SG00000251

SpermatogenesisOnline 1.0

Tag

Content

SG ID

SG00000251

UniProt Accession

PLK4_DROME;O97143;Q86NL8;

Theoretical PI

8.83

Molecular Weight

85887 Da

Genbank Nucleotide ID

AF106952; AE014296; BT004846; BT044561;

Genbank Protein ID

AAD19607.1; AAF51737.1; AAO45202.1; ACI15756.1;

Gene Name

SAK

Gene Synonyms/Alias

ORFNames=CG7186

Protein Name

Serine/threonine-protein kinase PLK4

Protein Synonyms/Alias

EC=2.7.11.21 Polo-like kinase 4;PLK-4 Serine/threonine-protein kinase SAK;

Organism

Drosophila melanogaster (Fruit fly)

NCBI Taxonomy ID

7227

Chromosome Location

chr:3L;21505241-21508984;-1

View in Ensembl genome browser

Function in Stage

postmeiotic

meiotic

Function in Cell Type

spermatocyte

spermatid

Description

Temporarily unavailable

The information of related literatures

1. M. Bettencourt-Dias, A. Rodrigues-Martins, L. Carpenter, M. Riparbelli, L. Lehmann, M. K. Gatt, N. Carmo, F. Balloux, G. Callaini and D. M. Glover (2005) SAK/PLK4 is required for centriole duplication and flagella development. Curr Biol 15(24): 2199-207.

Abstract
BACKGROUND PMID: [16326102]

Figures for illustrating the function of this protein/gene

Function

Serine/threonine-protein kinase that plays a centralrole in centriole duplication. Able to trigger procentrioleformation on the surface of the mother centriole cylinder, usingmother centriole as a platform, leading to the recruitment ofcentriole biogenesis proteins such as sas-6. When overexpressed,it is able to induce centrosome amplification through thesimultaneous generation of multiple procentrioles adjoining eachparental centriole during S phase. Centrosome amplificationfollowing overexpression can initiate tumorigenesis, highlightingthe importance of centrosome regulation in cancers.

Subcellular Location

Cytoplasm, cytoskeleton, centrosome,centriole.

Tissue Specificity

Gene Ontology

GO ID	GO term	Evidence
GO:0005814	C:centriole	IDA:FlyBase.
GO:0005524	F:ATP binding	IEA:UniProtKB-KW.
GO:0004672	F:protein kinase activity	NAS:FlyBase.
GO:0004674	F:protein serine/threonine kinase activity	IEA:UniProtKB-KW.
GO:0004713	F:protein tyrosine kinase activity	IEA:InterPro.
GO:0007099	P:centriole replication	IMP:FlyBase.
GO:0007052	P:mitotic spindle organization	IMP:FlyBase.
GO:0046599	P:regulation of centriole replication	IDA:FlyBase.
GO:0007288	P:sperm axoneme assembly	IMP:FlyBase.

Interpro

IPR011009;    Kinase-like_dom.
IPR000959;    POLO_box_duplicated_dom.
IPR000719;    Prot_kinase_cat_dom.
IPR017441;    Protein_kinase_ATP_BS.
IPR002290;    Ser/Thr_dual-sp_kinase_dom.
IPR008266;    Tyr_kinase_AS.
Back to Top

Pfam

PF00069; Pkinase; 1.
Back to Top

SMART

SM00220; S_TKc; 1.
Back to Top

PROSITE

PS50078;    POLO_BOX;    1.
PS00107;    PROTEIN_KINASE_ATP;    1.
PS50011;    PROTEIN_KINASE_DOM;    1.
PS00108;    PROTEIN_KINASE_ST;    FALSE_NEG.
Back to Top

PRINTS

Created Date

18-Oct-2012

Record Type

Experiment identified

Protein sequence Annotation

CHAIN         1    769       Serine/threonine-protein kinase PLK4.
                             /FTId=PRO_0000385291.
DOMAIN       14    267       Protein kinase.
DOMAIN      665    734       POLO box.
NP_BIND      20     28       ATP (By similarity).
ACT_SITE    138    138       Proton acceptor (By similarity).
BINDING      43     43       ATP (By similarity).
MUTAGEN     293    293       S->A: In SAK-ND; impairs interaction with
                             SCF(Slimb) ubiquitin ligase complex and
                             subsequent ubiquitination; when
                             associated with A-297.
MUTAGEN     297    297       T->A: In SAK-ND; impairs interaction with
                             SCF(Slimb) ubiquitin ligase complex and
                             subsequent ubiquitination; when
                             associated with A-292.
CONFLICT    145    145       L -> V (in Ref. 4; AAO45202).
Back to Top

