SG ID

SG00000298 

UniProt Accession

Q8T0A7_DROME;Q8T0A7;  

Theoretical PI

5.99  

Molecular Weight

72867 Da  

Genbank Nucleotide ID

AY069440;  

Genbank Protein ID

AAL39585.1; 

Gene Name

aux 

Gene Synonyms/Alias

auxillin 

Protein Name

 

Protein Synonyms/Alias

SubName: LD15107p 

Drosophila melanogaster (Fruit fly) 

7227 

Temporarily unavailable 

The information of related literatures

1. X. Zhou, L. Fabian, J. L. Bayraktar, H. M. Ding, J. A. Brill and H. C. Chang (2011) Auxilin is required for formation of Golgi-derived clathrin-coated vesicles during Drosophila spermatogenesis. Development 138(6): 1111-20. 

Abstract
Clathrin has previously been implicated in Drosophila male fertility and spermatid individualization. To understand further the role of membrane transport in this process, we analyzed the phenotypes of mutations in Drosophila auxilin (aux), a regulator of clathrin function, in spermatogenesis. Like partial loss-of-function Clathrin heavy chain (Chc) mutants, aux mutant males are sterile and produce no mature sperm. The reproductive defects of aux males were rescued by male germ cell-specific expression of aux, indicating that auxilin function is required autonomously in the germ cells. Furthermore, this rescue depends on both the clathrin-binding and J domains, suggesting that the ability of Aux to bind clathrin and the Hsc70 ATPase is essential for sperm formation. aux mutant spermatids show a deficit in formation of the plasma membrane during elongation, which probably disrupts the subsequent coordinated migration of investment cones during individualization. In wild-type germ cells, GFP-tagged clathrin localized to clusters of vesicular structures near the Golgi. These structures also contained the Golgi-associated clathrin adaptor AP-1, suggesting that they were Golgi-derived. By contrast, in aux mutant cells, clathrin localized to abnormal patches surrounding the Golgi and its colocalization with AP-1 was disrupted. Based on these results, we propose that Golgi-derived clathrin-positive vesicles are normally required for sustaining the plasma membrane increase necessary for spermatid differentiation. Our data suggest that Aux participates in forming these Golgi-derived clathrin-positive vesicles and that Aux, therefore, has a role in the secretory pathway. PMID: [21343365] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005795	C:Golgi stack	IDA:FlyBase.
GO:0004672	F:protein kinase activity	NAS:FlyBase.
GO:0001745	P:compound eye morphogenesis	IMP:FlyBase.
GO:0007219	P:Notch signaling pathway	IMP:FlyBase.
GO:0007291	P:sperm individualization	IMP:FlyBase.
GO:0016191	P:synaptic vesicle uncoating	TAS:FlyBase.

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Interpro

IPR008973;    C2_Ca/lipid-bd_dom_CaLB.
IPR001623;    DnaJ_N.
IPR014020;    Tensin_phosphatase_C2-dom.
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Pfam

PF00226;    DnaJ;    1.
PF10409;    PTEN_C2;    1.
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SMART

SM00271;    DnaJ;    1.
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PROSITE

PS51182;    C2_TENSIN;    1.
PS50076;    DNAJ_2;    1.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 2719 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 664 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

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