SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000334 
UniProt Accession
Theoretical PI
8.94  
Molecular Weight
73358 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
tef 
Gene Synonyms/Alias
ORFNames=CG8961 
Protein Name
Protein teflon 
Protein Synonyms/Alias
 
Organism
Drosophila melanogaster (Fruit fly) 
NCBI Taxonomy ID
7227 
Chromosome Location
chr:2R;12942048-12944329;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. G. H. Arya, M. J. Lodico, O. I. Ahmad, R. Amin and J. E. Tomkiel (2006) Molecular characterization of teflon, a gene required for meiotic autosome segregation in male Drosophila melanogaster. Genetics 174(1): 125-34. 

Abstract
Drosophila melanogaster males lack recombination and have evolved a mechanism of meiotic chromosome segregation that is independent of both the chiasmatic and achiasmatic segregation systems of females. The teflon (tef) gene is specifically required in males for proper segregation of autosomes and provides a genetic tool for understanding recombination-independent mechanisms of pairing and segregation as well as differences in sex chromosome vs. autosome segregation. Here we report on the cloning of the tef gene and the molecular characterization of tef mutations. Rescue experiments using a GAL4-driven pUAS transgene demonstrate that tef corresponds to predicted Berkeley Drosophila Genome Project (BDGP) gene CG8961 and that tef expression is required in the male germ line prior to spermatocyte stage S4. Consistent with this early prophase requirement, expression of tef was found to be independent of regulators of meiotic M phase initiation or progression. The predicted Tef protein contains three C2H2 zinc-finger motifs, one at the amino terminus and two in tandem at the carboxyl terminus. In addition to the zinc-finger motifs, a 44- to 45-bp repeat is conserved in three related Drosophila species. On the basis of these findings, we propose a role for Tef as a bridging molecule that holds autosome bivalents together via heterochromatic connections. PMID: [16816414] 

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Figures for illustrating the function of this protein/gene
Function
Specifically required in males for proper segregation ofautosomal bivalents at meiosis I. Expression is required in themale germ line prior to spermatocyte stage S4. May have a role asa bridging molecule maintaining adhesion to hold autosomebivalents together via heterochromatic connections. 
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Subcellular Location
Nucleus. Chromosome. Note=Male meioticchromosomes. 
Tissue Specificity
Expressed at a low level in a variety oftissues, highest expression is in testis. 
Gene Ontology
GO IDGO termEvidence
GO:0030849 C:autosome IDA:UniProtKB.
GO:0005634 C:nucleus IEA:UniProtKB-SubCell.
GO:0008270 F:zinc ion binding IEA:InterPro.
GO:0051308 P:male meiosis chromosome separation IMP:UniProtKB.
GO:0007141 P:male meiosis I IMP:FlyBase.
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Interpro
IPR007087;    Znf_C2H2.
IPR015880;    Znf_C2H2-like.
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Pfam
SMART
SM00355;    ZnF_C2H2;    3.
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PROSITE
PS00028;    ZINC_FINGER_C2H2_1;    3.
PS50157;    ZINC_FINGER_C2H2_2;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    649       Protein teflon.
                             /FTId=PRO_0000377408.
ZN_FING      33     56       C2H2-type 1.
ZN_FING     599    621       C2H2-type 2.
ZN_FING     625    648       C2H2-type 3.
VARIANT      90     90       G -> E (in strain: NC732 and NC390).
VARIANT      97     97       D -> Y (in strain: MW6).
VARIANT     122    122       M -> I (in strain: MW11, MW27, NC301 and
                             NC397).
VARIANT     131    131       S -> N (in strain: MW11).
VARIANT     155    155       N -> S (in strain: MW11).
VARIANT     162    162       R -> C (in strain: MW25, MW38, MW56,
                             MW63, MW9, NC304, NC357 and NC361).
VARIANT     211    211       I -> M (in strain: MW6, MW9, MW11, MW25,
                             MW27, MW38, MW56, MW63, NC301, NC304,
                             NC357, NC361, NC390, NC397 and NC732).
VARIANT     240    240       T -> S (in strain: MW11).
VARIANT     292    292       A -> S (in strain: NC357).
VARIANT     346    346       G -> D (in strain: NC397).
VARIANT     349    349       D -> N (in strain: NC335).
VARIANT     383    383       A -> T (in strain: MW6, MW27, NC301,
                             NC304 and NC397).
MUTAGEN      38     38       C->Y: In z5864; Induces 4th chromosome
                             non-disjunction.
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Nucleotide Sequence
Length: 2274 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 649 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
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