SG ID

SG00000355 

UniProt Accession

Q8IP45_DROME;Q8IP45;  

Theoretical PI

9.15  

Molecular Weight

106858 Da  

Genbank Nucleotide ID

AE014134; BT133182;  

Genbank Protein ID

AAN10896.2; AFA28423.1; 

Gene Name

Cul-3 

Gene Synonyms/Alias

cul-3, Cul-3-RF 

Protein Name

 

Protein Synonyms/Alias

SubName: Cullin 3, isoform FEC=6.3.2.19SubName: FI19425p1 

Drosophila melanogaster (Fruit fly) 

7227 

Temporarily unavailable 

The information of related literatures

1. E. Arama, M. Bader, G. E. Rieckhof and H. Steller (2007) A ubiquitin ligase complex regulates caspase activation during sperm differentiation in Drosophila. PLoS Biol 5(10): e251. 

Abstract
In both insects and mammals, spermatids eliminate their bulk cytoplasm as they undergo terminal differentiation. In Drosophila, this process of dramatic cellular remodeling requires apoptotic proteins, including caspases. To gain further insight into the regulation of caspases, we screened a large collection of sterile male flies for mutants that block effector caspase activation at the onset of spermatid individualization. Here, we describe the identification and characterization of a testis-specific, Cullin-3-dependent ubiquitin ligase complex that is required for caspase activation in spermatids. Mutations in either a testis-specific isoform of Cullin-3 (Cul3(Testis)), the small RING protein Roc1b, or a Drosophila orthologue of the mammalian BTB-Kelch protein Klhl10 all reduce or eliminate effector caspase activation in spermatids. Importantly, all three genes encode proteins that can physically interact to form a ubiquitin ligase complex. Roc1b binds to the catalytic core of Cullin-3, and Klhl10 binds specifically to a unique testis-specific N-terminal Cullin-3 (TeNC) domain of Cul3(Testis) that is required for activation of effector caspase in spermatids. Finally, the BIR domain region of the giant inhibitor of apoptosis-like protein dBruce is sufficient to bind to Klhl10, which is consistent with the idea that dBruce is a substrate for the Cullin-3-based E3-ligase complex. These findings reveal a novel role of Cullin-based ubiquitin ligases in caspase regulation. PMID: [17880263] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0031463	C:Cul3-RING ubiquitin ligase complex	IPI:FlyBase.
GO:0019005	C:SCF ubiquitin ligase complex	NAS:FlyBase.
GO:0004842	F:ubiquitin-protein ligase activity	NAS:FlyBase.
GO:0006919	P:activation of cysteine-type endopeptidase activity involved in apoptotic process	IMP:FlyBase.
GO:0007409	P:axonogenesis	IMP:FlyBase.
GO:0001745	P:compound eye morphogenesis	NAS:FlyBase.
GO:0048813	P:dendrite morphogenesis	IMP:FlyBase.
GO:0007476	P:imaginal disc-derived wing morphogenesis	IMP:FlyBase.
GO:0030162	P:regulation of proteolysis	NAS:FlyBase.
GO:0030431	P:sleep	IMP:FlyBase.
GO:0007291	P:sperm individualization	IMP:FlyBase.
GO:0006511	P:ubiquitin-dependent protein catabolic process	IEA:InterPro.

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Interpro

IPR016157;    Cullin_CS.
IPR016158;    Cullin_homology.
IPR001373;    Cullin_N.
IPR019559;    Cullin_neddylation_domain.
IPR016159;    Cullin_repeat-like_dom.
IPR011991;    WHTH_trsnscrt_rep_DNA-bd.
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Pfam

PF00888;    Cullin;    1.
PF10557;    Cullin_Nedd8;    1.
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SMART

SM00182;    CULLIN;    1.
SM00884;    Cullin_Nedd8;    1.
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PROSITE

PS01256;    CULLIN_1;    1.
PS50069;    CULLIN_2;    1.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 934 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
B7Z114_DROME	Roc1a	BioGRID
A2VEF7_DROME	Dll	BioGRID
A4V3J9_DROME	Doa	BioGRID
A8JRF7_DROME	Doa	BioGRID
B7Z0R6_DROME	Doa	BioGRID
C6SV45_DROME	spn-A-RA	BioGRID
CRN_DROME	crn	BioGRID
DFD_DROME	Dfd	BioGRID
DLL_DROME	Dll	BioGRID
DOA_DROME	Doa	BioGRID
E1JJ11_DROME	Doa	BioGRID
E2QCG7_DROME	eIF3-S9	BioGRID
EIF3B_DROME	eIF3-S9	BioGRID
Q0KI03_DROME	Doa	BioGRID
Q86B72_DROME	Doa	BioGRID
Q86B73_DROME	Doa	BioGRID
Q8IML9_DROME	Doa	BioGRID
Q9VBZ6_DROME	Smg6	BioGRID
Q9VHV1_DROME	DNApol-iota	BioGRID
Q9VL27_DROME	CG5731	BioGRID
Q9VX47_DROME	CG12432	BioGRID
Q9VZM7_DROME	CG1120	BioGRID
RAD51_DROME	spn-A	BioGRID
RBX1A_DROME	Roc1a	BioGRID
RBX1B_DROME	Roc1b	BioGRID

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

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