SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000187 
UniProt Accession
Theoretical PI
8.62  
Molecular Weight
21354 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Rac1 
Gene Synonyms/Alias
RacA 
Protein Name
Ras-related protein Rac1 
Protein Synonyms/Alias
Flags: Precursor 
Organism
Drosophila melanogaster (Fruit fly) 
NCBI Taxonomy ID
7227 
Chromosome Location
chr:3L;1300879-1302683;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. K. Raymond, E. Bergeret, A. Avet-Rochex, R. Griffin-Shea and M. O. Fauvarque (2004) A screen for modifiers of RacGAP(84C) gain-of-function in the Drosophila eye revealed the LIM kinase Cdi/TESK1 as a downstream effector of Rac1 during spermatogenesis. J Cell Sci 117(Pt 13): 2777-89. 

Abstract
In Drosophila, RotundRacGAP/RacGAP(84C) is critical to retinal organisation and spermatogenesis. We show that eye-directed expression of RacGAP(84C) or its GTPase activating protein (GAP) domain induces a dominant rough eye phenotype which we used as a starting point in a gain-of-function screen to identify new partners of RacGAP(84C). Proteins known to function in Ras, Rho and Rac signalling were identified confirming the essential role of RacGAP(84C) in crosstalk between GTPases. Other potential RacGAP(84C) partners identified by the screen are implicated in signal transduction, DNA remodelling, cytoskeletal organisation, membrane trafficking and spermatogenesis. This latter class includes the serine/threonine kinase Center divider (Cdi), which is homologous to the human LIM kinase, Testis specific kinase 1 (TESK1), involved in cytoskeleton control through Cofilin phosphorylation. Eye-directed expression of cdi strongly suppressed the phenotypes induced by either RacGAP(84C) gain-of-function or by the dominant negative form of Rac1, Rac1N17. These results are consistent with Cdi being a specific downstream target of Rac1. We showed that Rac1 and cdi are both expressed in Drosophila testis and that homozygous Rac1 mutants exhibit poor fertility that is further reduced by introducing a cdi loss-of-function mutation in trans. Thus, results from a misexpression screen in the eye led us to a putative novel Rac1-Cdi-Cofilin pathway, regulated by RacGAP(84C), coordinating Drosophila spermatogenesis. PMID: [15169836] 

Back to Top
Figures for illustrating the function of this protein/gene
Function
Involved in axon outgrowth and myoblast fusion. Plays arole in regulating dorsal closure during embryogenesis. 
Back to Top
Subcellular Location
Cell membrane; Lipid-anchor; Cytoplasmicside (Potential). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005829 C:cytosol TAS:Reactome.
GO:0005886 C:plasma membrane IEA:UniProtKB-SubCell.
GO:0016028 C:rhabdomere IDA:FlyBase.
GO:0005525 F:GTP binding IEA:UniProtKB-KW.
GO:0003924 F:GTPase activity ISS:FlyBase.
GO:0051017 P:actin filament bundle assembly IMP:FlyBase.
GO:0034334 P:adherens junction maintenance IMP:FlyBase.
GO:0048675 P:axon extension IMP:FlyBase.
GO:0007413 P:axonal fasciculation IMP:FlyBase.
GO:0007298 P:border follicle cell migration IMP:FlyBase.
GO:0035212 P:cell competition in a multicellular organism IMP:FlyBase.
GO:0048813 P:dendrite morphogenesis TAS:FlyBase.
GO:0046843 P:dorsal appendage formation IMP:FlyBase.
GO:0046664 P:dorsal closure, amnioserosa morphology change IMP:FlyBase.
GO:0007394 P:dorsal closure, elongation of leading edge cells IMP:FlyBase.
GO:0042067 P:establishment of ommatidial planar polarity NAS:FlyBase.
GO:0007390 P:germ-band shortening IMP:FlyBase.
GO:0008347 P:glial cell migration IMP:FlyBase.
GO:0008258 P:head involution IMP:FlyBase.
GO:0007516 P:hemocyte development IMP:FlyBase.
