SG ID

SG00000224 

UniProt Accession

Q8T6I0_DROME;Q8T6I0;Q9VG34;  

Theoretical PI

6.06  

Molecular Weight

61005 Da  

Genbank Nucleotide ID

AE014297; AF473822;  

Genbank Protein ID

AAF54856.2; AAL85325.1; 

Gene Name

Past1 

Gene Synonyms/Alias

EHD 

Protein Name

 

Protein Synonyms/Alias

SubName: EH domain containing proteinSubName: Putative achaete scute target 1, isoform B 

Drosophila melanogaster (Fruit fly) 

7227 

Temporarily unavailable 

The information of related literatures

1. Y. Olswang-Kutz, Y. Gertel, S. Benjamin, O. Sela, O. Pekar, E. Arama, H. Steller, M. Horowitz and D. Segal (2009) Drosophila Past1 is involved in endocytosis and is required for germline development and survival of the adult fly. J Cell Sci 122(Pt 4): 471-80. 

Abstract
Endocytosis, which is a key process in eukaryotic cells, has a central role in maintaining cellular homeostasis, nutrient uptake, development and downregulation of signal transduction. This complex process depends on several protein-protein interactions mediated by specific modules. One such module is the EH domain. The EH-domain-containing proteins comprise a family that includes four vertebrate members (EHD1-EHD4) and one Drosophila ortholog, Past1. We used Drosophila as a model to understand the physiological role of this family of proteins. We observed that the two predicted Past1 transcripts are differentially expressed both temporally and spatially during the life cycle of the fly. Endogenous Past1 as well as Past1A and Past1B, expressed from plasmids, were localized mainly to the membrane of Drosophila-derived cells. We generated mutants in the Past1 gene by excising a P-element inserted in it. The Past1 mutants reached adulthood but died precociously. They were temperature sensitive and infertile because of lesions in the reproductive system. Garland cells that originated from Past1 mutants exhibited a marked decrease in their ability to endocytose fluorescently labeled avidin. Genetic interaction was found between Past1 and members of the Notch signaling pathway, suggesting a role for Past1 in this developmentally crucial signaling pathway. PMID: [19174465] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005509	F:calcium ion binding	IEA:InterPro.
GO:0005525	F:GTP binding	IEA:InterPro.
GO:0003924	F:GTPase activity	IEA:InterPro.
GO:0006897	P:endocytosis	IMP:FlyBase.
GO:0006184	P:GTP catabolic process	IEA:GOC.
GO:0008587	P:imaginal disc-derived wing margin morphogenesis	IGI:FlyBase.
GO:0007474	P:imaginal disc-derived wing vein specification	IGI:FlyBase.
GO:0048477	P:oogenesis	IMP:FlyBase.
GO:0007291	P:sperm individualization	IMP:FlyBase.

Back to Top

Interpro

IPR001401;    Dynamin_GTPase.
IPR011992;    EF-hand-like_dom.
IPR018247;    EF_Hand_1_Ca_BS.
IPR018249;    EF_HAND_2.
IPR000261;    EPS15_homology.
Back to Top

Pfam

PF00350;    Dynamin_N;    1.
Back to Top

SMART

SM00027;    EH;    1.
Back to Top

PROSITE

PS00018;    EF_HAND_1;    1.
PS50222;    EF_HAND_2;    1.
PS50031;    EH;    1.
Back to Top

PRINTS

Back to Top

Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 27905053 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 534 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

Comments