SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000132 
UniProt Accession
Theoretical PI
8.8  
Molecular Weight
44402 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
mei-S332 
Gene Synonyms/Alias
ORFNames=CG5303 
Protein Name
Shugoshin 
Protein Synonyms/Alias
Meiotic protein S332; 
Organism
Drosophila melanogaster (Fruit fly) 
NCBI Taxonomy ID
7227 
Chromosome Location
chr:2R;18009606-18012071;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. A. W. Kerrebrock, D. P. Moore, J. S. Wu and T. L. Orr-Weaver (1995) Mei-S332, a Drosophila protein required for sister-chromatid cohesion, can localize to meiotic centromere regions. Cell 83(2): 247-56. 

Abstract
Mutations in the Drosophila mei-S332 gene cause premature separation of the sister chromatids in late anaphase of meiosis I. Therefore, the mei-S332 protein was postulated to hold the centromere regions of sister chromatids together until anaphase II. The mei-S332 gene encodes a novel 44 kDa protein. Mutations in mei-S332 that differentially affect function in males or females map to distinct domains of the protein. A fusion of mei-S332 to the green fluorescent protein (GFP) is fully functional and localizes specifically to the centromere region of meiotic chromosomes. When sister chromatids separate at anaphase II, mei-S332-GFP disappears from the chromosomes, suggesting that the destruction or release of this protein is required for sister-chromatid separation. PMID: [7585942] 

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Figures for illustrating the function of this protein/gene
Function
Plays a central role in chromosome cohesion duringmeiosis and mitosis by preventing premature dissociation ofcohesin complex from centromeres after prophase, when most ofcohesin complex dissociates from chromosomes arms. May act byprotecting or Rad21 from cleavage by Sse/separase. Required duringmeiosis in both males and females. 
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Subcellular Location
Nucleus. Chromosome, centromere.Note=Localizes to the centromere of meiotic and mitoticchromosomes. During meiosis, it assembles onto centromeres duringprometaphase I, remains attached until anaphase II, during whichit is displaced from centromeres without being degraded.Localization onto centromeres is independent of kinetochoreformation and cohesin complex. 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0000780 C:condensed nuclear chromosome, centromeric region IDA:FlyBase.
GO:0051301 P:cell division IEA:UniProtKB-KW.
GO:0007143 P:female meiosis IMP:FlyBase.
GO:0007065 P:male meiosis sister chromatid cohesion IEP:FlyBase.
GO:0007132 P:meiotic metaphase I TAS:FlyBase.
GO:0007067 P:mitosis IEA:UniProtKB-KW.
GO:0007275 P:multicellular organismal development IEA:UniProtKB-KW.
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Interpro
IPR011515;    Shugoshin_C.
IPR011516;    Shugoshin_N.
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Pfam
PF07557;    Shugoshin_C;    1.
PF07558;    Shugoshin_N;    1.
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SMART
PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    401       Shugoshin.
                             /FTId=PRO_0000055443.
COILED        3     49       Potential.
MOD_RES     124    124       Phosphoserine; by ial (Probable).
MOD_RES     125    125       Phosphoserine; by ial (Probable).
MOD_RES     126    126       Phosphoserine; by ial (Probable).
MOD_RES     331    331       Phosphothreonine; by PLK1 (Probable).
MUTAGEN      13     13       N->I: In mei-S332-9; induces
                             missegregation of chromatids during
                             meiosis.
MUTAGEN      28     28       G->D: In mei-S332-3; induces
                             missegregation of chromatids during
                             meiosis.
MUTAGEN      34     34       V->E: In mei-S332-8; induces
                             missegregation of chromatids during
                             meiosis.
MUTAGEN     112    112       L->M: In mei-S332-4; induces
                             missegregation of chromatids during
                             meiosis; when associated with H-.
MUTAGEN     124    126       SSS->AAA: Does not stably associate with
                             centromeres during mitosis.
MUTAGEN     234    234       S->A: Does not affect phosphorylation.
MUTAGEN     277    277       S->F: In mei-S332-5; induces
                             missegregation of chromatids during
                             meiosis.
MUTAGEN     331    331       T->A: Affects phosphorylation and
                             localization to centromeres.
MUTAGEN     375    375       C->Y: In mei-S332-2; induces
                             missegregation of chromatids during
                             meiosis.
MUTAGEN     377    377       P->H: In mei-S332-4; induces
                             missegregation of chromatids during
                             meiosis; when associated with M-112.
MUTAGEN     382    382       E->K: In mei-S332-10; induces
                             missegregation of chromatids during
                             meiosis.
MUTAGEN     384    384       S->R: In mei-S332-6; induces
                             missegregation of chromatids during
                             meiosis.
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Nucleotide Sequence
Length: 1540 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 401 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
CG15073-RABioGRID 
CG4820BioGRID 
Anxb11MINT 
ple-RBBioGRID 
ord-RABioGRID 
CG1553BioGRID 
grauBioGRID 
Ppi20BioGRID 
PrmMINT 
ordBioGRID 
pleMINT 
yinBioGRID 
CG18011BioGRID 
CG5151BioGRID 
Xrp1BioGRID 
CkIIalpha-i3BioGRID 
CG17802BioGRID 
CG5089BioGRID 
CG1635BioGRID 
kat-60L1IntAct 
_MINT 
_MINT 
_MINT 
CG11882BioGRID 
rapsMINT 
CG5882MINT 
CG14546BioGRID 
CG3517BioGRID 
Xrp1BioGRID 
CG6654MINT 
CG4820BioGRID 
B4MINT 
CG16812BioGRID 
CG5973MINT 
tombBioGRID 
CG2926BioGRID 
CG3225BioGRID 
CG7386MINT 
CG5151BioGRID 
CG13733BioGRID 
CG9099MINT 
sofeBioGRID 
Rcd5BioGRID 
Mfap1BioGRID 
CkIIalpha-i3BioGRID 
_MINT 
CG17168BioGRID 
CG15073-RAIntAct 
PrmBioGRID 
PrmBioGRID 
Anxb11BioGRID 
grauIntAct 
Ppi20IntAct 
PrmBioGRID 
PrmBioGRID 
pleIntAct 
yinIntAct 
CG18011IntAct 
CG17802IntAct 
CG5089IntAct 
CG1635IntAct 
CG11882IntAct 
rapsBioGRID 
CG5882BioGRID 
CG14546IntAct 
CG3517IntAct 
Xrp1IntAct 
CG6654BioGRID 
CG4820IntAct 
B4BioGRID 
CG16812IntAct 
CG5973BioGRID 
tombIntAct 
CG2926IntAct 
CG3225IntAct 
CG7386BioGRID 
CG5151IntAct 
CG13733IntAct 
CG9099BioGRID 
Anxb11BioGRID 
sofeIntAct 
Rcd5IntAct 
Mfap1IntAct 
CkIIalpha-i3IntAct 
CG17168IntAct 
mei-S332IntAct 
mei-S332IntAct 
king-tubbyBioGRID 
pleDIP 
pleBioGRID 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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