SG ID

SG00000349 

UniProt Accession

Q9VCH1_DROME;Q9VCH1;  

Theoretical PI

8.17  

Molecular Weight

226597 Da  

Genbank Nucleotide ID

AE014297;  

Genbank Protein ID

AAF56194.2; 

Gene Name

eIF4G2 

Gene Synonyms/Alias

ofs 

Protein Name

 

Protein Synonyms/Alias

SubName: Eukaryotic translation initiation factor 4G2 

Drosophila melanogaster (Fruit fly) 

7227 

Temporarily unavailable 

The information of related literatures

1. C. C. Baker and M. T. Fuller (2007) Translational control of meiotic cell cycle progression and spermatid differentiation in male germ cells by a novel eIF4G homolog. Development 134(15): 2863-9. 

Abstract
Translational control is crucial for proper timing of developmental events that take place in the absence of transcription, as in meiotic activation in oocytes, early embryogenesis in many organisms, and spermatogenesis. Here we show that a novel form of the translation initiation complex component eIF4G in Drosophila, eIF4G2, is required specifically for male germ cells to undergo meiotic division and proper spermatid differentiation. Flies mutant for eIF4G2 are viable and female fertile but male sterile. Spermatocytes form, but the germ cells in mutant males skip the major events of the meiotic divisions and form aberrant spermatids with large nuclei. Consistent with the failure to undergo the meiotic divisions, function of eIF4G2 is required post-transcriptionally for normal accumulation of the core cell cycle regulatory proteins Twine and CycB in mature spermatocytes. Loss of eIF4G2 function also causes widespread defects in spermatid differentiation. Although differentiation markers Dj and Fzo are expressed in late-stage eIF4G2 mutant germ cells, several key steps of spermatid differentiation fail, including formation of a compact mitochondrial derivative and full elongation. Our results suggest that an alternate form of the translation initiation machinery may be required for regulation and execution of key steps in male germ cell differentiation. PMID: [17611220] 

2. T. M. Franklin-Dumont, C. Chatterjee, S. A. Wasserman and S. Dinardo (2007) A novel eIF4G homolog, Off-schedule, couples translational control to meiosis and differentiation in Drosophila spermatocytes. Development 134(15): 2851-61. 

Abstract
During spermatogenesis, cells coordinate differentiation with the meiotic cell cycle to generate functional gametes. We identified a novel gene, which we named off-schedule (ofs), as being essential for this coordinated control. During the meiotic G(2) phase, Drosophila ofs mutant germ cells do not reach their proper size and fail to execute meiosis or significant differentiation. The accumulation of four cell cycle regulators--Cyclin A, Boule, Twine and Roughex--is altered in these mutants, indicating that ofs reveals a novel branch of the pathway controlling meiosis and differentiation. Ofs is homologous to eukaryotic translation initiation factor eIF4G. The level of ofs expression in spermatocytes is much higher than for the known eIF4G ortholog (known as eIF-4G or eIF4G), suggesting that Ofs substitutes for this protein. Consistent with this, assays for association with mRNA cap complexes, as well as RNA-interference and phenotypic-rescue experiments, demonstrate that Ofs has eIF4G activity. Based on these studies, we speculate that spermatocytes monitor G(2) growth as one means to coordinate the initiation of meiotic division and differentiation. PMID: [17611222] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0003677	F:DNA binding	IEA:InterPro.
GO:0000340	F:RNA 7-methylguanosine cap binding	IDA:FlyBase.
GO:0003743	F:translation initiation factor activity	IEA:UniProtKB-KW.
GO:0000082	P:G1/S transition of mitotic cell cycle	IGI:FlyBase.
GO:0007140	P:male meiosis	IMP:FlyBase.
GO:0008315	P:meiotic G2/MI transition	IMP:FlyBase.
GO:0016070	P:RNA metabolic process	IEA:InterPro.
GO:0035071	P:salivary gland cell autophagic cell death	IEP:FlyBase.
GO:0048515	P:spermatid differentiation	IMP:FlyBase.

