SG ID

SG00000230 

UniProt Accession

E1JJR3_DROME;E1JJR3;  

Theoretical PI

6.58  

Molecular Weight

184624 Da  

Genbank Nucleotide ID

AE014298;  

Genbank Protein ID

ACZ95330.1; 

Gene Name

pcm 

Gene Synonyms/Alias

ORFNames=Dmel_CG3291 

Protein Name

 

Protein Synonyms/Alias

SubName: Pacman, isoform BEC=3.1.13.- 

Drosophila melanogaster (Fruit fly) 

7227 

Temporarily unavailable 

The information of related literatures

1.  

Abstract
PMID: [18652575] 

2. M. V. Zabolotskaya, D. P. Grima, M. D. Lin, T. B. Chou and S. F. Newbury (2008) The 5'-3' exoribonuclease Pacman is required for normal male fertility and is dynamically localized in cytoplasmic particles in Drosophila testis cells. Biochem J 416(3): 327-35. 

Abstract
The exoribonuclease Xrn1 is widely recognised as a key component in the 5'-3' RNA degradation pathway. This enzyme is highly conserved between yeast and humans and is known to be involved in RNA interference and degradation of microRNAs as well as RNA turnover. In yeast and human tissue culture cells, Xrn1 has been shown to be a component of P-bodies (processing bodies), dynamic cytoplasmic granules where RNA degradation can take place. In this paper we show for the first time that Pacman, the Drosophila homologue of Xrn1, is localized in cytoplasmic particles in Drosophila testis cells. These particles are present in both the mitotically dividing spermatogonia derived from primordial stem cells and in the transcriptionally active spermatocytes. Pacman is co-localized with the decapping activator dDcp1 and the helicase Me31B (a Dhh1 homologue) in these particles, although this co-localization is not completely overlapping, suggesting that there are different compartments within these granules. Particles containing Pacman respond to stress and depletion of 5'-3' decay factors in the same way as yeast P-bodies, and therefore are likely to be sites of mRNA degradation or storage. Pacman is shown to be required for normal Drosophila spermatogenesis, suggesting that control of mRNA stability is crucial in the testis differentiation pathway. PMID: [18652574] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0000932	C:cytoplasmic mRNA processing body	IDA:FlyBase.
GO:0008409	F:5'-3' exonuclease activity	IEA:InterPro.
GO:0003676	F:nucleic acid binding	IEA:InterPro.
GO:0007391	P:dorsal closure	IMP:FlyBase.
GO:0046529	P:imaginal disc fusion, thorax closure	IMP:FlyBase.
GO:0007476	P:imaginal disc-derived wing morphogenesis	IMP:FlyBase.
GO:0034428	P:nuclear-transcribed mRNA catabolic process, exonucleolytic, 5'-3'	IMP:FlyBase.
GO:0000184	P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay	IMP:FlyBase.
GO:0010603	P:regulation of cytoplasmic mRNA processing body assembly	IMP:FlyBase.
GO:0016246	P:RNA interference	IMP:FlyBase.
GO:0007283	P:spermatogenesis	IMP:FlyBase.
GO:0042060	P:wound healing	IMP:FlyBase.

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Interpro

IPR016494;    5_3_exoribonuclease_1.
IPR004859;    Put_53exo.
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Pfam

PF03159;    XRN_N;    1.
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SMART

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PROSITE

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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 1613 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
Q9W3J9_DROME	CG2116	BioGRID

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

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