GO:0035099 P:hemocyte migration IMP:FlyBase.
GO:0035320 P:imaginal disc-derived wing hair site selection IMP:FlyBase.
GO:0002433 P:immune response-regulating cell surface receptor signaling pathway involved in phagocytosis IDA:FlyBase.
GO:0007254 P:JNK cascade IMP:FlyBase.
GO:0030032 P:lamellipodium assembly IMP:FlyBase.
GO:0035011 P:melanotic encapsulation of foreign target IMP:FlyBase.
GO:0008078 P:mesodermal cell migration IMP:FlyBase.
GO:0007017 P:microtubule-based process NAS:FlyBase.
GO:0016335 P:morphogenesis of larval imaginal disc epithelium TAS:FlyBase.
GO:0008045 P:motor axon guidance IMP:FlyBase.
GO:0048747 P:muscle fiber development IMP:FlyBase.
GO:0007520 P:myoblast fusion IMP:FlyBase.
GO:0051450 P:myoblast proliferation IMP:FlyBase.
GO:0010593 P:negative regulation of lamellipodium assembly IMP:FlyBase.
GO:0007422 P:peripheral nervous system development IMP:FlyBase.
GO:0006911 P:phagocytosis, engulfment IMP:FlyBase.
GO:0071902 P:positive regulation of protein serine/threonine kinase activity IDA:UniProtKB.
GO:0050770 P:regulation of axonogenesis IGI:FlyBase.
GO:0048814 P:regulation of dendrite morphogenesis IMP:FlyBase.
GO:0045610 P:regulation of hemocyte differentiation IMP:FlyBase.
GO:0051963 P:regulation of synapse assembly IMP:FlyBase.
GO:0006974 P:response to DNA damage stimulus IMP:FlyBase.
GO:0042052 P:rhabdomere development IMP:FlyBase.
GO:0007435 P:salivary gland morphogenesis IMP:FlyBase.
GO:0007264 P:small GTPase mediated signal transduction IEA:InterPro.
GO:0007426 P:tracheal outgrowth, open tracheal system IGI:FlyBase.
GO:0007419 P:ventral cord development IMP:FlyBase.
Back to Top
Interpro
IPR005225;    Small_GTP-bd_dom.
IPR001806;    Small_GTPase.
IPR003578;    Small_GTPase_Rho.
Back to Top
Pfam
PF00071;    Ras;    1.
Back to Top
SMART
SM00174;    RHO;    1.
Back to Top
PROSITE
PS51420;    RHO;    1.
Back to Top
PRINTS
PR00449;    RASTRNSFRMNG.;   
Back to Top
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    189       Ras-related protein Rac1.
                             /FTId=PRO_0000198893.
PROPEP      190    192       Removed in mature form (By similarity).
                             /FTId=PRO_0000281244.
NP_BIND      10     17       GTP (By similarity).
NP_BIND      57     61       GTP (By similarity).
NP_BIND     115    118       GTP (By similarity).
MOTIF        32     40       Effector region (Potential).
MOD_RES     189    189       Cysteine methyl ester (By similarity).
LIPID       189    189       S-geranylgeranyl cysteine (By
                             similarity).
CONFLICT    135    135       A -> V (in Ref. 1; AAA62870).
CONFLICT    143    146       LAMA -> SGHG (in Ref. 2; AAA67040).
