SG ID

SG00000180 

UniProt Accession

P91945_DROME;P91945;  

Theoretical PI

9.26  

Molecular Weight

92440 Da  

Genbank Nucleotide ID

U89264;  

Genbank Protein ID

AAB49460.1; 

Gene Name

Klp67A 

Gene Synonyms/Alias

ORFNames=CG10923 

Protein Name

 

Protein Synonyms/Alias

SubName: Kinesin like protein 67a 

Drosophila melanogaster (Fruit fly) 

7227 

Temporarily unavailable 

The information of related literatures

1. M. S. Savoian, M. K. Gatt, M. G. Riparbelli, G. Callaini and D. M. Glover (2004) Drosophila Klp67A is required for proper chromosome congression and segregation during meiosis I. J Cell Sci 117(Pt 16): 3669-77. 

Abstract
Drosophila Klp67A belongs to the Kip3 subfamily of Kinesin-type microtubule catastrophe factors. In primary spermatocytes, loss of klp67A leads to defects in karyokinesis and cytokinesis. We show that these cells formed disorganised, bipolar spindles that contained increased numbers of microtubules. The kinetochore fibres were wavy and bent, whereas astral microtubules appeared abnormally robust and formed cortical bundles. Time-lapse studies revealed that during biorientation, the chromosomes in klp67A mutant cells continued to reorient for about twice as long as those in control cells. Metaphase plates were poorly defined in the mutants and often formed at non-equatorial positions. Consistent with the above abnormalities in chromosome congression, we found that in wild-type cells Klp67A associated with prometaphase/metaphase kinetochores before redistributing to the central spindle at anaphase onset. Although the timing of this redistribution of kinetochores argues against a role in anaphase chromosome segregation, dyads in the mutants disjoined but exhibited greatly diminished poleward velocities. They travelled on average at approximately 34% of the velocity of their wild-type counterparts and often decondensed at non-polar locations. Hypomorphic mutations of klp67A may lead to segregation defects. PMID: [15252134] 

2. R. Gandhi, S. Bonaccorsi, D. Wentworth, S. Doxsey, M. Gatti and A. Pereira (2004) The Drosophila kinesin-like protein KLP67A is essential for mitotic and male meiotic spindle assembly. Mol Biol Cell 15(1): 121-31. 

Abstract
We have performed a mutational analysis together with RNA interference to determine the role of the kinesin-like protein KLP67A in Drosophila cell division. During both mitosis and male meiosis, Klp67A mutations cause an increase in MT length and disrupt discrete aspects of spindle assembly, as well as cytokinesis. Mutant cells exhibit greatly enlarged metaphase spindle as a result of excessive MT polymerization. The analysis of both living and fixed cells also shows perturbations in centrosome separation, chromosome segregation, and central spindle assembly. These data demonstrate that the MT plus end-directed motor KLP67A is essential for spindle assembly during mitosis and male meiosis and suggest that the regulation of MT plus-end polymerization is a key determinant of spindle architecture throughout cell division. PMID: [13679514] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005737	C:cytoplasm	IDA:FlyBase.
GO:0005871	C:kinesin complex	ISS:FlyBase.
GO:0005874	C:microtubule	IEA:UniProtKB-KW.
GO:0005634	C:nucleus	IDA:FlyBase.
GO:0005524	F:ATP binding	IEA:UniProtKB-KW.
GO:0003777	F:microtubule motor activity	ISS:FlyBase.
GO:0051299	P:centrosome separation	IMP:FlyBase.
GO:0000910	P:cytokinesis	IMP:FlyBase.
GO:0016346	P:male meiotic chromosome movement towards spindle pole	IMP:FlyBase.
GO:0046785	P:microtubule polymerization	IMP:FlyBase.
GO:0007052	P:mitotic spindle organization	IMP:FlyBase.
GO:0031134	P:sister chromatid biorientation	IMP:FlyBase.
GO:0051225	P:spindle assembly	IMP:FlyBase.
GO:0051231	P:spindle elongation	IMP:FlyBase.

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Interpro

IPR001752;    Kinesin_motor_dom.
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Pfam

PF00225;    Kinesin;    1.
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SMART

SM00129;    KISc;    1.
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PROSITE

PS50067;    KINESIN_MOTOR_DOMAIN2;    1.
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PRINTS

PR00380;    KINESINHEAVY.;   
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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 2963 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 814 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

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