SG00000120

SpermatogenesisOnline 1.0

Tag

Content

SG ID

SG00000120

UniProt Accession

EYA_DROME;Q05201;Q961V4;Q9VMC1;

Theoretical PI

6.42

Molecular Weight

80657 Da

Genbank Nucleotide ID

L08501; L08502; AE014134; AE014134; AY047539;

Genbank Protein ID

AAA28723.1; AAA28310.1; AAF52400.1; AAN10587.1; AAK77271.1;

Gene Name

eya

Gene Synonyms/Alias

cli

Protein Name

Developmental protein eyes absent

Protein Synonyms/Alias

EC=3.1.3.48 Protein Clift;

Organism

Drosophila melanogaster (Fruit fly)

NCBI Taxonomy ID

7227

Chromosome Location

chr:2L;6527447-6546972;-1

View in Ensembl genome browser

Function in Stage

Function in Cell Type

somatic_cyst_cell

Description

Temporarily unavailable

The information of related literatures

1. J. J. Fabrizio, M. Boyle and S. DiNardo (2003) A somatic role for eyes absent (eya) and sine oculis (so) in Drosophila spermatocyte development. Dev Biol 258(1): 117-28.

Abstract
Interactions between the soma and the germline are a conserved feature of spermatogenesis throughout the animal kingdom. In this report, we find that the transcription factors eyes absent (eya) and sine oculis (so), previously shown to play major roles during eye development [Cell 91 (1997), 881] are each required in the somatic cyst cells of the testis for proper Drosophila spermatocyte development. eya mutant testes exhibit degenerating young spermatocytes. Mosaic analysis reveals a somatic requirement for both eya and so, in that neither gene is required in the germline for spermatocyte development. Immunolocalization analysis supports this somatic role, since both proteins are localized within cyst cell nuclei as spermatocytes differentiate from amplifying spermatogonia. Using antibodies against known cyst cell markers, we demonstrate that cysts of degenerating spermatocytes in eya mutant testes are encysted, ruling out a role for eya in cyst cell viability. Finally, we have uncovered a genetic interaction between eya and so in the testis, suggesting that, as in the eye, eya and so may form a transcription complex responsible for the activation of target genes involved in cyst cell differentiation and spermatocyte development. PMID: [12781687]

Figures for illustrating the function of this protein/gene

Ref: J. J. Fabrizio, M. Boyle and S. DiNardo (2003) A somatic role for eyes absent (eya) and sine oculis (so) in Drosophila spermatocyte development. Dev Biol 258(1): 117-28. PMID: [12781687]

Function

Thought to play a role in transcription regulationduring organogenesis through its intrinsic protein phosphataseactivity. The phosphatase activity was shown in vitro. Appears tofunction together with So and Dac in eye development. Required forthe survival of eye progenitor cells at a critical stage inmorphogenesis.

Subcellular Location

Nucleus.

Tissue Specificity

Gene Ontology

GO ID	GO term	Evidence
GO:0005634	C:nucleus	IDA:UniProtKB.
GO:0046872	F:metal ion binding	IEA:UniProtKB-KW.
GO:0004725	F:protein tyrosine phosphatase activity	IDA:FlyBase.
GO:0001746	P:Bolwig's organ morphogenesis	IMP:FlyBase.
GO:0042051	P:compound eye photoreceptor development	IMP:FlyBase.
GO:0007455	P:eye-antennal disc morphogenesis	IMP:UniProtKB.
GO:0008584	P:male gonad development	IMP:FlyBase.
GO:0007498	P:mesoderm development	IMP:FlyBase.
GO:0009996	P:negative regulation of cell fate specification	IMP:FlyBase.
GO:0001744	P:optic lobe placode formation	IMP:FlyBase.
GO:0030707	P:ovarian follicle cell development	IMP:FlyBase.
GO:0007280	P:pole cell migration	TAS:FlyBase.
GO:0045944	P:positive regulation of transcription from RNA polymerase II promoter	IPI:FlyBase.
GO:0009416	P:response to light stimulus	IMP:FlyBase.
GO:0007435	P:salivary gland morphogenesis	IMP:FlyBase.
GO:0007283	P:spermatogenesis	IMP:FlyBase.
GO:0006351	P:transcription, DNA-dependent	TAS:FlyBase.

