SG ID

SG00000278 

UniProt Accession

Q9VK14_DROME;Q9VK14;  

Theoretical PI

4.89  

Molecular Weight

31903 Da  

Genbank Nucleotide ID

AE014134; AY075285; AY147241;  

Genbank Protein ID

AAF53268.1; AAL68152.1; AAN63095.1; 

Gene Name

GN ORFNames=CG5648, Dmel_CG5648 

Gene Synonyms/Alias

 

Protein Name

Proteasome subunit alpha type 

Protein Synonyms/Alias

EC=3.4.25.1 

Drosophila melanogaster (Fruit fly) 

7227 

Temporarily unavailable 

The information of related literatures

1. L. Zhong and J. M. Belote (2007) The testis-specific proteasome subunit Prosalpha6T of D. melanogaster is required for individualization and nuclear maturation during spermatogenesis. Development 134(19): 3517-25. 

Abstract
Most regulated proteolysis in eukaryotes is carried out by the 26S proteasome. This large, multisubunit complex comprises a catalytic core particle (20S proteasome) and a regulatory particle (19S regulator) capping each end. In Drosophila, about a third of the 32 proteasome subunits are found to have testis-specific isoforms, encoded by paralogous genes. Here, we characterize in detail the spermatogenic expression of the core particle subunit Prosalpha6 (Pros35) and its testis-specific isoform Prosalpha6T. Using GFP-tagged transgenes, it is shown that whereas the Prosalpha6 subunit is expressed in early stages of spermatogenesis, gradually fading away following meiosis, the testis-specific Prosalpha6T becomes prominent in spermatid nuclei and cytoplasm after meiosis, and persists in mature sperm. In addition, these subunits are found in numerous ;speckles' near individualization complexes, similar to the previously described expression pattern of the caspase Dronc (Nedd2-like caspase), suggesting a link to the apoptosis pathway. We also studied the phenotypes of a loss-of-function mutant of Prosalpha6T generated by targeted homologous recombination. Homozygous males are sterile and show spermatogenic defects in sperm individualization and nuclear maturation, consistent with the expression pattern of Prosalpha6T. The results demonstrate a functional role of testis-specific proteasomes during Drosophila spermatogenesis. PMID: [17728345] 

Figures for illustrating the function of this protein/gene

Function

The proteasome is a multicatalytic proteinase complexwhich is characterized by its ability to cleave peptides with Arg,Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral orslightly basic pH. The proteasome has an ATP-dependent proteolyticactivity (By similarity). 

Subcellular Location

Cytoplasm. Nucleus (By similarity). 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005829	C:cytosol	IDA:FlyBase.
GO:0001673	C:male germ cell nucleus	IDA:FlyBase.
GO:0019773	C:proteasome core complex, alpha-subunit complex	IEA:InterPro.
GO:0004298	F:threonine-type endopeptidase activity	IEA:UniProtKB-KW.
GO:0006974	P:response to DNA damage stimulus	IMP:FlyBase.
GO:0007291	P:sperm individualization	IMP:FlyBase.
GO:0007290	P:spermatid nucleus elongation	IMP:FlyBase.
GO:0006511	P:ubiquitin-dependent protein catabolic process	IEA:InterPro.

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Interpro

IPR000426;    Proteasome_asu_N.
IPR023332;    Proteasome_suA-type.
IPR001353;    Proteasome_sua/b.
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Pfam

PF00227;    Proteasome;    1.
PF10584;    Proteasome_A_N;    1.
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SMART

SM00948;    Proteasome_A_N;    1.
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PROSITE

PS00388;    PROTEASOME_A_1;    1.
PS51475;    PROTEASOME_A_2;    1.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 289 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
PRS4_DROME	Pros26.4	IntAct
PSA2_DROME	Pros25	IntAct
PSA3_DROME	Prosalpha7	IntAct
PSB3_DROME	Prosbeta3	IntAct
Q7KTP7_DROME	CG31919	IntAct
Q8IRD1_DROME	CG12010	IntAct
Q9V436_DROME	Rpn12	BioGRID
Q9VJJ0_DROME	CG17331	IntAct
Q9VJW5_DROME	BG:DS08249.3	IntAct
PRS4_DROME	Pros26.4	IntAct
PSA4_DROME	Pros29	IntAct
PSMD4_DROME	Pros54	IntAct
Q8T8V8_DROME	Prosbeta5R	IntAct
Q9V436_DROME	Rpn12	MINT
Q9V5C6-1	_	IntAct
Q9VJW5_DROME	BG:DS08249.3	BioGRID
Q9VK23_DROME	CG15482	IntAct
Q9VTQ9_DROME	Rpt4R	IntAct
Q9VK23_DROME	CG15482	BioGRID

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

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