SG00000123

SpermatogenesisOnline 1.0

Tag

Content

SG ID

SG00000123

UniProt Accession

PYRD_DROME;P32748;Q8INQ4;Q8INQ5;Q9VHP2;

Theoretical PI

UNDEFINED

Molecular Weight

44342 Da

Genbank Nucleotide ID

L00964; AE014297; AE014297; BT009968; BT010096; X17297;

Genbank Protein ID

AAB59185.1; AAF54260.1; AAN13391.2; AAQ22437.1; AAQ22565.1; CAA35184.1;

Gene Name

Dhod

Gene Synonyms/Alias

ORFNames=CG9741

Protein Name

Dihydroorotate dehydrogenase (quinone), mitochondrial

Protein Synonyms/Alias

DHOdehaseDihydroorotate oxidaseEC=1.3.5.2Flags: Precursor

Organism

Drosophila melanogaster (Fruit fly)

NCBI Taxonomy ID

7227

Chromosome Location

chr:3R;4477113-4478992;-1

View in Ensembl genome browser

Function in Stage

Function in Cell Type

Description

Temporarily unavailable

The information of related literatures

1. J. Yang, L. Porter and J. Rawls (1995) Expression of the dihydroorotate dehydrogenase gene, dhod, during spermatogenesis in Drosophila melanogaster. Mol Gen Genet 246(3): 334-41.

Abstract
The dhod gene encodes dihydroorotate dehydrogenase (DHOdehase), which catalyzes the fourth step of de novo pyrimidine biosynthesis. In addition to the common 1.5 kb dhod RNA expressed by embryos and females, adult males produce a group of slightly longer RNAs. Evidence is presented that the latter RNAs arise through transcription initiation at sites upstream from that of the common RNA and expression of these male-specific RNAs is limited to spermatogenesis. In situ hybridization analysis shows that these RNAs accumulate during spermatocyte growth and persist through meiosis and early spermatid differentiation. In contrast, DHOdehase activity is virtually absent in spermatocytes, meiotic cells, and in early spermatid cysts, then it becomes highly abundant in elongated spermatid cysts and disappears in late spermatogenesis. Thus, testis-limited expression of dhod conforms to a model proposed for other genes that function during spermiogenesis PMID: [7854318]

Figures for illustrating the function of this protein/gene

	Ref: J. Yang, L. Porter and J. Rawls (1995) Expression of the dihydroorotate dehydrogenase gene, dhod, during spermatogenesis in Drosophila melanogaster. Mol Gen Genet 246(3): 334-41. PMID: [7854318]
	Ref: J. Yang, L. Porter and J. Rawls (1995) Expression of the dihydroorotate dehydrogenase gene, dhod, during spermatogenesis in Drosophila melanogaster. Mol Gen Genet 246(3): 334-41. PMID: [7854318]
	Ref: J. Yang, L. Porter and J. Rawls (1995) Expression of the dihydroorotate dehydrogenase gene, dhod, during spermatogenesis in Drosophila melanogaster. Mol Gen Genet 246(3): 334-41. PMID: [7854318]

Function

Catalyzes the conversion of dihydroorotate to orotatewith quinone as electron acceptor (By similarity).

Subcellular Location

Mitochondrion inner membrane; Single-passmembrane protein (Potential).

Tissue Specificity

Gene Ontology

GO ID	GO term	Evidence
GO:0016021	C:integral to membrane	IEA:UniProtKB-KW.
GO:0005743	C:mitochondrial inner membrane	IEA:UniProtKB-SubCell.
GO:0005758	C:mitochondrial intermembrane space	IDA:FlyBase.
GO:0004152	F:dihydroorotate dehydrogenase activity	IDA:FlyBase.
GO:0004158	F:dihydroorotate oxidase activity	IEA:InterPro.
GO:0006207	P:'de novo' pyrimidine nucleobase biosynthetic process	IEA:InterPro.
GO:0044205	P:'de novo' UMP biosynthetic process	IEA:UniProtKB-UniPathway.

Interpro

IPR013785;    Aldolase_TIM.
IPR012135;    Dihydroorotate_DH_1_2.
IPR005719;    Dihydroorotate_DH_2.
IPR001295;    Dihydroorotate_DH_CS.
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Pfam

PF01180; DHO_dh; 1.
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SMART

PROSITE

PS00911; DHODEHASE_1; 1.
PS00912; DHODEHASE_2; 1.
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PRINTS

Created Date

18-Oct-2012

Record Type

Experiment identified

Protein sequence Annotation

TRANSIT       1      ?       Mitochondrion (Potential).
CHAIN         ?    405       Dihydroorotate dehydrogenase (quinone),
                             mitochondrial.
                             /FTId=PRO_0000029887.
TOPO_DOM      ?     20       Mitochondrial matrix (Potential).
TRANSMEM     21     39       Helical; (Potential).
TOPO_DOM     40    401       Mitochondrial intermembrane (Potential).
NP_BIND     103    107       FMN (By similarity).
NP_BIND     361    362       FMN (By similarity).
REGION      152    156       Substrate binding (By similarity).
REGION      215    220       Substrate binding (By similarity).
REGION      289    290       Substrate binding (By similarity).
ACT_SITE    218    218       Nucleophile (By similarity).
BINDING     107    107       Substrate (By similarity).
BINDING     127    127       FMN (By similarity).
BINDING     184    184       FMN (By similarity).
BINDING     215    215       FMN (By similarity).
BINDING     215    215       Substrate (By similarity).
BINDING     258    258       FMN (By similarity).
BINDING     288    288       FMN; via carbonyl oxygen (By similarity).
BINDING     311    311       FMN; via amide nitrogen (By similarity).
BINDING     340    340       FMN; via amide nitrogen (By similarity).
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Nucleotide Sequence

Length: 3078 bp Go to nucleotide: FASTA

Protein Sequence

Length: 405 bp Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
E1JHD6_DROME	bsk	BioGRID
E2QCS5_DROME	bsk	BioGRID
JNK_DROME	bsk	BioGRID
JNK_DROME	bsk	MINT
Q9VLI1_DROME	CG9465	IntAct
Q9VLI1_DROME	CG9465	BioGRID

Other Protein-Protein interaction resources

String database

View Microarray data

Temporarily unavailable

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