SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000118 
UniProt Accession
Theoretical PI
6.21  
Molecular Weight
50275 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
bam 
Gene Synonyms/Alias
ORFNames=CG10422 
Protein Name
Protein bag-of-marbles 
Protein Synonyms/Alias
 
Organism
Drosophila melanogaster (Fruit fly) 
NCBI Taxonomy ID
7227 
Chromosome Location
chr:3R;21068761-21070884;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. C. Schulz, A. A. Kiger, S. I. Tazuke, Y. M. Yamashita, L. C. Pantalena-Filho, D. L. Jones, C. G. Wood and M. T. Fuller (2004) A misexpression screen reveals effects of bag-of-marbles and TGF beta class signaling on the Drosophila male germ-line stem cell lineage. Genetics 167(2): 707-23. 

Abstract
Male gametes are produced throughout reproductive life by a classic stem cell mechanism. However, little is known about the molecular mechanisms for lineage production that maintain male germ-line stem cell (GSC) populations, regulate mitotic amplification divisions, and ensure germ cell differentiation. Here we utilize the Drosophila system to identify genes that cause defects in the male GSC lineage when forcibly expressed. We conducted a gain-of-function screen using a collection of 2050 EP lines and found 55 EP lines that caused defects at early stages of spermatogenesis upon forced expression either in germ cells or in surrounding somatic support cells. Most strikingly, our analysis of forced expression indicated that repression of bag-of-marbles (bam) expression in male GSC is important for male GSC survival, while activity of the TGF beta signal transduction pathway may play a permissive role in maintenance of GSCs in Drosophila testes. In addition, forced activation of the TGF beta signal transduction pathway in germ cells inhibits the transition from the spermatogonial mitotic amplification program to spermatocyte differentiation. PMID: [15238523] 

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Figures for illustrating the function of this protein/gene
Function
Required to initiate both male and female gametogenesis.May regulate cystoblast cell divisions. 
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Subcellular Location
 
Tissue Specificity
In cystoblasts and/or very early cystocytes inovary and testis. 
Gene Ontology
GO IDGO termEvidence
GO:0045169 C:fusome IDA:FlyBase.
GO:0045170 C:spectrosome IDA:FlyBase.
GO:0000900 F:translation repressor activity, nucleic acid binding IDA:FlyBase.
GO:0001709 P:cell fate determination TAS:FlyBase.
GO:0001708 P:cell fate specification TAS:FlyBase.
GO:0007282 P:cystoblast division IMP:FlyBase.
GO:0048132 P:female germ-line stem cell division IMP:FlyBase.
GO:0030718 P:germ-line stem cell maintenance IDA:FlyBase.
GO:0030727 P:germarium-derived female germ-line cyst formation IMP:FlyBase.
GO:0007140 P:male meiosis TAS:FlyBase.
GO:0007275 P:multicellular organismal development IEA:UniProtKB-KW.
GO:0030721 P:spectrosome organization TAS:FlyBase.
GO:0007284 P:spermatogonial cell division IMP:FlyBase.
GO:0045479 P:vesicle targeting to fusome TAS:FlyBase.
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Interpro
Pfam
SMART
PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    442       Protein bag-of-marbles.
                             /FTId=PRO_0000064821.
COMPBIAS    402    434       Glu/Pro/Ser/Thr-rich (PEST region).
VARIANT      45     45       C -> S (in strain: Wpg6, Wpg8 and Wpg12).
VARIANT      54     54       G -> D (in strain: Zim10).
VARIANT     239    239       A -> S (in strain: fs(3)Neo61, Wpg1,
                             Wpg10, Wpg16, Wpg2, Wpg3, Zim10, Zim18,
                             Zim32 and Zim35).
VARIANT     243    243       F -> Y (in strain: Wpg4).
VARIANT     394    394       P -> R (in strain: Zim10).
CONFLICT    439    439       R -> A (in Ref. 1; CAA39662).
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Nucleotide Sequence
Length: 2505 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 442 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
bgcnBioGRID 
CycABioGRID 
mei-P26BioGRID 
bgcnBioGRID 
CycABioGRID 
mei-P26BioGRID 
TER94IntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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