Tag Content
SG ID
SG00000253 
UniProt Accession
Theoretical PI
5.3  
Molecular Weight
74980 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Grip75 
Gene Synonyms/Alias
75p 
Protein Name
Gamma-tubulin complex component 4 homolog 
Protein Synonyms/Alias
Gamma-ring complex protein 75 kDa;d75pdGrip75 
Organism
Drosophila melanogaster (Fruit fly) 
NCBI Taxonomy ID
7227 
Chromosome Location
chr:2L;10483285-10485857;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. N. Vogt, I. Koch, H. Schwarz, F. Schnorrer and C. Nusslein-Volhard (2006) The gammaTuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline. Development 133(20): 3963-72. 

Abstract
The gamma-tubulin ring complex (gammaTuRC) forms an essential template for microtubule nucleation in animal cells. The molecular composition of the gammaTuRC has been described; however, the functions of the subunits proposed to form the cap structure remain to be characterized in vivo. In Drosophila, the core components of the gammaTuRC are essential for mitosis, whereas the cap component Grip75 is not required for viability but functions in bicoid RNA localization during oogenesis. The other cap components have not been analyzed in vivo. We report the functional characterization of the cap components Grip128 and Grip75. Animals with mutations in Dgrip128 or Dgrip75 are viable, but both males and females are sterile. Both proteins are required for the formation of distinct sets of microtubules, which facilitate bicoid RNA localization during oogenesis, the formation of the central microtubule aster connecting the meiosis II spindles in oocytes and cytokinesis in male meiosis. Grip75 and Grip128 anchor the axoneme at the nucleus during sperm elongation. We propose that Grip75 and Grip128 are required to tether microtubules at specific microtubule-organizing centers, instead of being required for general microtubule nucleation. The gammaTuRC cap structure may be essential only for non-centrosome-based microtubule functions. PMID: [16971473] 

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Figures for illustrating the function of this protein/gene
Ref: N. Vogt, I. Koch, H. Schwarz, F. Schnorrer and C. Nusslein-Volhard (2006) The gammaTuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline. Development 133(20): 3963-72. PMID: [16971473]
Ref: N. Vogt, I. Koch, H. Schwarz, F. Schnorrer and C. Nusslein-Volhard (2006) The gammaTuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline. Development 133(20): 3963-72. PMID: [16971473]
Ref: N. Vogt, I. Koch, H. Schwarz, F. Schnorrer and C. Nusslein-Volhard (2006) The gammaTuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline. Development 133(20): 3963-72. PMID: [16971473]
Ref: N. Vogt, I. Koch, H. Schwarz, F. Schnorrer and C. Nusslein-Volhard (2006) The gammaTuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline. Development 133(20): 3963-72. PMID: [16971473]
Function
Gamma-tubulin complex is necessary for microtubulenucleation at the centrosome (By similarity). 
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Subcellular Location
Cytoplasm, cytoskeleton, centrosome(Potential). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0008274 C:gamma-tubulin ring complex NAS:FlyBase.
GO:0005874 C:microtubule IEA:UniProtKB-KW.
GO:0000922 C:spindle pole IEA:InterPro.
GO:0005200 F:structural constituent of cytoskeleton NAS:FlyBase.
GO:0051298 P:centrosome duplication IMP:FlyBase.
GO:0007112 P:male meiosis cytokinesis IMP:FlyBase.
GO:0007020 P:microtubule nucleation NAS:FlyBase.
GO:0007067 P:mitosis IMP:FlyBase.
GO:0007052 P:mitotic spindle organization IMP:FlyBase.
GO:0007058 P:spindle assembly involved in female meiosis II IMP:FlyBase.
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Interpro
IPR007259;    Spc97_Spc98.
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Pfam
PF04130;    Spc97_Spc98;    1.
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SMART
PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    650       Gamma-tubulin complex component 4
                             homolog.
                             /FTId=PRO_0000078126.
MOD_RES     214    214       Phosphoserine.
MOD_RES     216    216       Phosphothreonine.
MOD_RES     218    218       Phosphoserine.
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Nucleotide Sequence
Length: bp   Go to nucleotide: FASTA
Protein Sequence
Length: 650 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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