SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000244 
UniProt Accession
Theoretical PI
6.24  
Molecular Weight
84954 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
fws 
Gene Synonyms/Alias
ORFNames=CG6549 
Protein Name
Conserved oligomeric Golgi complex subunit 5 
Protein Synonyms/Alias
COG complex subunit 5 Component of oligomeric Golgi complex 5; Protein four way stop; 
Organism
Drosophila melanogaster (Fruit fly) 
NCBI Taxonomy ID
7227 
Chromosome Location
chr:2L;17478442-17481212;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. R. M. Farkas, M. G. Giansanti, M. Gatti and M. T. Fuller (2003) The Drosophila Cog5 homologue is required for cytokinesis, cell elongation, and assembly of specialized Golgi architecture during spermatogenesis. Mol Biol Cell 14(1): 190-200. 

Abstract
The multisubunit conserved oligomeric Golgi (COG) complex has been shown previously to be involved in Golgi function in yeast and mammalian tissue culture cells. Despite this broad conservation, several subunits, including Cog5, were not essential for growth and showed only mild effects on secretion when mutated in yeast, raising questions about what functions these COG complex subunits play in the life of the cell. Here, we show that function of the gene four way stop (fws), which encodes the Drosophila Cog5 homologue, is necessary for dramatic changes in cellular and subcellular morphology during spermatogenesis. Loss-of-function mutations in fws caused failure of cleavage furrow ingression in dividing spermatocytes and failure of cell elongation in differentiating spermatids and disrupted the formation and/or stability of the Golgi-based spermatid acroblast. Consistent with the lack of a growth defect in yeast lacking Cog5, animals lacking fws function were viable, although males were sterile. Fws protein localized to Golgi structures throughout spermatogenesis. We propose that Fws may directly or indirectly facilitate efficient vesicle traffic through the Golgi to support rapid and extensive increases in cell surface area during spermatocyte cytokinesis and polarized elongation of differentiating spermatids. Our study suggests that Drosophila spermatogenesis can be an effective sensitized genetic system to uncover in vivo functions for proteins involved in Golgi architecture and/or vesicle transport. PMID: [12529436] 

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Figures for illustrating the function of this protein/gene
Ref: R. M. Farkas, M. G. Giansanti, M. Gatti and M. T. Fuller (2003) The Drosophila Cog5 homologue is required for cytokinesis, cell elongation, and assembly of specialized Golgi architecture during spermatogenesis. Mol Biol Cell 14(1): 190-200. PMID: [12529436]
Ref: R. M. Farkas, M. G. Giansanti, M. Gatti and M. T. Fuller (2003) The Drosophila Cog5 homologue is required for cytokinesis, cell elongation, and assembly of specialized Golgi architecture during spermatogenesis. Mol Biol Cell 14(1): 190-200. PMID: [12529436]
Ref: R. M. Farkas, M. G. Giansanti, M. Gatti and M. T. Fuller (2003) The Drosophila Cog5 homologue is required for cytokinesis, cell elongation, and assembly of specialized Golgi architecture during spermatogenesis. Mol Biol Cell 14(1): 190-200. PMID: [12529436]
Ref: R. M. Farkas, M. G. Giansanti, M. Gatti and M. T. Fuller (2003) The Drosophila Cog5 homologue is required for cytokinesis, cell elongation, and assembly of specialized Golgi architecture during spermatogenesis. Mol Biol Cell 14(1): 190-200. PMID: [12529436]
Ref: R. M. Farkas, M. G. Giansanti, M. Gatti and M. T. Fuller (2003) The Drosophila Cog5 homologue is required for cytokinesis, cell elongation, and assembly of specialized Golgi architecture during spermatogenesis. Mol Biol Cell 14(1): 190-200. PMID: [12529436]
Function
Required for normal Golgi function and necessary duringspermatogenesis. Required for cleavage furrow ingression duringcytokinesis in dividing spermatocytes and for the extensivepolarized cell growth that accompanies spermatid elongation. 
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Subcellular Location
Golgi apparatus membrane; Peripheralmembrane protein. Note=Localized to Golgi membrane throughoutspermatogenesis, as seen in spermatocytes and differentiatingspermatids. 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0044431 C:Golgi apparatus part IDA:UniProtKB.
GO:0000139 C:Golgi membrane IEA:UniProtKB-SubCell.
GO:0000916 P:actomyosin contractile ring contraction IMP:FlyBase.
GO:0016044 P:cellular membrane organization NAS:FlyBase.
GO:0007110 P:cytokinesis after meiosis I IMP:FlyBase.
GO:0007111 P:cytokinesis after meiosis II IMP:FlyBase.
GO:0007112 P:male meiosis cytokinesis IMP:UniProtKB.
GO:0043147 P:meiotic spindle stabilization IMP:FlyBase.
GO:0007275 P:multicellular organismal development IEA:UniProtKB-KW.
GO:0015031 P:protein transport IEA:UniProtKB-KW.
GO:0007290 P:spermatid nucleus elongation IMP:UniProtKB.
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Interpro
IPR019465;    COG_su5.
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Pfam
PF10392;    COG5;    1.
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SMART
PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    751       Conserved oligomeric Golgi complex
                             subunit 5.
                             /FTId=PRO_0000213511.
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Nucleotide Sequence
Length: bp   Go to nucleotide: FASTA
Protein Sequence
Length: 751 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
Comments