SG ID

SG00000222 

UniProt Accession

O96828_DROME;O96828;  

Theoretical PI

8.74  

Molecular Weight

84180 Da  

Genbank Nucleotide ID

AL031863;  

Genbank Protein ID

CAA21318.1; 

Gene Name

Psi 

Gene Synonyms/Alias

ec, EG:EG0003.2 

Protein Name

 

Protein Synonyms/Alias

SubName: EG:EG0003.2 protein (Fbgn0014870psi protein); 

Drosophila melanogaster (Fruit fly) 

7227 

Temporarily unavailable 

The information of related literatures

1. E. Labourier, M. Blanchette, J. W. Feiger, M. D. Adams and D. C. Rio (2002) The KH-type RNA-binding protein PSI is required for Drosophila viability, male fertility, and cellular mRNA processing. Genes Dev 16(1): 72-84. 

Abstract
Direct interactions between RNA-binding proteins and snRNP particles modulate eukaryotic pre-mRNA processing patterns to control gene expression. Here, we report that the conserved U1 snRNP-interacting RNA-binding protein PSI is essential for Drosophila viability. A null PSI mutation is recessive lethal at the first-instar larval stage, and lethality is fully rescued by transgenes expressing the PSI protein. A mutant transgene that lacks the PSI-U1 snRNP-interaction domain restores viability but shows courtship behavior abnormalities and meiosis defects during spermatogenesis, resulting in a complete male sterility phenotype. Using cDNA microarrays, we have identified specific target mRNAs with altered expression profiles in these mutant males. A subset of these transcripts is also found associated with PSI in endogenous immunopurified ribonucleoprotein complexes. One specific target, the hrp40/squid transcript, shows an altered pre-mRNA splicing pattern in PSI mutant testes. We conclude that a functional association between the PSI protein and the spliceosomal U1 snRNP particle is required for normal Drosophila development and for the processing of specific PSI-interacting cellular transcripts. These results also validate the use of cDNA microarrays to characterize in vivo RNA-processing defects and alternative pre-mRNA splicing patterns. PMID: [11782446] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005634	C:nucleus	IDA:FlyBase.
GO:0003729	F:mRNA binding	IDA:FlyBase.
GO:0006397	P:mRNA processing	IDA:FlyBase.
GO:0048025	P:negative regulation of nuclear mRNA splicing, via spliceosome	IDA:FlyBase.
GO:0000381	P:regulation of alternative nuclear mRNA splicing, via spliceosome	IMP:FlyBase.
GO:0007283	P:spermatogenesis	IMP:FlyBase.

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Interpro

IPR015096;    DUF1897.
IPR004087;    KH_dom.
IPR004088;    KH_dom_type_1.
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Pfam

PF09005;    DUF1897;    2.
PF00013;    KH_1;    4.
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SMART

SM00322;    KH;    4.
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PROSITE

PS50084;    KH_TYPE_1;    4.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 37688 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 806 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

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