SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000243 
UniProt Accession
Theoretical PI
5.02  
Molecular Weight
12479 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Dlc90F 
Gene Synonyms/Alias
Tctex 
Protein Name
Dynein light chain Tctex-type 
Protein Synonyms/Alias
TCTEX-1 protein homolog; 
Organism
Drosophila melanogaster (Fruit fly) 
NCBI Taxonomy ID
7227 
Chromosome Location
chr:3R;14124927-14125764;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. M. G. Li, M. Serr, E. A. Newman and T. S. Hays (2004) The Drosophila tctex-1 light chain is dispensable for essential cytoplasmic dynein functions but is required during spermatid differentiation. Mol Biol Cell 15(7): 3005-14. 

Abstract
Variations in subunit composition and modification have been proposed to regulate the multiple functions of cytoplasmic dynein. Here, we examine the role of the Drosophila ortholog of tctex-1, the 14-kDa dynein light chain. We show that the 14-kDa light chain is a bona fide component of Drosophila cytoplasmic dynein and use P element excision to generate flies that completely lack this dynein subunit. Remarkably, the null mutant is viable and the only observed defect is complete male sterility. During spermatid differentiation, the 14-kDa light chain is required for the localization of a nuclear "cap" of cytoplasmic dynein and for proper attachment between the sperm nucleus and flagellar basal body. Our results provide evidence that the function of the 14-kDa light chain in Drosophila is distinct from other dynein subunits and is not required for any essential functions in early development or in the adult organism. PMID: [15090621] 

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Figures for illustrating the function of this protein/gene
Ref: M. G. Li, M. Serr, E. A. Newman and T. S. Hays (2004) The Drosophila tctex-1 light chain is dispensable for essential cytoplasmic dynein functions but is required during spermatid differentiation. Mol Biol Cell 15(7): 3005-14. PMID: [15090621]
Ref: M. G. Li, M. Serr, E. A. Newman and T. S. Hays (2004) The Drosophila tctex-1 light chain is dispensable for essential cytoplasmic dynein functions but is required during spermatid differentiation. Mol Biol Cell 15(7): 3005-14. PMID: [15090621]
Ref: M. G. Li, M. Serr, E. A. Newman and T. S. Hays (2004) The Drosophila tctex-1 light chain is dispensable for essential cytoplasmic dynein functions but is required during spermatid differentiation. Mol Biol Cell 15(7): 3005-14. PMID: [15090621]
Function
Acts as one of several non-catalytic accessorycomponents of the cytoplasmic dynein complex that are thought tobe involved in linking dynein to cargos and to adapter proteinsthat regulate dynein function. Cytoplasmic dynein acts as a motorfor the intracellular retrograde motility of vesicles andorganelles along microtubules. Required for spermatiddifferentiation. Is not required for polarized transport inrhabdomere development and appears to be a non-essential componentof the cytoplasmic dynein complex. 
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Subcellular Location
Cytoplasm, cytoskeleton (Probable). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm IEA:UniProtKB-KW.
GO:0030286 C:dynein complex ISS:FlyBase.
GO:0005874 C:microtubule IEA:UniProtKB-KW.
GO:0042623 F:ATPase activity, coupled ISS:FlyBase.
GO:0003774 F:motor activity IEA:UniProtKB-KW.
GO:0007018 P:microtubule-based movement ISS:FlyBase.
GO:0007067 P:mitosis IMP:FlyBase.
GO:0007286 P:spermatid development IMP:FlyBase.
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Interpro
IPR005334;    Tctex.
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Pfam
PF03645;    Tctex-1;    1.
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SMART
PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    111       Dynein light chain Tctex-type.
                             /FTId=PRO_0000195155.
HELIX        14     27
HELIX        34     53
TURN         54     56
STRAND       58     69
STRAND       75     83
TURN         85     87
STRAND       89     96
STRAND       98    110
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Nucleotide Sequence
Length: 551 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 111 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Bsg25DIntAct 
CG30497BioGRID 
CG30497BioGRID 
CG31145BioGRID 
awdBioGRID 
CG31145BioGRID 
Bsg25DBioGRID 
Bsg25D-RCBioGRID 
Fur2BioGRID 
Fur2BioGRID 
enaBioGRID 
Fur2BioGRID 
grkIntAct 
Hsp23BioGRID 
Hsp67BcBioGRID 
metlBioGRID 
CG4802IntAct 
awdBioGRID 
OteBioGRID 
PfrxBioGRID 
UbcD2BioGRID 
CG16786BioGRID 
CG7208BioGRID 
metlIntAct 
Bsg25DBioGRID 
CG32581-RABioGRID 
CG7687BioGRID 
CG1383BioGRID 
CG2267BioGRID 
CG6178BioGRID 
CG10187BioGRID 
CG31122BioGRID 
CG7208BioGRID 
fabpIntAct 
Npc2dBioGRID 
Tctex-1BioGRID 
CG10098MINT 
msb1lMINT 
OliBioGRID 
Bsg25DBioGRID 
CG5565-RABioGRID 
CG13312BioGRID 
CG13022BioGRID 
PfrxBioGRID 
CG8974BioGRID 
CG10990MINT 
_MINT 
rapBioGRID 
UbcD2BioGRID 
enaIntAct 
ewgIntAct 
Fur2IntAct 
grkBioGRID 
Hsp23IntAct 
Hsp67BcIntAct 
awdIntAct 
OteIntAct 
CG16786IntAct 
metlMINT 
CG32581-RAIntAct 
_MINT 
CG2267MINT 
CG6178MINT 
CG10187MINT 
CG31122MINT 
CG7208IntAct 
Npc2dMINT 
Tctex-1IntAct 
CG10098BioGRID 
msb1lBioGRID 
OliMINT 
CG5565-RAMINT 
CG13312MINT 
CG13022MINT 
PfrxIntAct 
CG10990BioGRID 
UbcD2MINT 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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