Tag Content
SG ID
SG00000062 
UniProt Accession
Theoretical PI
5.27  
Molecular Weight
120641 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
glh-4 
Gene Synonyms/Alias
ORFNames=T12F5.3 
Protein Name
ATP-dependent RNA helicase glh-4 
Protein Synonyms/Alias
EC=3.6.4.13 Germline helicase 4; 
Organism
Caenorhabditis elegans 
NCBI Taxonomy ID
6239 
Chromosome Location
chr:I;3726014-3731227;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. K. A. Kuznicki, P. A. Smith, W. M. Leung-Chiu, A. O. Estevez, H. C. Scott and K. L. Bennett (2000) Combinatorial RNA interference indicates GLH-4 can compensate for GLH-1; these two P granule components are critical for fertility in C. elegans. Development 127(13): 2907-16. 

Abstract
We report that four putative germline RNA helicases, GLHs, are components of the germline-specific P granules in Caenorhabditis elegans. GLH-3 and GLH-4, newly discovered, belong to a multi-gene glh family. Although GLHs are homologous to Drosophila VASA, a polar granule component necessary for oogenesis and embryonic pattern formation, the GLHs are distinguished by containing multiple CCHC zinc fingers. RNA-mediated interference (RNAi) reveals the GLHs are critical for oogenesis. By RNAi at 20 degrees C, when either loss of GLH-1 or GLH-4 alone has no effect, loss of both GLH-1 and GLH-4 results in 97% sterility in the glh-1/4(RNAi) offspring of injected hermaphrodites. glh-1/4(RNAi) germlines are under-proliferated and are without oocytes. glh-1/4(RNAi) animals produce sperm; however, spermatogenesis is delayed and the sperm are defective. P granules are still present in glh-1/4(RNAi) sterile worms as revealed with antibodies against the remaining GLH-2 and GLH-3 proteins, indicating the GLHs function independently in P granule assembly. These studies reveal that C.elegans can use GLH-1 or GLH-4 to promote germline development. PMID: [10851135] 

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Figures for illustrating the function of this protein/gene
Ref: K. A. Kuznicki, P. A. Smith, W. M. Leung-Chiu, A. O. Estevez, H. C. Scott and K. L. Bennett (2000) Combinatorial RNA interference indicates GLH-4 can compensate for GLH-1; these two P granule components are critical for fertility in C. elegans. Development 127(13): 2907-16. PMID: [10851135]
Ref: K. A. Kuznicki, P. A. Smith, W. M. Leung-Chiu, A. O. Estevez, H. C. Scott and K. L. Bennett (2000) Combinatorial RNA interference indicates GLH-4 can compensate for GLH-1; these two P granule components are critical for fertility in C. elegans. Development 127(13): 2907-16. PMID: [10851135]
Ref: K. A. Kuznicki, P. A. Smith, W. M. Leung-Chiu, A. O. Estevez, H. C. Scott and K. L. Bennett (2000) Combinatorial RNA interference indicates GLH-4 can compensate for GLH-1; these two P granule components are critical for fertility in C. elegans. Development 127(13): 2907-16. PMID: [10851135]
Ref: K. A. Kuznicki, P. A. Smith, W. M. Leung-Chiu, A. O. Estevez, H. C. Scott and K. L. Bennett (2000) Combinatorial RNA interference indicates GLH-4 can compensate for GLH-1; these two P granule components are critical for fertility in C. elegans. Development 127(13): 2907-16. PMID: [10851135]
Function
Probable ATP-binding RNA helicase. 
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Subcellular Location
 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0043186 C:P granule IDA:WormBase.
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0008026 F:ATP-dependent helicase activity IEA:InterPro.
GO:0003723 F:RNA binding IEA:UniProtKB-KW.
GO:0003724 F:RNA helicase activity ISS:WormBase.
GO:0008270 F:zinc ion binding IEA:InterPro.
GO:0007276 P:gamete generation IMP:WormBase.
GO:0042127 P:regulation of cell proliferation IMP:WormBase.
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Interpro
IPR011545;    DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001;    Helicase_ATP-bd.
IPR001650;    Helicase_C.
IPR000629;    RNA-helicase_DEAD-box_CS.
IPR014014;    RNA_helicase_DEAD_Q_motif.
IPR001878;    Znf_CCHC.
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Pfam
PF00270;    DEAD;    1.
PF00271;    Helicase_C;    1.
PF00098;    zf-CCHC;    4.
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SMART
SM00487;    DEXDc;    1.
SM00490;    HELICc;    1.
SM00343;    ZnF_C2HC;    5.
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PROSITE
PS00039;    DEAD_ATP_HELICASE;    1.
PS51192;    HELICASE_ATP_BIND_1;    1.
PS51194;    HELICASE_CTER;    1.
PS51195;    Q_MOTIF;    1.
PS50158;    ZF_CCHC;    5.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   1156       ATP-dependent RNA helicase glh-4.
                             /FTId=PRO_0000055092.
DOMAIN      767    951       Helicase ATP-binding.
DOMAIN      986   1139       Helicase C-terminal.
ZN_FING     570    587       CCHC-type 1.
ZN_FING     593    610       CCHC-type 2.
ZN_FING     616    633       CCHC-type 3.
ZN_FING     639    656       CCHC-type 4.
ZN_FING     665    682       CCHC-type 5.
NP_BIND     780    787       ATP (By similarity).
MOTIF       736    764       Q motif.
MOTIF       897    900       DEAD box.
CONFLICT    136    136       L -> P (in Ref. 1; AAC28387).
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Nucleotide Sequence
Length: 3848 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 1156 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
kgb-1IntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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