SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000280 
UniProt Accession
Theoretical PI
5.3  
Molecular Weight
30088 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
GN ORFNames=CG8979 
Gene Synonyms/Alias
 
Protein Name
Putative proteasome inhibitor 
Protein Synonyms/Alias
 
Organism
Drosophila melanogaster (Fruit fly) 
NCBI Taxonomy ID
7227 
Chromosome Location
chr:2R;7854841-7856348;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. M. Bader, S. Benjamin, O. L. Wapinski, D. M. Smith, A. L. Goldberg and H. Steller (2011) A conserved F box regulatory complex controls proteasome activity in Drosophila. Cell 145(3): 371-82. 

Abstract
The ubiquitin-proteasome system catalyzes the degradation of intracellular proteins. Although ubiquitination of proteins determines their stabilities, there is growing evidence that proteasome function is also regulated. We report the functional characterization of a conserved proteasomal regulatory complex. We identified DmPI31 as a binding partner of the F box protein Nutcracker, a component of an SCF ubiquitin ligase (E3) required for caspase activation during sperm differentiation in Drosophila. DmPI31 binds Nutcracker via a conserved mechanism that is also used by mammalian FBXO7 and PI31. Nutcracker promotes DmPI31 stability, which is necessary for caspase activation, proteasome function, and sperm differentiation. DmPI31 can activate 26S proteasomes in vitro, and increasing DmPI31 levels suppresses defects caused by diminished proteasome activity in vivo. Furthermore, loss of DmPI31 function causes lethality, cell-cycle abnormalities, and defects in protein degradation, demonstrating that DmPI31 is physiologically required for normal proteasome activity. PMID: [21529711] 

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Figures for illustrating the function of this protein/gene
Ref: M. Bader, S. Benjamin, O. L. Wapinski, D. M. Smith, A. L. Goldberg and H. Steller (2011) A conserved F box regulatory complex controls proteasome activity in Drosophila. Cell 145(3): 371-82. PMID: [21529711]
Function
Could play an important role in control of proteasomefunction. Inhibits the hydrolysis of protein and peptidesubstrates by the 20S proteasome (By similarity). 
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Subcellular Location
 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0000502 C:proteasome complex IEA:UniProtKB-KW.
GO:0070864 C:sperm individualization complex IDA:FlyBase.
GO:0008584 P:male gonad development IMP:FlyBase.
GO:0007140 P:male meiosis IMP:FlyBase.
GO:0032436 P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process IMP:FlyBase.
GO:0006974 P:response to DNA damage stimulus IMP:FlyBase.
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Interpro
IPR021625;    Inhibitor_PI31.
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Pfam
PF11566;    PI31_Prot_N;    1.
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SMART
PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    270       Putative proteasome inhibitor.
                             /FTId=PRO_0000220924.
COMPBIAS    169    270       Pro-rich.
MOD_RES     168    168       Phosphoserine.
MOD_RES     189    189       Phosphoserine.
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Nucleotide Sequence
Length: bp   Go to nucleotide: FASTA
Protein Sequence
Length: 270 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
ntcIntAct 
ntcBioGRID 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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