertility and robust germline RNAi. RRF-3 acts genetically to repress RNAi and is required for normal meiosis and spermatogenesis at elevated temperatures (S. L'Hernault, personal communication). Among C. elegans RdRPs, we find that only EGO-1 is required for H3K9me2 enrichment on unpaired chromosomal regions during meiosis. This H3K9me2 enrichment does not require Dicer or Drosha nuclease or any of several other proteins required for RNAi. ego-1 interacts genetically with him-17, another regulator of chromatin and meiosis, to promote germline development. We conclude that EGO-1 is an essential component of meiotic silencing in C. elegans. PMID: [16271877]

2. A. Smardon, J. M. Spoerke, S. C. Stacey, M. E. Klein, N. Mackin and E. M. Maine (2000) EGO-1 is related to RNA-directed RNA polymerase and functions in germ-line development and RNA interference in C. elegans. Curr Biol 10(4): 169-78.

Abstract
BACKGROUND PMID: [10704412]

Figures for illustrating the function of this protein/gene

	Ref: E. M. Maine, J. Hauth, T. Ratliff, V. E. Vought, X. She and W. G. Kelly (2005) EGO-1, a putative RNA-dependent RNA polymerase, is required for heterochromatin assembly on unpaired dna during C. elegans meiosis. Curr Biol 15(21): 1972-8. PMID: [16271877]
	Ref: E. M. Maine, J. Hauth, T. Ratliff, V. E. Vought, X. She and W. G. Kelly (2005) EGO-1, a putative RNA-dependent RNA polymerase, is required for heterochromatin assembly on unpaired dna during C. elegans meiosis. Curr Biol 15(21): 1972-8. PMID: [16271877]
	Ref: E. M. Maine, J. Hauth, T. Ratliff, V. E. Vought, X. She and W. G. Kelly (2005) EGO-1, a putative RNA-dependent RNA polymerase, is required for heterochromatin assembly on unpaired dna during C. elegans meiosis. Curr Biol 15(21): 1972-8. PMID: [16271877]
	Ref: A. Smardon, J. M. Spoerke, S. C. Stacey, M. E. Klein, N. Mackin and E. M. Maine (2000) EGO-1 is related to RNA-directed RNA polymerase and functions in germ-line development and RNA interference in C. elegans. Curr Biol 10(4): 169-78. PMID: [10704412]