SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000193 
UniProt Accession
Theoretical PI
8.83  
Molecular Weight
143299 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
jar 
Gene Synonyms/Alias
Mhc95F 
Protein Name
Myosin heavy chain 95F 
Protein Synonyms/Alias
95F MHC; Protein jaguar; 
Organism
Drosophila melanogaster (Fruit fly) 
NCBI Taxonomy ID
7227 
Chromosome Location
chr:3R;20078548-20096953;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. J. L. Hicks, W. M. Deng, A. D. Rogat, K. G. Miller and M. Bownes (1999) Class VI unconventional myosin is required for spermatogenesis in Drosophila. Mol Biol Cell 10(12): 4341-53. 

Abstract
We have identified partial loss of function mutations in class VI unconventional myosin, 95F myosin, which results in male sterility. During spermatogenesis the germ line precursor cells undergo mitosis and meiosis to form a bundle of 64 spermatids. The spermatids remain interconnected by cytoplasmic bridges until individualization. The process of individualization involves the formation of a complex of cytoskeletal proteins and membrane, the individualization complex (IC), around the spermatid nuclei. This complex traverses the length of each spermatid resolving the shared membrane into a single membrane enclosing each spermatid. We have determined that 95F myosin is a component of the IC whose function is essential for individualization. In wild-type testes, 95F myosin localizes to the leading edge of the IC. Two independent mutations in 95F myosin reduce the amount of 95F myosin in only a subset of tissues, including the testes. This reduction of 95F myosin causes male sterility as a result of defects in spermatid individualization. Germ line transformation with the 95F myosin heavy chain cDNA rescues the male sterility phenotype. IC movement is aberrant in these 95F myosin mutants, indicating a critical role for 95F myosin in IC movement. This report is the first identification of a component of the IC other than actin. We propose that 95F myosin is a motor that participates in membrane reorganization during individualization. PMID: [10588662] 

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Figures for illustrating the function of this protein/gene
Ref: J. L. Hicks, W. M. Deng, A. D. Rogat, K. G. Miller and M. Bownes (1999) Class VI unconventional myosin is required for spermatogenesis in Drosophila. Mol Biol Cell 10(12): 4341-53. PMID: [10588662]
Ref: J. L. Hicks, W. M. Deng, A. D. Rogat, K. G. Miller and M. Bownes (1999) Class VI unconventional myosin is required for spermatogenesis in Drosophila. Mol Biol Cell 10(12): 4341-53. PMID: [10588662]
Ref: J. L. Hicks, W. M. Deng, A. D. Rogat, K. G. Miller and M. Bownes (1999) Class VI unconventional myosin is required for spermatogenesis in Drosophila. Mol Biol Cell 10(12): 4341-53. PMID: [10588662]
Ref: J. L. Hicks, W. M. Deng, A. D. Rogat, K. G. Miller and M. Bownes (1999) Class VI unconventional myosin is required for spermatogenesis in Drosophila. Mol Biol Cell 10(12): 4341-53. PMID: [10588662]
Function
Myosin is a protein that binds to actin and has ATPaseactivity that is activated by actin. Together CLIP-190 and jar maycoordinate the interaction between the actin and microtubulecytoskeleton. May link endocytic vesicles to microtubules and maybe involved in transport in the early embryo and in the dynamicprocess of dorsal closure. It is believed that its functionchanges during the life cycle. 
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Subcellular Location
Cytoplasm, cytoskeleton. Note=Microtubule-associated. 
Tissue Specificity
Isoform B is present at a higher level in thehead and gonads than in the thoraxes. Isoform 145 kDa is foundonly in the head. CLIP-190 and jar are coexpressed at severaltimes in development and in a number of tissues, includingembryonic axonal neuron processes and posterior pole. 
Gene Ontology
GO IDGO termEvidence
GO:0045178 C:basal part of cell IDA:FlyBase.
GO:0005938 C:cell cortex IDA:FlyBase.