Nucleotide Sequence

Length: 2574 bp Go to nucleotide: FASTA

Protein Sequence

Length: 769 bp Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
A1Z6E0_DROME	gus	IntAct
A1Z743_DROME	CG30497	IntAct
A1Z927_DROME	Sin3A	IntAct
ATPA_DROME	blw	IntAct
BCD_DROME	bcd	IntAct
CCNC_DROME	CycC	IntAct
CEG1A_DROME	cenG1A	IntAct
E2QCP0_DROME	His3.3A	IntAct
ECR_DROME	EcR	IntAct
ENA_DROME	ena	IntAct
FRY_DROME	fry	IntAct
FTF1B_DROME	Hr39	IntAct
MED15_DROME	MED15	IntAct
O46070_DROME	EG:25E8.4	IntAct
O76136_DROME	PHDP	IntAct
PANG1_DROME	pan	IntAct
PGAM5_DROME	Pgam5	IntAct
A1Z6D7_DROME	gus	BioGRID
A1Z6D9_DROME	gus	BioGRID
A1Z6E0_DROME	gus	BioGRID
A1Z6E2_DROME	gus	BioGRID
A1Z6E8_DROME	CG10395	BioGRID
A1Z6G4_DROME	CG17508	BioGRID
A1Z743_DROME	CG30497	BioGRID
A1Z745_DROME	CG30497	BioGRID
A1Z7M3_DROME	CG34350	BioGRID
A1Z927_DROME	Sin3A	BioGRID
A1Z928_DROME	Sin3A	BioGRID
A4UZ51_DROME	EcR	BioGRID
A8JNJ2_DROME	CG11486	BioGRID
ATPA_DROME	blw	BioGRID
B5RJS3_DROME	pan-RG	BioGRID
B7FNK5_DROME	cenG1A-RC	BioGRID
B7Z143_DROME	CG11138	BioGRID
BCD_DROME	bcd	BioGRID
C6SV45_DROME	spn-A-RA	BioGRID
CCNC_DROME	CycC	BioGRID
CEG1A_DROME	cenG1A	BioGRID
DIMM_DROME	dimm	BioGRID
E1JGY2_DROME	EcR	BioGRID
E2QCP0_DROME	His3.3A	BioGRID
E2QCY4_DROME	fry	BioGRID
ECR_DROME	EcR	BioGRID
ENA_DROME	ena	BioGRID
FRY_DROME	fry	BioGRID
FTF1B_DROME	Hr39	BioGRID
H33_DROME	His3.3A	BioGRID
MED15_DROME	MED15	BioGRID
O46070_DROME	EG:25E8.4	BioGRID
O76136_DROME	PHDP	MINT
PANG1_DROME	pan	BioGRID
PANG2_DROME	pan	BioGRID
PGAM5_DROME	Pgam5	BioGRID
PPT2_DROME	Ppt2	BioGRID
Q0IGR9	dimm	MINT
Q5U0Y0_DROME	Sin3A	BioGRID
Q7JUY1_DROME	gus	BioGRID
Q7K0V7_DROME	CG8272	BioGRID
Q7K306_DROME	Cdk4	BioGRID
Q7KV83_DROME	CG11486-RK	BioGRID
Q7PLE8_DROME	Rab21	BioGRID
Q7PLE9_DROME	Rab21	BioGRID
Q7Z2C5_DROME	CG12723	BioGRID
Q8IMG7_DROME	CycG	BioGRID
Q8IRE7_DROME	CG11486	BioGRID
Q8MLL8	_	MINT
Q8MRG8_DROME	Rab21	MINT
Q8MS22_DROME	CG11138	MINT
Q8MSW5_DROME	CG5003	MINT
Q8SXL5_DROME	CG13806	BioGRID
Q8SXN9_DROME	CG1383	BioGRID
Q8SYU4_DROME	CG17508	MINT
Q8SZP2_DROME	TwdlE	BioGRID
Q8T965_DROME	CG15676	BioGRID
Q8T9L6_DROME	CG13900	BioGRID
Q95RL9_DROME	CG10395	MINT