Interpro

IPR006545; EYA.
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Pfam

SMART

PROSITE

PRINTS

Created Date

18-Oct-2012

Record Type

Experiment identified

Protein sequence Annotation

CHAIN         1    766       Developmental protein eyes absent.
                             /FTId=PRO_0000218655.
COMPBIAS     46     57       Poly-Gln.
COMPBIAS     60     68       Poly-Gln.
COMPBIAS     92    108       Poly-Gly.
COMPBIAS    253    260       Poly-Ala.
COMPBIAS    268    271       Poly-Tyr.
COMPBIAS    305    311       Poly-Ala.
COMPBIAS    428    434       Poly-Ala.
ACT_SITE    499    499       Nucleophile (By similarity).
ACT_SITE    501    501       Proton donor (By similarity).
METAL       499    499       Magnesium (By similarity).
METAL       501    501       Magnesium (By similarity).
METAL       730    730       Magnesium (By similarity).
VAR_SEQ       1     23       MVTLMPYNYAAPRCGLIDKMIEP -> MLYNVPCYQNFSTL
                             DYY (in isoform 2).
                             /FTId=VSP_001500.
MUTAGEN     499    499       D->N: Highly reduced ectopic eye
                             induction and diminishes degree of
                             ommatidial restoration in eyeless
                             phenotype rescue assay.
MUTAGEN     676    676       S->A: Highly reduced ectopic eye
                             induction and diminishes degree of
                             ommatidial restoration in eyeless
                             phenotype rescue assay.
MUTAGEN     705    705       K->Q: Highly reduced ectopic eye
                             induction and diminishes degree of
                             ommatidial restoration in eyeless
                             phenotype rescue assay.
MUTAGEN     730    730       D->N: Highly reduced ectopic eye
                             induction.
MUTAGEN     734    734       E->Q: Highly reduced ectopic eye
                             induction.
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Nucleotide Sequence

Length: 3231 bp Go to nucleotide: FASTA

Protein Sequence

Length: 766 bp Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
3BP5H_DROME	pcs	BioGRID
A8Y4W5_DROME	rl	BioGRID
DECA_DROME	dpp	BioGRID
EGFR_DROME	Egfr	BioGRID
ERKA_DROME	rl	BioGRID
A8Y4W5_DROME	rl	BioGRID
DECA_DROME	dpp	BioGRID
EGFR_DROME	Egfr	BioGRID
ERKA_DROME	rl	BioGRID
EYA_DROME	eya	IntAct
EYA_DROME	eya	IntAct
KRAF1_DROME	phl	BioGRID
MAD_DROME	Mad	BioGRID
PAX6_DROME	ey	BioGRID
POK_DROME	aop	BioGRID
Q24027_DROME	dac	IntAct
Q53XF9_DROME	ey	BioGRID
Q59DQ1_DROME	ey	BioGRID
Q59DQ2_DROME	ey	BioGRID
Q59DY9_DROME	dac	BioGRID
Q59DZ0_DROME	dac	BioGRID
Q8IP19_DROME	dac	BioGRID
Q8IP20_DROME	dac	BioGRID
Q8IP21_DROME	dac	BioGRID
Q8MLW0_DROME	Egfr	BioGRID
Q9VJK6_DROME	dac	BioGRID
RAS1_DROME	Ras85D	BioGRID
SO_DROME	so	BioGRID
TSH_DROME	tsh	BioGRID
KRAF1_DROME	phl	BioGRID
MAD_DROME	Mad	BioGRID
PAX6_DROME	ey	BioGRID
POK_DROME	aop	BioGRID
Q2PDZ2_DROME	scaf6	BioGRID
Q53XF9_DROME	ey	BioGRID
Q59DQ1_DROME	ey	BioGRID
Q59DQ2_DROME	ey	BioGRID
Q59DY9_DROME	dac	BioGRID
Q59DZ0_DROME	dac	BioGRID
Q59E33_DROME	scaf6	BioGRID
Q86MQ9_DROME	scaf6	BioGRID
Q8IP19_DROME	dac	BioGRID
Q8IP20_DROME	dac	BioGRID
Q8IP21_DROME	dac	BioGRID
Q8MLW0_DROME	Egfr	BioGRID
Q8SXY2_DROME	CG1645	BioGRID
Q9VBD6_DROME	TwdlJ	BioGRID
Q9VJK6_DROME	dac	BioGRID
Q9VYG2_DROME	Bap60	BioGRID
RAS1_DROME	Ras85D	BioGRID
SMO_DROME	smo	BioGRID
SO_DROME	so	BioGRID

Other Protein-Protein interaction resources

String database

View Microarray data

Temporarily unavailable

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