SG ID

SG00000216 

UniProt Accession

Q95T18_DROME;Q95T18;  

Theoretical PI

7.09  

Molecular Weight

30000 Da  

Genbank Nucleotide ID

AY060376;  

Genbank Protein ID

AAL25415.1; 

Gene Name

mia 

Gene Synonyms/Alias

Taf60-2, Taf6L 

Protein Name

 

Protein Synonyms/Alias

SubName: LD25013p 

Drosophila melanogaster (Fruit fly) 

7227 

Temporarily unavailable 

The information of related literatures

1. C. E. Metcalf and D. A. Wassarman (2007) Nucleolar colocalization of TAF1 and testis-specific TAFs during Drosophila spermatogenesis. Dev Dyn 236(10): 2836-43. 

Abstract
In Drosophila, testis-specific TBP-associated factors (tTAFs) predominantly localize to spermatocyte nucleoli and regulate the transcription of genes necessary for spermatocyte entry into meiosis. tTAFs are paralogs of generally expressed TAF subunits of transcription factor IID (TFIID). Our recent observation that the generally expressed TAF1 isoform TAF1-2 is greatly enriched in testes prompted us to explore the functional relationship between general TAFs and tTAFs during spermatogenesis. Analysis by immunofluorescence microscopy revealed that among the general TFIID subunits examined (TATA-box binding protein [TBP], TAF1, TAF4, TAF5, and TAF9), only TAF1 colocalized with the tTAF Mia in spermatocyte nucleoli. Nucleolar localization of TAF1, but not Mia, was disrupted in tTAF mutant flies, and TAF1 dissociated from DNA prior to Mia as spermatocytes entered meiosis. Taken together, our results suggest stepwise assembly of a testis-specific TFIID complex (tTFIID) whereby a TAF1 isoform, presumably TAF1-2, is recruited to a core subassembly of tTAFs in spermatocyte nucleoli. PMID: [17823958] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0001673	C:male germ cell nucleus	IDA:FlyBase.
GO:0005730	C:nucleolus	IDA:FlyBase.
GO:0005669	C:transcription factor TFIID complex	ISS:FlyBase.
GO:0007141	P:male meiosis I	IMP:FlyBase.
GO:0010468	P:regulation of gene expression	IMP:FlyBase.
GO:0007286	P:spermatid development	TAS:FlyBase.
GO:0006367	P:transcription initiation from RNA polymerase II promoter	ISS:FlyBase.

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 2288 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 260 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

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Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

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