SG ID

SG00000101 

UniProt Accession

E2FHQ0_CLAGA;E2FHQ0;  

Theoretical PI

6.32  

Molecular Weight

50997 Da  

Genbank Nucleotide ID

HM149258;  

Genbank Protein ID

ADJ96868.1; 

Gene Name

 

Gene Synonyms/Alias

 

Protein Name

 

Protein Synonyms/Alias

SubName: Sox9a 

catfish) 

13013 

Temporarily unavailable 

The information of related literatures

1. K. Raghuveer and B. Senthilkumaran (2010) Isolation of sox9 duplicates in catfish. Reproduction 140(3): 477-87. 

Abstract
In vertebrates, sox9 is a transcription factor that plays a crucial role in testicular development and chondrogenesis. Here, we report cloning of isoforms of sox9 (sox9a and sox9b) from air-breathing catfish Clarias gariepinus, which undergoes an annual reproductive cycle. Tissue distribution pattern showed differential expression of sox9 duplicates, wherein both forms were highly expressed in brain and gonads. Furthermore, we observed a dimorphic expression pattern of sox9a and sox9b in both adult and developing gonads using RT-PCR, indicating that sox9a retained its function in testis while sox9b might have a new role to play in ovary. Changes in sox9 mRNA levels using real-time quantitative PCR (qRT-PCR) during the seasonal reproductive cycle revealed that sox9a transcript in testis was abundant during testicular recrudescence (during spermatogenesis), and its expression significantly decreased during spawning and post-spawning phases. Furthermore, treatments of human chorionic gonadotropin and 11-ketotestosterone in vitro up-regulated sox9a mRNA levels in the testicular slices at 12 and 24 h time points, suggesting that gonadotropins might stimulate sox9 expression. These results suggest that sox9 might have a plausible role in the entrainment of the testicular cycle. In contrast, during the ovarian cycle, sox9b mRNA levels gradually declined from preparatory to post-spawning phases. Immunohistochemical (IHC) data showed that, in testis, sox9 is detectable in Sertoli and spermatogonial cell types except spermatid/spermatozoa. In the ovary, it is localized in the ooplasm of primary and pre-vitellogenic oocytes. These results were further confirmed by whole-mount IHC and qRT-PCR. PMID: [20584991] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID

GO term

Evidence

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Interpro

IPR009071;    HMG_superfamily.
IPR022151;    Sox_N.
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Pfam

PF00505;    HMG_box;    1.
PF12444;    Sox_N;    1.
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SMART

SM00398;    HMG;    1.
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PROSITE

PS50118;    HMG_BOX_2;    1.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 460 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

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