SG00000121

SpermatogenesisOnline 1.0

Tag

Content

SG ID

SG00000121

UniProt Accession

CUP_DROME;Q9VMA3;O02432;

Theoretical PI

8.96

Molecular Weight

125672 Da

Genbank Nucleotide ID

AF004917; AE014134; AY069707;

Genbank Protein ID

AAB64427.1; AAF52418.3; AAL39852.1;

Gene Name

cup

Gene Synonyms/Alias

fs(2)cup

Protein Name

Protein cup

Protein Synonyms/Alias

Oskar ribonucleoprotein complex 147 kDa subunit;

Organism

Drosophila melanogaster (Fruit fly)

NCBI Taxonomy ID

7227

Chromosome Location

chr:2L;6663965-6674790;1

View in Ensembl genome browser

Function in Stage

Function in Cell Type

Description

Temporarily unavailable

The information of related literatures

1. C. Barreau, E. Benson and H. White-Cooper (2008) Comet and cup genes in Drosophila spermatogenesis. Biochem Soc Trans 36(Pt 3): 540-2.

Abstract
Post-meiotic transcription is widespread in mammalian spermatogenesis, but is generally believed to be absent from Drosophila spermatogenesis. Genes required during meiosis, in early spermatids or later in spermiogenesis are typically transcribed in primary spermatocytes in Drosophila. Their mRNAs are then stored in the cytoplasm until the protein product is needed. Recently, using in situ hybridization, we identified 17 Drosophila genes, collectively named 'comets' and 'cups', whose mRNAs are most abundant in, and localize to the distal ends of, elongating spermatids. Using a single-cyst quantitative RT-PCR (reverse transcription-PCR) assay, we confirmed this unusual expression pattern and conclusively demonstrate the existence of post-meiotic transcription in Drosophila spermatids. We found that transcription of comets and cups occurs just before protamines can be detected in spermatid nuclei. PMID: [18482002]

Figures for illustrating the function of this protein/gene

Ref: C. Barreau, E. Benson and H. White-Cooper (2008) Comet and cup genes in Drosophila spermatogenesis. Biochem Soc Trans 36(Pt 3): 540-2. PMID: [18482002]

Function

Adapter protein that plays a central role inlocalization of transcripts in the oocyte and in young embryosRequired to promote translation repression of oskar (osk) andnanos (nos) mRNAs. Acts by recruiting eIF-4E to the 3'-UTR of somemRNA targets and thereby preventing the interaction between eIF4-Eand eIF-4G, leading to prevent transcription.

Subcellular Location

Cytoplasm.

Tissue Specificity

Predominantly expressed in ovaries and in 0-2hours old embryos. Weakly expressed in testis. Expressed in youngembryos through stage 9, then it decreases throughout the rest ofembryogenesis. In ovaries, it is expressed in germ cellsthroughout pre-vitellogenic development, but is not expressed inthe somatic follicle cells. In germarial cysts, the protein (andnot the transcripts) is transported selectively into the oocyte.

Gene Ontology

GO ID	GO term	Evidence
GO:0000932	C:cytoplasmic mRNA processing body	IDA:FlyBase.
GO:0005875	C:microtubule associated complex	IDA:FlyBase.
GO:0003723	F:RNA binding	IDA:FlyBase.
GO:0045182	F:translation regulator activity	IDA:UniProtKB.
GO:0051297	P:centrosome organization	IMP:FlyBase.
GO:0051276	P:chromosome organization	TAS:FlyBase.
GO:0009953	P:dorsal/ventral pattern formation	IMP:FlyBase.
GO:0007143	P:female meiosis	IGI:UniProtKB.
GO:0008298	P:intracellular mRNA localization	IMP:FlyBase.
GO:0045132	P:meiotic chromosome segregation	IDA:UniProtKB.
GO:0007319	P:negative regulation of oskar mRNA translation	IDA:FlyBase.
GO:0007297	P:ovarian follicle cell migration	IMP:FlyBase.
GO:0007317	P:regulation of pole plasm oskar mRNA localization	IMP:FlyBase.
GO:0006355	P:regulation of transcription, DNA-dependent	IEA:UniProtKB-KW.
GO:0006351	P:transcription, DNA-dependent	IEA:UniProtKB-KW.

Interpro

IPR018862; eIF4E_transporter.
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Pfam

