SG00000206

SpermatogenesisOnline 1.0

Tag

Content

SG ID

SG00000206

UniProt Accession

Q24401_DROME;Q24401;

Theoretical PI

FRAGMENT

Molecular Weight

9757 Da

Genbank Nucleotide ID

Z46790;

Genbank Protein ID

CAA86754.1;

Gene Name

Mst35Ba

Gene Synonyms/Alias

ORFNames=CG4479

Protein Name

Protein Synonyms/Alias

SubName: Male germ-line specific transcript encoding putative protamineFlags: Fragment

Organism

Drosophila melanogaster (Fruit fly)

NCBI Taxonomy ID

7227

Chromosome Location

chr:2L;14879045-14881652;-1

View in Ensembl genome browser

Function in Stage

postmeiotic

Function in Cell Type

spermatid

Description

Temporarily unavailable

The information of related literatures

1. C. Rathke, B. Barckmann, S. Burkhard, S. Jayaramaiah-Raja, J. Roote and R. Renkawitz-Pohl (2010) Distinct functions of Mst77F and protamines in nuclear shaping and chromatin condensation during Drosophila spermiogenesis. Eur J Cell Biol 89(4): 326-38.

Abstract
Chromatin reorganisation is a major event towards the end of mammalian and Drosophila spermatogenesis. In Drosophila, we previously identified protamine A, protamine B and Mst77F as major chromatin components of the mature sperm. Here, an antibody against Mst77F reveals a dual expression pattern of Mst77F as a chromatin component and in association with microtubules during nuclear shaping. Spermatids of ms(3)nc3 (Mst77F(1)) mutants show disturbed nuclear shaping, instability of perinuclear microtubules but no obvious chromatin condensation defects. Furthermore, we generated a deletion including both protamine genes (prot Delta) and observed that in Drosophila, protamine genes are not haploinsufficient in contrast to those of mice and humans. Moreover, we show that in prot Delta mutants, histone degradation, distribution of DNA breaks and Tpl(94D)-eGFP and Mst77F expression proceed as in wild-type males. Surprisingly, in homozygous prot Delta mutants, males are fertile and sperm are motile, while about 20% of sperm show abnormally shaped nuclei. The latter phenotype can be rescued by supplying protamine-eGFP but not by supplying Mst77F-eGFP. Finally, we demonstrate a 21% increase in X-ray-induced mutation rate of prot Delta sperm. These data support the long-standing hypothesis that the switch from a histone- to protamine-based chromatin protects the paternal genome from mutagens. PMID: [20138392]

Figures for illustrating the function of this protein/gene

	Ref: C. Rathke, B. Barckmann, S. Burkhard, S. Jayaramaiah-Raja, J. Roote and R. Renkawitz-Pohl (2010) Distinct functions of Mst77F and protamines in nuclear shaping and chromatin condensation during Drosophila spermiogenesis. Eur J Cell Biol 89(4): 326-38. PMID: [20138392]
	Ref: C. Rathke, B. Barckmann, S. Burkhard, S. Jayaramaiah-Raja, J. Roote and R. Renkawitz-Pohl (2010) Distinct functions of Mst77F and protamines in nuclear shaping and chromatin condensation during Drosophila spermiogenesis. Eur J Cell Biol 89(4): 326-38. PMID: [20138392]
	Ref: C. Rathke, B. Barckmann, S. Burkhard, S. Jayaramaiah-Raja, J. Roote and R. Renkawitz-Pohl (2010) Distinct functions of Mst77F and protamines in nuclear shaping and chromatin condensation during Drosophila spermiogenesis. Eur J Cell Biol 89(4): 326-38. PMID: [20138392]
	Ref: C. Rathke, B. Barckmann, S. Burkhard, S. Jayaramaiah-Raja, J. Roote and R. Renkawitz-Pohl (2010) Distinct functions of Mst77F and protamines in nuclear shaping and chromatin condensation during Drosophila spermiogenesis. Eur J Cell Biol 89(4): 326-38. PMID: [20138392]