Tag Content
SG ID
SG00000130 
UniProt Accession
Theoretical PI
5.66  
Molecular Weight
318881 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
hyd 
Gene Synonyms/Alias
l(3)C43 
Protein Name
E3 ubiquitin-protein ligase hyd 
Protein Synonyms/Alias
EC=6.3.2.- Protein hyperplastic discs; 
Organism
Drosophila melanogaster (Fruit fly) 
NCBI Taxonomy ID
7227 
Chromosome Location
chr:3R;5540345-5551333;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. J. A. Pertceva, N. V. Dorogova, E. U. Bolobolova, O. O. Nerusheva, S. A. Fedorova and L. V. Omelyanchuk (2010) The role of Drosophila hyperplastic discs gene in spermatogenesis. Cell Biol Int 34(10): 991-6. 

Abstract
In Drosophila, the ubiquitin ligase Hyd (hyperplastic disc) is required for regulation of cell proliferation during development [Martin et al. (1977) Dev Biol 55, 213-232; Mansfield et al. (1994) Dev Biol 165, 507-526]. Earlier, we demonstrated that the Drosophila tumour suppressor Merlin participates not only in imaginal discs proliferation control, but also performs a separate Nebenkern structural function in Drosophila spermatogenesis [Dorogova et al. (2008) BMC Cell Biol 9, 1. Here, we show that the hyd mutants also have spermatogenesis defects PMID: [20604743] 

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Figures for illustrating the function of this protein/gene
Function
E3 ubiquitin-protein ligase which accepts ubiquitin froman E2 ubiquitin-conjugating enzyme in the form of a thioester andthen directly transfers the ubiquitin to targeted substrates (Bysimilarity). Required for regulation of cell proliferation inimaginal disks and germ cells. Acts as a negative regulator of hh,ci and dpp expression in the anterior of the eye disk. 
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Subcellular Location
Nucleus (Potential). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm IDA:FlyBase.
GO:0005634 C:nucleus IDA:FlyBase.
GO:0003723 F:RNA binding IEA:InterPro.
GO:0004842 F:ubiquitin-protein ligase activity IBA:RefGenome.
GO:0008270 F:zinc ion binding IEA:InterPro.
GO:0048749 P:compound eye development NAS:FlyBase.
GO:0007455 P:eye-antennal disc morphogenesis IMP:FlyBase.
GO:0008585 P:female gonad development IMP:FlyBase.
GO:0007446 P:imaginal disc growth IMP:FlyBase.
GO:0007112 P:male meiosis cytokinesis IMP:FlyBase.
GO:0045879 P:negative regulation of smoothened signaling pathway NAS:FlyBase.
GO:0042787 P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process IBA:RefGenome.
GO:0030162 P:regulation of proteolysis NAS:FlyBase.
GO:0007289 P:spermatid nucleus differentiation IMP:FlyBase.
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Interpro
IPR024725;    E3_UbLigase_EDD_UBA.
IPR000569;    HECT.
IPR002004;    PABP_HYD.
IPR009091;    Reg_csome_cond/b-lactamase_inh.
IPR015940;    UBA/transl_elong_EF1B_N_euk.
IPR003126;    Znf_N-recognin.
IPR013993;    Znf_N-recognin_met.
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Pfam
PF11547;    E3_UbLigase_EDD;    1.
PF00632;    HECT;    1.
PF00658;    PABP;    1.
PF02207;    zf-UBR;    1.
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SMART
SM00119;    HECTc;    1.
SM00517;    PolyA;    1.
SM00396;    ZnF_UBR1;    1.
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PROSITE
PS50237;    HECT;    1.
PS51309;    PABC;    1.
PS50030;    UBA;    1.
PS51157;    ZF_UBR;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   2885       E3 ubiquitin-protein ligase hyd.
                             /FTId=PRO_0000084107.
DOMAIN      154    196       UBA.
DOMAIN     2484   2561       PABC.
DOMAIN     2782   2885       HECT.
ZN_FING    1217   1285       UBR-type.
COMPBIAS    962   1112       Ser-rich.
ACT_SITE   2854   2854       Glycyl thioester intermediate (By
                             similarity).
MOD_RES     628    628       Phosphoserine.
MOD_RES     631    631       Phosphoserine.
MOD_RES     967    967       Phosphoserine.
MOD_RES    1362   1362       Phosphoserine.
MOD_RES    2037   2037       Phosphoserine.
MOD_RES    2183   2183       Phosphoserine.
MOD_RES    2574   2574       Phosphoserine.
CONFLICT    125    125       T -> A (in Ref. 1; AAB88625).
CONFLICT    277    277       T -> A (in Ref. 1; AAB88625).
CONFLICT    294    294       S -> P (in Ref. 1; AAB88625).
CONFLICT    377    377       S -> A (in Ref. 1; AAB88625).
CONFLICT    383    383       Y -> C (in Ref. 1; AAB88625).
CONFLICT    386    386       R -> G (in Ref. 1; AAB88625).
CONFLICT    397    397       T -> A (in Ref. 1; AAB88625).
CONFLICT    407    407       S -> C (in Ref. 1; AAB88625).
CONFLICT    657    657       A -> G (in Ref. 1; AAB88625).
CONFLICT    741    759       EDVQIFRTAMSTRGPDWLQ -> KMSDISHCYVTRLLA
                             (in Ref. 1; AAB88625).
CONFLICT   1490   1490       V -> A (in Ref. 1; AAB88625).
CONFLICT   1497   1497       E -> Q (in Ref. 1; AAB88625).
CONFLICT   1503   1505       EKR -> QKQ (in Ref. 1; AAB88625).
CONFLICT   1557   1559       MSS -> CHR (in Ref. 1; AAB88625).
CONFLICT   1625   1625       R -> G (in Ref. 1; AAB88625).
CONFLICT   1641   1641       R -> G (in Ref. 1; AAB88625).
CONFLICT   1784   1784       P -> R (in Ref. 1; AAB88625).
CONFLICT   1978   1978       T -> S (in Ref. 1; AAB88625).
CONFLICT   2037   2037       S -> T (in Ref. 1; AAB88625).
CONFLICT   2096   2096       G -> A (in Ref. 1; AAB88625).
CONFLICT   2167   2167       G -> GELRVYAVNNLQTLNFQSTR (in Ref. 1;
                             AAB88625).
CONFLICT   2239   2272       SASNLSTAEQAKCDTQYQKSTSDHLLLFPARGSQ -> RLQ
                             IYRPQNKPNVIHNIKSQHQTTCYYFLLVVLS (in Ref.
                             1).
CONFLICT   2280   2284       ELPSW -> GIAPC (in Ref. 1; AAB88625).
CONFLICT   2290   2291       RW -> A (in Ref. 1; AAB88625).
CONFLICT   2301   2301       V -> P (in Ref. 1; AAB88625).
CONFLICT   2439   2440       SR -> T (in Ref. 1; AAB88625).
CONFLICT   2601   2601       T -> I (in Ref. 1; AAB88625).
CONFLICT   2646   2646       G -> R (in Ref. 1; AAB88625).
CONFLICT   2711   2728       AVTSSNIFEYVRRYTEYR -> VSHRVIYSSTSDAIQNID
                             (in Ref. 1; AAB88625).
CONFLICT   2814   2814       D -> H (in Ref. 1; AAB88625).
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Nucleotide Sequence
Length: 9053 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 2885 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
DIP2String 
hhBioGRID 
DIP2String 
hhBioGRID 
Sema-1aIntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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