PF10477; EIF4E-T; 1.
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SMART

PROSITE

PRINTS

Created Date

18-Oct-2012

Record Type

Experiment identified

Protein sequence Annotation

CHAIN         1   1117       Protein cup.
                             /FTId=PRO_0000079561.
MOTIF       327    333       eIF-4E-binding 1.
MOTIF       363    369       eIF-4E-binding 2.
MOD_RES     263    263       Phosphoserine.
MOD_RES     270    270       Phosphoserine.
MOD_RES     347    347       Phosphoserine.
MOD_RES     350    350       Phosphoserine.
MOD_RES     503    503       Phosphothreonine.
MOD_RES     509    509       Phosphoserine.
MOD_RES     513    513       Phosphoserine.
MOD_RES     520    520       Phosphoserine.
MOD_RES     523    523       Phosphoserine.
MOD_RES     524    524       Phosphoserine.
MUTAGEN     327    327       Y->A: Strong reduction in interaction
                             with eIF4-E. Strong reduction
                             interaction; when associated with A-332
                             and A-348. Complete loss of interaction;
                             when associated with A-364 and A-368.
MUTAGEN     332    333       LM->AA: Reduction in interaction with
                             eIF4-E. Strong reduction interaction;
                             when associated with A-327.
MUTAGEN     364    364       L->A: Mild reduction in interaction with
                             eIF4-E. Complete loss of interaction;
                             when associated with A-327 and A-368.
MUTAGEN     368    368       L->A: Mild reduction in interaction with
                             eIF4-E. Complete loss of interaction;
                             when associated with A-327 and A-364.
CONFLICT    836    836       H -> Q (in Ref. 1; AAB64427).
CONFLICT   1030   1030       N -> K (in Ref. 1; AAB64427).
CONFLICT   1035   1035       K -> Q (in Ref. 1; AAB64427).
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Nucleotide Sequence

Length: 4041 bp Go to nucleotide: FASTA

Protein Sequence

Length: 1117 bp Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
CHIT3_DROME	Cht3	IntAct
A1Z6D7_DROME	gus	BioGRID
A1Z6D9_DROME	gus	BioGRID
A1Z6E0_DROME	gus	BioGRID
A1Z6E2_DROME	gus	BioGRID
A8DY78_DROME	Pdk	BioGRID
B7Z0Y2_DROME	mmd	BioGRID
C6TP17_DROME	CG15771-RB	BioGRID
C7LA75_DROME	Hsc70-4-RA	BioGRID
CHIT3_DROME	Cht3	BioGRID
DDX6_DROME	me31B	BioGRID
EXO1_DROME	tos	IntAct
HSP7D_DROME	Hsc70-4	BioGRID
IF4E_DROME	eIF-4E	BioGRID
NANOS_DROME	nos	BioGRID
O16797-1	_	MINT
O18409_DROME	aret	IntAct
O46070_DROME	EG:25E8.4	BioGRID
OTU_DROME	otu	BioGRID
PDK_DROME	Pdk	BioGRID
Q7JUY1_DROME	gus	BioGRID
Q7K187_DROME	Tango10	BioGRID
Q7KUY7_DROME	mmd	BioGRID
Q8IR14_DROME	Traf-like	BioGRID
Q8IRR4_DROME	CG15771	BioGRID
Q8SYG3_DROME	CG40045	BioGRID
Q8T3S1_DROME	srpk79D	BioGRID
Q960H6_DROME	Traf-like	BioGRID
Q9VAC4_DROME	CG7911	BioGRID
Q9VBJ6_DROME	CG14540	BioGRID
Q9VCD5_DROME	CG10694	IntAct
Q9VFT4_DROME	rin	BioGRID
Q9VID9_DROME	CG8677	BioGRID
Q9VNV0_DROME	srpk79D	BioGRID
Q9VPF9_DROME	CG5274	BioGRID
Q9VXG5_DROME	Traf-like	BioGRID
Q9VXL1_DROME	mmd	BioGRID
Q9W481_DROME	CG15771	BioGRID
Q9W5B3_DROME	eIF4E-7	BioGRID
RL22_DROME	RpL22	BioGRID
RL3_DROME	RpL3	BioGRID
RS26_DROME	RpS26	MINT
SMG_DROME	smg	IntAct
DDX6_DROME	me31B	IntAct
EXO1_DROME	tos	BioGRID
HSP7D_DROME	Hsc70-4	IntAct
IF4E_DROME	eIF-4E	BioGRID
NANOS_DROME	nos	BioGRID
O16797-1	_	IntAct
O46070_DROME	EG:25E8.4	IntAct
OTU_DROME	otu	BioGRID
PDK_DROME	Pdk	IntAct
Q8MLL7	_	MINT
Q8SXH3_DROME	eIF4AIII	IntAct
Q8SYG3_DROME	CG40045	IntAct
Q960H6_DROME	Traf-like	MINT
Q9VAC4_DROME	CG7911	IntAct
Q9VBJ6_DROME	CG14540	MINT
Q9VCD5_DROME	CG10694	BioGRID
Q9VFT4_DROME	rin	IntAct
Q9VID9_DROME	CG8677	IntAct
Q9VNV0_DROME	srpk79D	MINT
Q9VPF9_DROME	CG5274	IntAct
Q9VXL1_DROME	mmd	MINT
Q9VYV2	_	MINT
Q9W481_DROME	CG15771	MINT
Q9W5B3_DROME	eIF4E-7	IntAct
RL22_DROME	RpL22	IntAct
RS26_DROME	RpS26	BioGRID
SMG_DROME	smg	BioGRID

Other Protein-Protein interaction resources

String database

View Microarray data

Temporarily unavailable

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