SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000302 
UniProt Accession
Theoretical PI
6.81  
Molecular Weight
147592 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Gld2 
Gene Synonyms/Alias
ORFNames=CG5732 
Protein Name
Poly(A) RNA polymerase gld-2 homolog A 
Protein Synonyms/Alias
DmGLD2EC=2.7.7.19 
Organism
Drosophila melanogaster (Fruit fly) 
NCBI Taxonomy ID
7227 
Chromosome Location
chr:3R;18037546-18042854;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. C. V. Sartain, J. Cui, R. P. Meisel and M. F. Wolfner (2011) The poly(A) polymerase GLD2 is required for spermatogenesis in Drosophila melanogaster. Development 138(8): 1619-29. 

Abstract
The DNA of a developing sperm is normally inaccessible for transcription for part of spermatogenesis in many animals. In Drosophila melanogaster, many transcripts needed for late spermatid differentiation are synthesized in pre-meiotic spermatocytes, but are not translated until later stages. Thus, post-transcriptional control mechanisms are required to decouple transcription and translation during spermatogenesis. In the female germline, developing germ cells accomplish similar decoupling through poly(A) tail alterations to ensure that dormant transcripts are not prematurely translated PMID: [21427144] 

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Figures for illustrating the function of this protein/gene
Ref: C. V. Sartain, J. Cui, R. P. Meisel and M. F. Wolfner (2011) The poly(A) polymerase GLD2 is required for spermatogenesis in Drosophila melanogaster. Development 138(8): 1619-29. PMID: [21427144]
Ref: C. V. Sartain, J. Cui, R. P. Meisel and M. F. Wolfner (2011) The poly(A) polymerase GLD2 is required for spermatogenesis in Drosophila melanogaster. Development 138(8): 1619-29. PMID: [21427144]
Ref: C. V. Sartain, J. Cui, R. P. Meisel and M. F. Wolfner (2011) The poly(A) polymerase GLD2 is required for spermatogenesis in Drosophila melanogaster. Development 138(8): 1619-29. PMID: [21427144]
Ref: C. V. Sartain, J. Cui, R. P. Meisel and M. F. Wolfner (2011) The poly(A) polymerase GLD2 is required for spermatogenesis in Drosophila melanogaster. Development 138(8): 1619-29. PMID: [21427144]
Function
Cytoplasmic poly(A) RNA polymerase that adds successiveAMP monomers to the 3'-end of specific RNAs, forming a poly(A)tail. In contrast to the canonical nuclear poly(A) RNA polymerase,it only adds poly(A) to selected cytoplasmic mRNAs. Required forformation of long term memory. 
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Subcellular Location
Cytoplasm. Nucleus. Note=Found in neuronalcytoplasm, present in discrete neuronal particles associated withmRNA control, and in nuclear puncta. 
Tissue Specificity
Expressed in the brain. 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm IDA:FlyBase.
GO:0043005 C:neuron projection IDA:FlyBase.
GO:0005654 C:nucleoplasm IDA:FlyBase.
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0046872 F:metal ion binding IEA:UniProtKB-KW.
GO:0004652 F:polynucleotide adenylyltransferase activity ISS:UniProtKB.
GO:0003723 F:RNA binding IEA:UniProtKB-KW.
GO:0007616 P:long-term memory IMP:FlyBase.
GO:0006397 P:mRNA processing IEA:UniProtKB-KW.
GO:0043631 P:RNA polyadenylation ISS:UniProtKB.
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Interpro
IPR002058;    PAP_assoc.
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Pfam
PF03828;    PAP_assoc;    1.
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SMART
PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   1364       Poly(A) RNA polymerase gld-2 homolog A.
                             /FTId=PRO_0000341557.
DOMAIN     1163   1224       PAP-associated.
COMPBIAS      4     11       Poly-Ala.
COMPBIAS     15     20       Poly-Ala.
COMPBIAS    465    491       Ser-rich.
COMPBIAS    599    645       Ala-rich.
METAL       993    993       Magnesium or manganese; catalytic (By
                             similarity).
METAL       995    995       Magnesium or manganese; catalytic (By
                             similarity).
VAR_SEQ     881   1364       PEENNDDDELPLVVHNRYWREFFGYTPADRFLLRAKFVEMR
                             RPPKVMGCKNKWDPLSLSVWKKFLESQQTRHVYKIKMRLWR
                             AIYTVAMKNYPRYGLYLVGSSISYFGSKCSDMDICMLACTN
                             PNIDSRMEAVYHLHVMKELLGRTNMFQDFNLIEARVPILRF
                             TDRCHKVEVDINFNNSVGIRNTHLLYCYSQLDWRVRPMALT
                             VKQWAQYHNINNAKNMTISSYSLMLMVIHFLQVGASPPVLP
                             CLHNLYPEKFGLLQPNDFGYVDMNEVMAPYQSDNSQTLGDL
                             LLSFLHYYSVFDYGKYAISIRVGGVLPIEVCRAATAPKNDI
                             HQWNELCIEEPFDQTNTARSVYDTDTFERIKTIFVASYRRL
                             DSTRNLSAIFEDYDGPTILMQQPSVDSEIELYEGQHHRLLP
                             NRGSSRSNSAIPSPRPSILMVDKATTAIWDDINNKPDHPVL
                             SHSNNYDATNECTGNGSLMGLKDNSVADKPPIA -> VGEL
                             KNVLLNGQITKFISSKSYFFLSSVCLIVHFIRFTK (in
                             isoform B).
                             /FTId=VSP_034331.
MUTAGEN     995    995       D->A: Destroys active site.
CONFLICT    523    523       I -> V (in Ref. 3; AAM52588 and 4;
                             ACH95257).
CONFLICT    591    591       P -> A (in Ref. 3; AAM52588 and 4;
                             ACH95257).
CONFLICT    610    610       T -> TAA (in Ref. 3; AAM52588 and 4;
                             ACH95257).
CONFLICT    636    636       A -> T (in Ref. 3; AAM52588).
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Nucleotide Sequence
Length: 27905053 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 1364 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
eIF4E-5BioGRID 
eIF4E-7IntAct 
eIF4E-5MINT 
eIF4E-7BioGRID 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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