Q95RR8_DROME	CG11486	BioGRID
Q95TJ9_DROME	CycG	BioGRID
Q961D1_DROME	CycK	BioGRID
Q9I7L0_DROME	CG18745	BioGRID
Q9I7T8_DROME	CG11486-RB	BioGRID
Q9I7Z8_DROME	CG18273	BioGRID
Q9V4R8	_	MINT
Q9V4Y8	_	MINT
Q9V514	_	MINT
Q9V7R5	_	MINT
Q9V9W0	_	MINT
Q9VAZ0_DROME	CG5003-RA	BioGRID
Q9VCW3_DROME	Nup133	IntAct
Q9VFQ3_DROME	E5	BioGRID
Q9VG22_DROME	d-cup	BioGRID
Q9VL21_DROME	CG5708	BioGRID
Q9VL27_DROME	CG5731	BioGRID
Q9VNE1_DROME	rev7	BioGRID
Q9VQ95_DROME	CG31679	BioGRID
Q9VRQ4_DROME	CG5568	BioGRID
Q9VS41_DROME	unc-13-4A	BioGRID
Q9VU23_DROME	CG14119	BioGRID
Q9VUD3_DROME	Sox21b	BioGRID
Q9VWT7_DROME	CG15043	BioGRID
Q9VYF9_DROME	CG12723	MINT
Q9VYL7_DROME	CG11138	BioGRID
Q9VYL8_DROME	CG11138	BioGRID
Q9VZG2_DROME	Cpr64Aa	BioGRID
Q9VZV3_DROME	CG11486	BioGRID
Q9W0M7_DROME	CG13900-RA	BioGRID
Q9W0Y0_DROME	CG15861	BioGRID
Q9W1H7_DROME	PHDP	BioGRID
Q9W1P1_DROME	CG13558	BioGRID
Q9W221_DROME	CG13510-RA	BioGRID
Q9W351_DROME	CG16892-RA	BioGRID
RAD51_DROME	spn-A	BioGRID
VTU2_DROME	Vm26Ab	BioGRID
PPT2_DROME	Ppt2	IntAct
Q0IGR9	dimm	IntAct
Q7K0V7_DROME	CG8272	IntAct
Q7K306_DROME	Cdk4	IntAct
Q8MRG8_DROME	Rab21	IntAct
Q8MS22_DROME	CG11138	IntAct
Q8MSW5_DROME	CG5003	IntAct
Q8SXL5_DROME	CG13806	IntAct
Q8SYU4_DROME	CG17508	IntAct
Q8SZP2_DROME	TwdlE	IntAct
Q8T965_DROME	CG15676	IntAct
Q8T9L6_DROME	CG13900	IntAct
Q95RL9_DROME	CG10395	IntAct
Q961D1_DROME	CycK	IntAct
Q9I7L0_DROME	CG18745	IntAct
Q9I7T8_DROME	CG11486-RB	IntAct
Q9I7Z8_DROME	CG18273	IntAct
Q9VCW3_DROME	Nup133	BioGRID
Q9VFQ3_DROME	E5	IntAct
Q9VG22_DROME	d-cup	IntAct
Q9VL21_DROME	CG5708	IntAct
Q9VL27_DROME	CG5731	IntAct
Q9VNE1_DROME	rev7	IntAct
Q9VRQ4_DROME	CG5568	IntAct
Q9VS41_DROME	unc-13-4A	IntAct
Q9VU23_DROME	CG14119	IntAct
Q9VUD3_DROME	Sox21b	IntAct
Q9VWT7_DROME	CG15043	IntAct
Q9VYF9_DROME	CG12723	IntAct
Q9VZU6_DROME	BtbVII	IntAct
Q9W0Y0_DROME	CG15861	IntAct
Q9W1P1_DROME	CG13558	IntAct
Q9W221_DROME	CG13510-RA	IntAct
RAD51_DROME	spn-A	IntAct
VTU2_DROME	Vm26Ab	IntAct

Other Protein-Protein interaction resources

String database

View Microarray data

Temporarily unavailable

Comments

Click to make comment on this record