GO:0030139 C:endocytic vesicle IDA:UniProtKB.
GO:0045172 C:germline ring canal IDA:FlyBase.
GO:0005875 C:microtubule associated complex IDA:UniProtKB.
GO:0031476 C:myosin VI complex IPI:FlyBase.
GO:0070864 C:sperm individualization complex IDA:FlyBase.
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0042623 F:ATPase activity, coupled NAS:UniProtKB.
GO:0000146 F:microfilament motor activity NAS:UniProtKB.
GO:0007015 P:actin filament organization IMP:FlyBase.
GO:0030048 P:actin filament-based movement NAS:UniProtKB.
GO:0055059 P:asymmetric neuroblast division TAS:FlyBase.
GO:0045167 P:asymmetric protein localization involved in cell fate determination IMP:FlyBase.
GO:0045175 P:basal protein localization IMP:FlyBase.
GO:0007298 P:border follicle cell migration IMP:FlyBase.
GO:0045217 P:cell-cell junction maintenance TAS:FlyBase.
GO:0016044 P:cellular membrane organization TAS:FlyBase.
GO:0019749 P:cytoskeleton-dependent cytoplasmic transport, nurse cell to oocyte IMP:FlyBase.
GO:0007391 P:dorsal closure IEP:UniProtKB.
GO:0040001 P:establishment of mitotic spindle localization IMP:FlyBase.
GO:0007560 P:imaginal disc morphogenesis IMP:FlyBase.
GO:0008363 P:larval chitin-based cuticle development IMP:FlyBase.
GO:0047497 P:mitochondrion transport along microtubule IDA:FlyBase.
GO:0016333 P:morphogenesis of follicular epithelium IMP:FlyBase.
GO:0051647 P:nucleus localization IMP:FlyBase.
GO:0006997 P:nucleus organization IMP:FlyBase.
GO:0045921 P:positive regulation of exocytosis IMP:FlyBase.
GO:0030589 P:pseudocleavage involved in syncytial blastoderm formation IMP:FlyBase.
GO:0032956 P:regulation of actin cytoskeleton organization IMP:FlyBase.
GO:0032880 P:regulation of protein localization IMP:FlyBase.
GO:0007291 P:sperm individualization IMP:FlyBase.
GO:0030317 P:sperm motility IMP:FlyBase.
GO:0007051 P:spindle organization IMP:FlyBase.
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Interpro
IPR000048;    IQ_motif_EF-hand-BS.
IPR001609;    Myosin_head_motor_dom.
IPR004009;    Myosin_N.
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Pfam
PF00063;    Myosin_head;    1.
PF02736;    Myosin_N;    1.
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SMART
SM00015;    IQ;    1.
SM00242;    MYSc;    1.
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PROSITE
PS50096;    IQ;    1.
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PRINTS
PR00193;    MYOSINHEAVY.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   1253       Myosin heavy chain 95F.
                             /FTId=PRO_0000123389.
DOMAIN        1    807       Myosin head-like.
DOMAIN      808    837       IQ.
NP_BIND     151    158       ATP.
REGION      647    666       Actin-binding.
REGION     1187   1193       Hydrophobic region.
COILED      900   1022       Potential.
VAR_SEQ    1047   1047       R -> SFSQVVSNIASRYLNK (in isoform H).
                             /FTId=VSP_003343.
VAR_SEQ    1048   1073       SENVRAQQQALGKQKYDLSKWKYSEL -> YSTLYELPMST
                             TLVNFVNLFLLSQKH (in isoform I).
                             /FTId=VSP_003344.
VAR_SEQ    1074   1253       Missing (in isoform I).
                             /FTId=VSP_003345.
CONFLICT    220    220       V -> M (in Ref. 1; CAA47462).
CONFLICT   1051   1051       V -> L (in Ref. 1; CAA47462).
CONFLICT   1121   1121       R -> P (in Ref. 1; CAA47462).
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Nucleotide Sequence
Length: 4280 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 1253 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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