SG ID

SG00000055 

UniProt Accession

ZYG1_CAEEL;Q9GT24;O01903;  

Theoretical PI

9.71  

Molecular Weight

79415 Da  

Genbank Nucleotide ID

AF285179; FO080594;  

Genbank Protein ID

AAG15377.1; CCD64966.1; 

Gene Name

zyg-1 

Gene Synonyms/Alias

ORFNames=F59E12.2 

Protein Name

Probable serine/threonine-protein kinase zyg-1 

Protein Synonyms/Alias

EC=2.7.11.1 Zygote defective protein 1; 

Caenorhabditis elegans 

6239 

Temporarily unavailable 

The information of related literatures

1. N. Peters, D. E. Perez, M. H. Song, Y. Liu, T. Muller-Reichert, C. Caron, K. J. Kemphues and K. F. O'Connell (2010) Control of mitotic and meiotic centriole duplication by the Plk4-related kinase ZYG-1. J Cell Sci 123(Pt 5): 795-805. 

Abstract
Centriole duplication is of crucial importance during both mitotic and male meiotic divisions, but it is currently not known whether this process is regulated differently during the two modes of division. In Caenorhabditis elegans, the kinase ZYG-1 plays an essential role in both mitotic and meiotic centriole duplication. We have found that the C-terminus of ZYG-1 is necessary and sufficient for targeting to centrosomes and is important for differentiating mitotic and meiotic centriole duplication. Small truncations of the C-terminus dramatically lower the level of ZYG-1 at mitotic centrosomes but have little effect on the level of ZYG-1 at meiotic centrosomes. Interestingly, truncation of ZYG-1 blocks centrosome duplication in the mitotic cycle but leads to centrosome amplification in the meiotic cycle. Meiotic centriole amplification appears to result from the overduplication of centrioles during meiosis I and leads to the formation of multipolar meiosis II spindles. The extra centrioles also disrupt spermatogenesis by inducing the formation of supernumerary fertilization-competent spermatids that contain abnormal numbers of chromosomes and centrioles. Our data reveal differences in the regulation of mitotic and meiotic centrosome duplication, particularly with regard to ZYG-1 activity, and reveal an important role for centrosomes in spermatid formation. PMID: [20144993] 

Figures for illustrating the function of this protein/gene

Function

Protein kinase that plays a central role in centrosomeduplication. Paternal copy is required to regulate synthesis ofdaughter centrioles prior to fertilization. Maternal copyregulates centrosome duplication during later cell cycles.Functions upstream of sas-5 and sas-6, and is required for theirlocalization to the centrosome. 

Subcellular Location

Cytoplasm, cytoskeleton, centrosome.Cytoplasm, cytoskeleton, centrosome, centriole. Note=Component ofthe centrosome. Present at centrioles only immediately beforetheir duplication. 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005814	C:centriole	IDA:WormBase.
GO:0005524	F:ATP binding	IEA:UniProtKB-KW.
GO:0004672	F:protein kinase activity	IDA:WormBase.
GO:0004674	F:protein serine/threonine kinase activity	IEA:UniProtKB-KW.
GO:0004713	F:protein tyrosine kinase activity	IEA:InterPro.
GO:0051301	P:cell division	IMP:WormBase.
GO:0007099	P:centriole replication	IMP:WormBase.
GO:0009792	P:embryo development ending in birth or egg hatching	IMP:WormBase.
GO:0040011	P:locomotion	IMP:WormBase.
GO:0008104	P:protein localization	IMP:WormBase.
GO:0000003	P:reproduction	IMP:WormBase.
GO:0051225	P:spindle assembly	IMP:WormBase.

Back to Top

Interpro

IPR011009;    Kinase-like_dom.
IPR000719;    Prot_kinase_cat_dom.
IPR017441;    Protein_kinase_ATP_BS.
IPR008266;    Tyr_kinase_AS.
Back to Top

Pfam

PF00069;    Pkinase;    1.
Back to Top

SMART

Back to Top

PROSITE

PS00107;    PROTEIN_KINASE_ATP;    1.
PS50011;    PROTEIN_KINASE_DOM;    1.
PS00109;    PROTEIN_KINASE_TYR;    1.
Back to Top

PRINTS

Back to Top

Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 2364 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 706 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
ATPA_CAEEL	H28O16.1	IntAct
PAL1_CAEEL	pal-1	IntAct
Q17695_CAEEL	spdl-1	IntAct
Q18282_CAEEL	mesp-1	IntAct
Q18955_CAEEL	unc-120	IntAct
Q20183_CAEEL	tag-30	IntAct
Q22847_CAEEL	T28C6.7	IntAct
Q27864	nex-1	IntAct
Q9GRZ9_CAEEL	Y59A8A.3	IntAct
Q9U302_CAEEL	pab-1	IntAct
Q9XU77	uso-1	DIP
Q9XWJ0	tac-1	DIP
RL3_CAEEL	rpl-3	IntAct
TPM1_CAEEL	lev-11	IntAct
ATPA_CAEEL	H28O16.1	BioGRID
CLH_CAEEL	chc-1	IntAct
PAL1_CAEEL	pal-1	BioGRID
Q17695_CAEEL	spdl-1	MINT
Q18282_CAEEL	mesp-1	MINT
Q18955_CAEEL	unc-120	BioGRID
Q20183_CAEEL	tag-30	MINT
Q22847_CAEEL	T28C6.7	MINT
Q22866-2	lev-11	MINT
Q27864	nex-1	BioGRID
Q6BEU5	rpl-3	BioGRID
Q6BEU6	rpl-3	BioGRID
Q7K797_CAEEL	pab-1	BioGRID
Q7K798_CAEEL	pab-1	BioGRID
Q9GRZ9_CAEEL	Y59A8A.3	MINT
Q9U302_CAEEL	pab-1	BioGRID
Q9XU77	uso-1	IntAct
Q9XWJ0	tac-1	IntAct
RL3_CAEEL	rpl-3	BioGRID

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

Comments