SG ID

SG00000063 

UniProt Accession

GLH1_CAEEL;P34689;Q22873;Q7KQH5;Q9TXH4;  

Theoretical PI

5.55  

Molecular Weight

79780 Da  

Genbank Nucleotide ID

L19948; U62772; FO080897;  

Genbank Protein ID

AAC27384.1; AAB04136.1; CCD67591.1; 

Gene Name

glh-1 

Gene Synonyms/Alias

ORFNames=T21G5.3 

Protein Name

ATP-dependent RNA helicase glh-1 

Protein Synonyms/Alias

EC=3.6.4.13 Germline helicase 1; 

Caenorhabditis elegans 

6239 

Temporarily unavailable 

The information of related literatures

1. K. A. Kuznicki, P. A. Smith, W. M. Leung-Chiu, A. O. Estevez, H. C. Scott and K. L. Bennett (2000) Combinatorial RNA interference indicates GLH-4 can compensate for GLH-1; these two P granule components are critical for fertility in C. elegans. Development 127(13): 2907-16. 

Abstract
We report that four putative germline RNA helicases, GLHs, are components of the germline-specific P granules in Caenorhabditis elegans. GLH-3 and GLH-4, newly discovered, belong to a multi-gene glh family. Although GLHs are homologous to Drosophila VASA, a polar granule component necessary for oogenesis and embryonic pattern formation, the GLHs are distinguished by containing multiple CCHC zinc fingers. RNA-mediated interference (RNAi) reveals the GLHs are critical for oogenesis. By RNAi at 20 degrees C, when either loss of GLH-1 or GLH-4 alone has no effect, loss of both GLH-1 and GLH-4 results in 97% sterility in the glh-1/4(RNAi) offspring of injected hermaphrodites. glh-1/4(RNAi) germlines are under-proliferated and are without oocytes. glh-1/4(RNAi) animals produce sperm; however, spermatogenesis is delayed and the sperm are defective. P granules are still present in glh-1/4(RNAi) sterile worms as revealed with antibodies against the remaining GLH-2 and GLH-3 proteins, indicating the GLHs function independently in P granule assembly. These studies reveal that C.elegans can use GLH-1 or GLH-4 to promote germline development. PMID: [10851135] 

Figures for illustrating the function of this protein/gene

Function

Probable ATP-binding RNA helicase. 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0043186	C:P granule	IDA:WormBase.
GO:0005524	F:ATP binding	IEA:UniProtKB-KW.
GO:0008026	F:ATP-dependent helicase activity	IEA:InterPro.
GO:0003723	F:RNA binding	IEA:UniProtKB-KW.
GO:0003724	F:RNA helicase activity	IDA:WormBase.
GO:0008270	F:zinc ion binding	IEA:InterPro.
GO:0007281	P:germ cell development	IMP:WormBase.
GO:0009791	P:post-embryonic development	IMP:WormBase.
GO:0016070	P:RNA metabolic process	ISS:WormBase.

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Interpro

IPR011545;    DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001;    Helicase_ATP-bd.
IPR001650;    Helicase_C.
IPR000629;    RNA-helicase_DEAD-box_CS.
IPR014014;    RNA_helicase_DEAD_Q_motif.
IPR001878;    Znf_CCHC.
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Pfam

PF00270;    DEAD;    1.
PF00271;    Helicase_C;    1.
PF00098;    zf-CCHC;    4.
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SMART

SM00487;    DEXDc;    1.
SM00490;    HELICc;    1.
SM00343;    ZnF_C2HC;    4.
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PROSITE

PS00039;    DEAD_ATP_HELICASE;    1.
PS51192;    HELICASE_ATP_BIND_1;    1.
PS51194;    HELICASE_CTER;    1.
PS51195;    Q_MOTIF;    1.
PS50158;    ZF_CCHC;    4.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 2436 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 763 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
BAR1_CAEEL	bar-1	BioGRID
EGL15_CAEEL	egl-15	BioGRID
CSN5_CAEEL	csn-5	IntAct
GLH1_CAEEL	glh-1	IntAct
GLH1_CAEEL	glh-1	IntAct
GLH3_CAEEL	glh-3	IntAct
O44408_CAEEL	kgb-1	IntAct
GLH3_CAEEL	glh-3	IntAct
LE756_CAEEL	let-756	BioGRID
LET60_CAEEL	let-60	BioGRID
Q9U3F4_CAEEL	zyx-1	IntAct

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

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