SG ID

SG00000235 

UniProt Accession

O18397_DROME;O18397;  

Theoretical PI

5.58  

Molecular Weight

89235 Da  

Genbank Nucleotide ID

Y13951;  

Genbank Protein ID

CAA74289.1; 

Gene Name

Osbp 

Gene Synonyms/Alias

ORFNames=CG6708 

Protein Name

Oxysterol-binding protein 

Protein Synonyms/Alias

 

Drosophila melanogaster (Fruit fly) 

7227 

Temporarily unavailable 

The information of related literatures

1. Z. Ma, Z. Liu and X. Huang (2010) OSBP- and FAN-mediated sterol requirement for spermatogenesis in Drosophila. Development 137(22): 3775-84. 

Abstract
Members of the oxysterol binding protein (OSBP) family are involved in diverse biological processes, including non-vesicular sterol transport and vesicle trafficking. The mechanisms by which OSBPs integrate functionally with developmental and physiological processes remain elusive. Here, we report the in vivo analysis of OSBP function in the model organism Drosophila. Osbp mutants are male-sterile and exhibit defects in individualization, the process by which each spermatid is packaged into its own membrane. Overexpression of OSBP leads to post-eclosion behaviour defects that can be suppressed by co-expression of endoplasmic reticulum-specific VAP family proteins. Most notably, FAN, a testis-specific VAP protein, acts together with OSBP genetically and physically to regulate the individualization process. OSBP-positive and sterol-enriched speckles are found at the leading edge of the individualization complex in wild type but not in Osbp or fan mutants, suggesting that sterol trafficking might play key roles during the membrane-remodelling phase of individualization. In addition, Osbp mutants that are fed additional sterols partially recover fertility, implying that male sterility is attributable to sterol shortage. Thus, we have identified an OSBP- and FAN-mediated sterol requirement in Drosophila spermatogenesis. PMID: [20943709] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005783	C:endoplasmic reticulum	IDA:FlyBase.
GO:0005794	C:Golgi apparatus	IDA:FlyBase.
GO:0005543	F:phospholipid binding	IEA:InterPro.
GO:0007030	P:Golgi organization	IMP:FlyBase.
GO:0006886	P:intracellular protein transport	IMP:FlyBase.
GO:0006869	P:lipid transport	IEA:UniProtKB-KW.
GO:0007291	P:sperm individualization	IMP:FlyBase.

Back to Top

Interpro

IPR000648;    Oxysterol-bd.
IPR018494;    Oxysterol-bd_CS.
IPR011993;    PH_like_dom.
IPR001849;    Pleckstrin_homology.
Back to Top

Pfam

PF01237;    Oxysterol_BP;    1.
PF00169;    PH;    1.
Back to Top

SMART

SM00233;    PH;    1.
Back to Top

PROSITE

PS01013;    OSBP;    1.
PS50003;    PH_DOMAIN;    1.
Back to Top

PRINTS

Back to Top

Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 3247 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 784 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
Q8SZF1_DROME	spn-B	IntAct
Q9XYZ4_DROME	mre11	IntAct

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

Comments