Back to Top

Interpro

IPR016024;    ARM-type_fold.
IPR003891;    Initiation_fac_eIF4g_MI.
IPR016021;    MIF4-like_typ_1/2/3.
IPR003890;    MIF4G-like_typ-3.
Back to Top

Pfam

PF02847;    MA3;    1.
PF02854;    MIF4G;    1.
Back to Top

SMART

SM00544;    MA3;    1.
SM00543;    MIF4G;    1.
Back to Top

PROSITE

PS51366;    MI;    1.
Back to Top

PRINTS

Back to Top

Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 27905053 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 2072 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
A1Z8Z8_DROME	CG13157	IntAct
A1ZBV0_DROME	CG13427	IntAct
ATTA_DROME	AttA	IntAct
CP4G1_DROME	Cyp4g1	IntAct
G6PI_DROME	Pgi	IntAct
O16011-3	mbl	IntAct
O77259_DROME	mod(r)	IntAct
P27619-2	shi	IntAct
Q7K485_DROME	cathD	IntAct
Q8IQV7_DROME	CG6933-RA	IntAct
Q8T3W6_DROME	hale	IntAct
Q95RS6_DROME	CG40160	IntAct
Q95T37_DROME	Nmda1	IntAct
Q9V3U6_DROME	26-29-p	IntAct
Q9VBI1_DROME	Sld5	MINT
A1Z8Z8_DROME	CG13157	BioGRID
A1ZBV0_DROME	CG13427	BioGRID
A4V4I8_DROME	shi	BioGRID
A4V4J0_DROME	shi	BioGRID
A8DYS5_DROME	Cda5	BioGRID
ATTA_DROME	AttA	MINT
B7YZI9_DROME	mbl	BioGRID
B7YZJ0_DROME	mbl	BioGRID
C9QPI9_DROME	CycB-RB	BioGRID
CCNB_DROME	CycB	BioGRID
CP4G1_DROME	Cyp4g1	BioGRID
DYN_DROME	shi	BioGRID
E1JH88_DROME	mbl	BioGRID
E1JJA4_DROME	shi	BioGRID
E1JJA5_DROME	shi	BioGRID
E2QCY2_DROME	CG3961	BioGRID
G6PI_DROME	Pgi	BioGRID
MBL_DROME	mbl	BioGRID
O16011-3	mbl	MINT
O77259_DROME	mod(r)	BioGRID
Q0E8E2_DROME	CG4998	BioGRID
Q0E8V4_DROME	Cda5	BioGRID
Q7JR91_DROME	Nmda1	BioGRID
Q7JRM9_DROME	Nmda1	BioGRID
Q7K485_DROME	cathD	BioGRID
Q7KWG9_DROME	CG17018-RD	BioGRID
Q7KWH0_DROME	CG17018-RC	BioGRID
Q7YZA5_DROME	mod(r)	BioGRID
Q8IQV7_DROME	CG6933-RA	BioGRID
Q8IR80_DROME	Rab40	BioGRID
Q8MR04_DROME	CG6933	BioGRID
Q8T3W6_DROME	hale	BioGRID
Q95RS6_DROME	CG40160	BioGRID
Q95T37_DROME	Nmda1	BioGRID
Q9V313	_	MINT
Q9V3U6_DROME	26-29-p	BioGRID
Q9V6B4	_	MINT
Q9V936	_	MINT
Q9VBI1_DROME	Sld5	BioGRID
Q9VD17_DROME	CG13857	BioGRID
Q9VNX3_DROME	CG14563	BioGRID
Q9VPI3_DROME	Cda5	BioGRID
Q9VPI4_DROME	Cda5	BioGRID
Q9VS81_DROME	CG7548	BioGRID
Q9VV11_DROME	CG13067	BioGRID
Q9VV38_DROME	CG4998	BioGRID
Q9VV44_DROME	Cpr72Ea	BioGRID
Q9VVT8_DROME	CG3961-RA	BioGRID
Q9VW94_DROME	CG6933-RC	BioGRID
Q9VYM8_DROME	Rab40	BioGRID
Q9VZI1_DROME	Chd64	BioGRID
Q9W106_DROME	CG3589	BioGRID
Q9W140_DROME	CG3548	BioGRID
Q9W219_DROME	CG13512	BioGRID
Q9W5Y4_DROME	CG17018-RC	BioGRID
ROGDI_DROME	rogdi	BioGRID
RS25_DROME	RpS25	BioGRID
Q9VD17_DROME	CG13857	IntAct
Q9VNX3_DROME	CG14563	IntAct
Q9VPI4_DROME	Cda5	IntAct
Q9VS81_DROME	CG7548	IntAct
Q9VV11_DROME	CG13067	IntAct
Q9VV38_DROME	CG4998	IntAct
Q9VV44_DROME	Cpr72Ea	IntAct
Q9VVT8_DROME	CG3961-RA	IntAct
Q9VYM8_DROME	Rab40	IntAct
Q9VZI1_DROME	Chd64	IntAct
Q9W106_DROME	CG3589	IntAct
Q9W140_DROME	CG3548	IntAct
Q9W219_DROME	CG13512	IntAct
Q9W5Y4_DROME	CG17018-RC	IntAct
ROGDI_DROME	rogdi	IntAct

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

Comments