Back to Top
Nucleotide Sequence
Length: 705 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 192 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
VangBioGRID 
PknIntAct 
tumBioGRID 
Rho1BioGRID 
LarBioGRID 
MbsBioGRID 
MbsBioGRID 
MbsBioGRID 
CdGAPrIntAct 
ChcIntAct 
Dcr-1IntAct 
DIP2String 
dshBioGRID 
bskBioGRID 
bskBioGRID 
argosBioGRID 
HemBioGRID 
hepBioGRID 
bskBioGRID 
JraBioGRID 
phlBioGRID 
LarBioGRID 
slprBioGRID 
ckBioGRID 
zipBioGRID 
par-6IntAct 
ninaEBioGRID 
plexBBioGRID 
pkBioGRID 
chicBioGRID 
PakIntAct 
zipBioGRID 
zipBioGRID 
MtlBioGRID 
necBioGRID 
nopoIntAct 
CG12183IntAct 
tkvBioGRID 
msnBioGRID 
msnBioGRID 
msnBioGRID 
trioBioGRID 
roboBioGRID 
RhoGAP93BIntAct 
trioBioGRID 
tkvBioGRID 
nmoBioGRID 
MbsBioGRID 
trioBioGRID 
trioBioGRID 
CG7511IntAct 
CG30115IntAct 
Pp2A-29BIntAct 
MbsBioGRID 
nmoBioGRID 
Vps35IntAct 
tkvBioGRID 
Hex-AIntAct 
WASpIntAct 
mbcBioGRID 
Pak3IntAct 
pucBioGRID 
tkvBioGRID 
RecQ4BioGRID 
rheaIntAct 
MbsBioGRID 
MbsBioGRID 
RhoGAP18BIntAct 
rokBioGRID 
GrafIntAct 
radIntAct 
msnBioGRID 
roboBioGRID 
CG2278IntAct 
Traf6IntAct 
Pp2A-29BBioGRID 
VangBioGRID 
PknBioGRID 
PknBioGRID 
PknBioGRID 
PknBioGRID 
PknBioGRID 
tumBioGRID 
CG30115BioGRID 
Rho1BioGRID 
PknBioGRID 
LarBioGRID 
MbsBioGRID 
MbsBioGRID 
MbsBioGRID 
PakBioGRID 
CdGAPrBioGRID 
ChcBioGRID 
Dcr-1BioGRID 
DIP2String 
dshBioGRID 
PknBioGRID 
bskBioGRID 
CdGAPrBioGRID 
CdGAPrBioGRID 
bskBioGRID 
btlBioGRID 
HemBioGRID 
hepBioGRID 
thIntAct 
bskBioGRID 
JraBioGRID 
phlBioGRID 
LarBioGRID 
slprBioGRID 
ckBioGRID 
zipBioGRID 
par-6BioGRID 
ninaEBioGRID 
plexBBioGRID 
pkBioGRID 
chicBioGRID 
RhoGAP18BBioGRID 
stumpsBioGRID 
PakIntAct 
zipBioGRID 
zipBioGRID 
MtlBioGRID 
necBioGRID 
nopoBioGRID 
CG12183BioGRID 
tkvBioGRID 
CG33275BioGRID 
CG33275BioGRID 
CG33275BioGRID 
msnBioGRID 
msnBioGRID 
msnBioGRID 
trioBioGRID 
roboBioGRID 
Vps35BioGRID 
trioBioGRID 
WASpBioGRID 
bnlBioGRID 
tkvBioGRID 
nmoBioGRID 
MbsBioGRID 
trioBioGRID 
trioBioGRID 
mbcIntAct 
MbsBioGRID 
nmoBioGRID 
tkvBioGRID 
Hex-ABioGRID 
RecQ4IntAct 
WASpBioGRID 
mbcBioGRID 
RhoGAP93BBioGRID 
bnlBioGRID 
Pak3BioGRID 
stumpsBioGRID 
stumpsBioGRID 
pucBioGRID 
PakBioGRID 
tkvBioGRID 
rheaBioGRID 
MbsBioGRID 
MbsBioGRID 
RhoGAP18BBioGRID 
RhoGAP18BBioGRID 
rokBioGRID 
GrafBioGRID 
radBioGRID 
msnBioGRID 
roboBioGRID 
Vps35BioGRID 
spriBioGRID 
Hex-ABioGRID 
CG2278BioGRID 
Traf6BioGRID 
Rac2BioGRID 
RhoGAP68FBioGRID 
RacGAP84CBioGRID 
Rho1BioGRID 
spriBioGRID 
sqhBioGRID 
RhoGAP68FIntAct 
RacGAP84CBioGRID 
Rho1BioGRID 
spirIntAct 
spriIntAct 
sqhBioGRID 
vavIntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
Comments