SG ID

SG00000340 

UniProt Accession

SPC25_DROME;Q9V3V7;  

Theoretical PI

8.73  

Molecular Weight

25742 Da  

Genbank Nucleotide ID

AF219224; AE014297; AY051881;  

Genbank Protein ID

AAF37563.1; AAF54875.1; AAK93305.1; 

Gene Name

Spc25 

Gene Synonyms/Alias

Cal2, mitch 

Protein Name

Kinetochore protein Spc25 

Protein Synonyms/Alias

Chromosome alignment defect 2; Mitotic chaos; 

Drosophila melanogaster (Fruit fly) 

7227 

Temporarily unavailable 

The information of related literatures

1. B. Williams, G. Leung, H. Maiato, A. Wong, Z. Li, E. V. Williams, C. Kirkpatrick, C. F. Aquadro, C. L. Rieder and M. L. Goldberg (2007) Mitch a rapidly evolving component of the Ndc80 kinetochore complex required for correct chromosome segregation in Drosophila. J Cell Sci 120(Pt 20): 3522-33. 

Abstract
We identified an essential kinetochore protein, Mitch, from a genetic screen in D. melanogaster. Mitch localizes to the kinetochore, and its targeting is independent of microtubules (MTs) and several other known kinetochore components. Animals carrying mutations in mitch die as late third-instar larvae; mitotic neuroblasts in larval brains exhibit high levels of aneuploidy. Analysis of fixed D. melanogaster brains and mitch RNAi in cultured cells, as well as video recordings of cultured mitch mutant neuroblasts, reveal that chromosome alignment in mitch mutants is compromised during spindle formation, with many chromosomes displaying persistent mono-orientation. These misalignments lead to aneuploidy during anaphase. Mutations in mitch also disrupt chromosome behavior during both meiotic divisions in spermatocytes PMID: [17895365] 

Figures for illustrating the function of this protein/gene

Function

Acts as a component of the essential kinetochore-associated Ndc80 complex, which is required for chromosomesegregation and spindle checkpoint activity during meiosis andmitosis. Required for kinetochore integrity and the organizationof stable microtubule binding sites in the outer plate of thekinetochore. Participates in SAC signaling that respondsspecifically to disruptions in spindle microtubule dynamics. 

Subcellular Location

Nucleus. Chromosome, centromere,kinetochore. Note=Independent of microtubules (MTs) and severalother known kinetochore components. Ndc80 complex has polarorientation along the spindle axis. 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0000777	C:condensed chromosome kinetochore	IEA:UniProtKB-SubCell.
GO:0031262	C:Ndc80 complex	IDA:FlyBase.
GO:0005634	C:nucleus	IEA:UniProtKB-SubCell.
GO:0051301	P:cell division	IEA:UniProtKB-KW.
GO:0051311	P:meiotic metaphase plate congression	IMP:UniProtKB.
GO:0000212	P:meiotic spindle organization	IMP:UniProtKB.
GO:0007080	P:mitotic metaphase plate congression	IMP:FlyBase.
GO:0007052	P:mitotic spindle organization	IMP:FlyBase.

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Pfam

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SMART

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PROSITE

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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 953 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 222 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
Q9VML8_DROME	CG8965	BioGRID
Q9VVT5_DROME	dysb	BioGRID
Q9VYB1_DROME	Ndc80	IntAct
RL13A_DROME	RpL13A	BioGRID
Q9VML8_DROME	CG8965	IntAct
Q9VVT5_DROME	dysb	MINT
Q9VYB1_DROME	Ndc80	BioGRID
RL13A_DROME	RpL13A	MINT

Other Protein-Protein interaction resources

String database  

UniProt	Gene Symbol	Ref Databases
ANLN_DROME	scra	String
AURKB_DROME	ial	String
BTHD_DROME	BthD	String
CCNA_DROME	CycA	String
CDK1_DROME	cdc2	String
CID_DROME	cid	String
CKS1_DROME	Cks30A	String
CLASP_DROME	chb	String
DCTN1_DROME	Gl	String
DS_DROME	ds	String
DYHC_DROME	Dhc64C	String
EXO1_DROME	tos	String
GSTT1_DROME	GstD1	String
KL61_DROME	Klp61F	String
LIN1_DROME	CG5198	String
LIS1_DROME	Lis-1	String
MCM10_DROME	Mcm10	String
MCM3_DROME	Mcm3	String
MCM5_DROME	Mcm5	String
MTL14_DROME	CG7818	String
MYC_DROME	dm	String
NANOS_DROME	nos	String
NSE1_DROME	CG11329	String
NXT1_DROME	Nxt1	String
ORC1_DROME	Orc1	String
ORC6_DROME	Orc6	String
OTE_DROME	Ote	String
PARP_DROME	Parp	String
PCNA_DROME	mus209	String
PGAM5_DROME	Pgam5	String
POLO_DROME	polo	String
PORED_DROME	CG7840	String
PRI1_DROME	DNApol-alpha50	String
RIR1_DROME	RnrL	String
RIR2_DROME	RnrS	String
RNH2A_DROME	CG13690	String
SMD1_DROME	SmD1	String
ANLN_DROME	scra	String
AURKB_DROME	ial	String
BTHD_DROME	BthD	String
CCNA_DROME	CycA	String
CDK1_DROME	cdc2	String
CID_DROME	cid	String
CKS1_DROME	Cks30A	String
CLASP_DROME	chb	String
DCTN1_DROME	Gl	String
DS_DROME	ds	String
DYHC_DROME	Dhc64C	String
EXO1_DROME	tos	String
GSTT1_DROME	GstD1	String
KL61_DROME	Klp61F	String
LIN1_DROME	CG5198	String
LIS1_DROME	Lis-1	String
MCM10_DROME	Mcm10	String
MCM3_DROME	Mcm3	String
MCM5_DROME	Mcm5	String
MTL14_DROME	CG7818	String
MYC_DROME	dm	String
NANOS_DROME	nos	String
NSE1_DROME	CG11329	String
NXT1_DROME	Nxt1	String
ORC1_DROME	Orc1	String
ORC6_DROME	Orc6	String
OTE_DROME	Ote	String
PARP_DROME	Parp	String
PCNA_DROME	mus209	String
PGAM5_DROME	Pgam5	String
POLO_DROME	polo	String
PORED_DROME	CG7840	String
PRI1_DROME	DNApol-alpha50	String
RIR1_DROME	RnrL	String
RIR2_DROME	RnrS	String
RNH2A_DROME	CG13690	String
SMD1_DROME	SmD1	String
SPNE_DROME	spn-E	String
THIO1_DROME	dhd	String
THOC5_DROME	thoc5	String
THR_DROME	thr	String
TIPIN_DROME	CG10336	String
TTF2_DROME	lds	String
U488_DROME	CG14286	String
U609_DROME	CG1218	String
WRNXO_DROME	WRNexo	String
ZW10_DROME	mit(1)15	String
ZWILC_DROME	zwilch	String
SPNE_DROME	spn-E	String
THIO1_DROME	dhd	String
THOC5_DROME	thoc5	String
THR_DROME	thr	String
TIPIN_DROME	CG10336	String
TTF2_DROME	lds	String
U488_DROME	CG14286	String
U609_DROME	CG1218	String
WRNXO_DROME	WRNexo	String
ZW10_DROME	mit(1)15	String
ZWILC_DROME	zwilch	String

View Microarray data

Temporarily unavailable 

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