Tag Content
SG ID
SG00000233 
UniProt Accession
Theoretical PI
5.35  
Molecular Weight
66326 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Elf 
Gene Synonyms/Alias
Dsup35 
Protein Name
 
Protein Synonyms/Alias
SubName: Elongation factor 1 alpha-like factor 
Organism
Drosophila melanogaster (Fruit fly) 
NCBI Taxonomy ID
7227 
Chromosome Location
chr:2L;12434432-12440605;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. J. Basu, B. C. Williams, Z. Li, E. V. Williams and M. L. Goldberg (1998) Depletion of a Drosophila homolog of yeast Sup35p disrupts spindle assembly, chromosome segregation, and cytokinesis during male meiosis. Cell Motil Cytoskeleton 39(4): 286-302. 

Abstract
In the course of a genetic screen for male-sterile mutations in Drosophila affecting chromosome segregation during the meiotic divisions in spermatocytes, we identified the mutation dsup35(63D). Examination of mutant testes showed that chromosome misbehavior was a consequence of major disruptions in meiotic spindle assembly. These perturbations included problems in aster formation, separation, and migration around the nuclear envelope; aberrations in spindle organization and integrity; and disappearance of the ana/telophase central spindle, which in turn disrupts cytokinesis. The dsup35(63D) mutation is caused by a P element insertion that affects, specifically in the testis, the expression of a gene (dsup35) encoding the Drosophila homolog of the yeast Sup35p and Xenopus eRF3 proteins. These proteins are involved in the termination of polypeptide synthesis on ribosomes, but previous studies have suggested that Sup35p and closely related proteins of the same family also interact directly with microtubules. An affinity-purified antibody directed against the product of the dsup35 gene was prepared; interestingly, this antibody specifically labels primary spermatocytes in one or two discrete foci of unknown structure within the nucleoplasm. We discuss how depletion of the dsup35 gene product in spermatocytes might lead to the global disruptions in meiotic spindle assembly seen in mutant spermatocytes. PMID: [9556329] 

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Figures for illustrating the function of this protein/gene
Function
 
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Subcellular Location
 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005829 C:cytosol ISS:FlyBase.
GO:0005525 F:GTP binding ISS:FlyBase.
GO:0003924 F:GTPase activity NAS:FlyBase.
GO:0003746 F:translation elongation factor activity IEA:UniProtKB-KW.
GO:0003747 F:translation release factor activity NAS:FlyBase.
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Interpro
IPR009818;    Ataxin-2_C.
IPR000795;    ProtSyn_GTP-bd.
IPR009001;    Transl_elong_EF1A/Init_IF2_C.
IPR004161;    Transl_elong_EFTu/EF1A_2.
IPR004160;    Transl_elong_EFTu/EF1A_C.
IPR009000;    Transl_elong_init/rib_B-barrel.
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Pfam
PF00009;    GTP_EFTU;    1.
PF03144;    GTP_EFTU_D2;    1.
PF03143;    GTP_EFTU_D3;    1.
PF07145;    PAM2;    1.
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SMART
PROSITE
PS00301;    EFACTOR_GTP;    1.
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PRINTS
PR00315;    ELONGATNFCT.;   
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Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
Nucleotide Sequence
Length: 3096 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 619 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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