SG ID

SG00000078 

UniProt Accession

SPE4_CAEEL;Q01608;  

Theoretical PI

4.45  

Molecular Weight

51830 Da  

Genbank Nucleotide ID

Z14066; Z14067; Z73912;  

Genbank Protein ID

CAA78449.1; CAA78450.1; CAA98145.1; 

Gene Name

spe-4 

Gene Synonyms/Alias

ORFNames=ZK524.1 

Protein Name

Presenilin spe-4 

Protein Synonyms/Alias

 

Caenorhabditis elegans 

6239 

Temporarily unavailable 

The information of related literatures

1. R. Gosney, W. S. Liau and C. W. Lamunyon (2008) A novel function for the presenilin family member spe-4. BMC Dev Biol 8(): 44. 

Abstract
BACKGROUND PMID: [18430247] 

2. S. W. L'Hernault and P. M. Arduengo (1992) Mutation of a putative sperm membrane protein in Caenorhabditis elegans prevents sperm differentiation but not its associated meiotic divisions. J Cell Biol 119(1): 55-68. 

Abstract
Spermatogenesis in the nematode Caenorhabditis elegans uses unusual organelles, called the fibrous body-membranous organelle (FB-MO) complexes, to prepackage and deliver macromolecules to spermatids during cytokinesis that accompanies the second meiotic division. Mutations in the spe-4 (spermatogenesis-defective) gene disrupt these organelles and prevent cytokinesis during spermatogenesis, but do not prevent completion of the meiotic nuclear divisions that normally accompany spermatid formation. We report an ultrastructural analysis of spe-4 mutant sperm where the normally close association of the FB's with the MO's and the double layered membrane surrounding the FB's are both defective. The internal membrane structure of the MO's is also disrupted in spe-4 mutant sperm. Although sperm morphogenesis in spe-4 mutants arrests prior to the formation of spermatids, meiosis can apparently be completed so that haploid nuclei reside in an arrested spermatocyte. We have cloned the spe-4 gene in order to understand its role during spermatogenesis and the molecular basis of how mutation of this gene disrupts this process. The spe-4 gene encodes an approximately 1.5-kb mRNA that is expressed during spermatogenesis, and the sequence of this gene suggests that it encodes an integral membrane protein. These data suggest that mutation of an integral membrane protein within FB-MO complexes disrupts morphogenesis and prevents formation of spermatids but does not affect completion of the meiotic nuclear divisions in C. elegans sperm. PMID: [1527173] 

3. P. M. Arduengo, O. K. Appleberry, P. Chuang and S. W. L'Hernault (1998) The presenilin protein family member SPE-4 localizes to an ER/Golgi derived organelle and is required for proper cytoplasmic partitioning during Caenorhabditis elegans spermatogenesis. J Cell Sci 111 ( Pt 24)(): 3645-54. 

Abstract
During Caenorhabditis elegans spermatogenesis, asymmetric partitioning of cellular components principally occurs via ER/Golgi-derived organelles, named fibrous body-membranous organelles. In C. elegans spe-4 mutants, morphogenesis of fibrous body-membranous organelle complexes is defective and spermatogenesis arrests at an unusual cellular stage with four haploid nuclei within a common cytoplasm. The spe-4 encoded integral membrane protein is a diverged member of the presenilin family implicated in early onset Alzheimer's disease. Specific antisera were used to show that SPE-4 resides within the fibrous body-membranous organelles membranes during wild-type spermatogenesis. Several spe-4 recessive mutants were examined for SPE-4 immunoreactivity and a deletion mutant lacks detectable SPE-4 while either of two missense mutants synthesize and localize immunoreactive SPE-4 within their fibrous body-membranous organelles. One of these missense mutations is located within a motif that is common to all presenilins. spe-4 mutants were also examined for other partitioning defects and tubulin was found to accumulate in unusual deposits close to the plasma membrane. These results suggest that wild-type SPE-4 is required for proper localization of macromolecules that are subject to asymmetric partitioning during spermatogenesis. PMID: [9819355] 

Figures for illustrating the function of this protein/gene

Function

Potential catalytic subunit of the gamma-secretasecomplex during spermatogenesis, an endoprotease complex thatcatalyzes the intramembrane cleavage of integral membrane proteinssuch as Notch receptors (lin-12 or glp-1). Involved in spermatidformation during meiosis II. May be required for properlocalization of macromolecules that are subject to asymmetricpartitioning during spermatogenesis. 

Subcellular Location

Endoplasmic reticulum membrane; Multi-passmembrane protein. Golgi apparatus, cis-Golgi network membrane;Multi-pass membrane protein. Note=Predominantly located in theendoplasmic reticulum and in the cis-Golgi. 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0016324	C:apical plasma membrane	IBA:RefGenome.
GO:0030424	C:axon	IBA:RefGenome.
GO:0005938	C:cell cortex	IBA:RefGenome.
GO:0009986	C:cell surface	IBA:RefGenome.
GO:0035253	C:ciliary rootlet	IBA:RefGenome.
GO:0043198	C:dendritic shaft	IBA:RefGenome.
GO:0005789	C:endoplasmic reticulum membrane	IEA:UniProtKB-SubCell.
GO:0005794	C:Golgi apparatus	IEA:UniProtKB-SubCell.
GO:0030426	C:growth cone	IBA:RefGenome.
GO:0005887	C:integral to plasma membrane	IBA:RefGenome.
GO:0005765	C:lysosomal membrane	IBA:RefGenome.
GO:0045121	C:membrane raft	IBA:RefGenome.
GO:0005743	C:mitochondrial inner membrane	IBA:RefGenome.
GO:0031594	C:neuromuscular junction	IBA:RefGenome.
GO:0043025	C:neuronal cell body	IBA:RefGenome.
GO:0005634	C:nucleus	IBA:RefGenome.
GO:0048471	C:perinuclear region of cytoplasm	IBA:RefGenome.
GO:0030018	C:Z disc	IBA:RefGenome.
GO:0004190	F:aspartic-type endopeptidase activity	IEA:InterPro.
GO:0004175	F:endopeptidase activity	IBA:RefGenome.
GO:0042987	P:amyloid precursor protein catabolic process	IBA:RefGenome.
GO:0006916	P:anti-apoptosis	IBA:RefGenome.
GO:0008105	P:asymmetric protein localization	IMP:WormBase.
GO:0050435	P:beta-amyloid metabolic process	IBA:RefGenome.
GO:0006816	P:calcium ion transport	IBA:RefGenome.
GO:0016044	P:cellular membrane organization	IMP:WormBase.
GO:0006509	P:membrane protein ectodomain proteolysis	IBA:RefGenome.
GO:0007220	P:Notch receptor processing	IBA:RefGenome.
GO:0007219	P:Notch signaling pathway	IEA:UniProtKB-KW.
GO:0006996	P:organelle organization	IMP:WormBase.
GO:0007286	P:spermatid development	IMP:WormBase.

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Interpro

IPR006639;    Peptidase_A22.
IPR001108;    Peptidase_A22A.
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Pfam

PF01080;    Presenilin;    1.
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SMART

SM00730;    PSN;    1.
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PROSITE

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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 1484 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 465 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

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Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

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View Microarray data

Temporarily unavailable 

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