SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000363 
UniProt Accession
Theoretical PI
6.34  
Molecular Weight
145958 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
bru 
Gene Synonyms/Alias
ORFNames=CG2478 
Protein Name
Protein brunelleschi 
Protein Synonyms/Alias
NIK- and IKBKB-binding protein; 
Organism
Drosophila melanogaster (Fruit fly) 
NCBI Taxonomy ID
7227 
Chromosome Location
chr:2L;20650755-20656176;-1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. C. C. Robinett, M. G. Giansanti, M. Gatti and M. T. Fuller (2009) TRAPPII is required for cleavage furrow ingression and localization of Rab11 in dividing male meiotic cells of Drosophila. J Cell Sci 122(Pt 24): 4526-34. 

Abstract
Although membrane addition is crucial for cytokinesis in many animal cell types, the specific mechanisms supporting cleavage furrow ingression are not yet understood. Mutations in the gene brunelleschi (bru), which encodes the Drosophila ortholog of the yeast Trs120p subunit of TRAPPII, cause failure of furrow ingression in male meiotic cells. In non-dividing cells, Brunelleschi protein fused to GFP is dispersed throughout the cytoplasm and enriched at Golgi organelles, similarly to another Drosophila TRAPPII subunit, dBet3. Localization of the membrane-trafficking GTPase Rab11 to the cleavage furrow requires wild-type function of bru, and genetic interactions between bru and Rab11 increase the failure of meiotic cytokinesis and cause synthetic lethality. bru also genetically interacts with four wheel drive (fwd), which encodes a PI4Kbeta, such that double mutants exhibit enhanced failure of male meiotic cytokinesis. These results suggest that Bru cooperates with Rab11 and PI4Kbeta to regulate the efficiency of membrane addition to the cleavage furrow, thus promoting cytokinesis in Drosophila male meiotic cells. PMID: [19934220] 

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Figures for illustrating the function of this protein/gene
Ref: C. C. Robinett, M. G. Giansanti, M. Gatti and M. T. Fuller (2009) TRAPPII is required for cleavage furrow ingression and localization of Rab11 in dividing male meiotic cells of Drosophila. J Cell Sci 122(Pt 24): 4526-34. PMID: [19934220]
Ref: C. C. Robinett, M. G. Giansanti, M. Gatti and M. T. Fuller (2009) TRAPPII is required for cleavage furrow ingression and localization of Rab11 in dividing male meiotic cells of Drosophila. J Cell Sci 122(Pt 24): 4526-34. PMID: [19934220]
Ref: C. C. Robinett, M. G. Giansanti, M. Gatti and M. T. Fuller (2009) TRAPPII is required for cleavage furrow ingression and localization of Rab11 in dividing male meiotic cells of Drosophila. J Cell Sci 122(Pt 24): 4526-34. PMID: [19934220]
Ref: C. C. Robinett, M. G. Giansanti, M. Gatti and M. T. Fuller (2009) TRAPPII is required for cleavage furrow ingression and localization of Rab11 in dividing male meiotic cells of Drosophila. J Cell Sci 122(Pt 24): 4526-34. PMID: [19934220]
Ref: C. C. Robinett, M. G. Giansanti, M. Gatti and M. T. Fuller (2009) TRAPPII is required for cleavage furrow ingression and localization of Rab11 in dividing male meiotic cells of Drosophila. J Cell Sci 122(Pt 24): 4526-34. PMID: [19934220]
Ref: C. C. Robinett, M. G. Giansanti, M. Gatti and M. T. Fuller (2009) TRAPPII is required for cleavage furrow ingression and localization of Rab11 in dividing male meiotic cells of Drosophila. J Cell Sci 122(Pt 24): 4526-34. PMID: [19934220]
Function
Cooperates with Rab11 and fwd/PI4K to mediate the flowof membrane through the Golgi, which is required to supportcleavage furrow ingression, therefore promoting cytokinesis inmale meiotic cells. 
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Subcellular Location
Cytoplasm. Golgi apparatus. Note=Specific tospermatocytes. 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0036063 C:acroblast IDA:FlyBase.
GO:0000916 P:actomyosin contractile ring contraction IMP:FlyBase.
GO:0007110 P:cytokinesis after meiosis I IMP:FlyBase.
GO:0007111 P:cytokinesis after meiosis II IMP:FlyBase.
GO:0007112 P:male meiosis cytokinesis IMP:FlyBase.
GO:0043147 P:meiotic spindle stabilization IMP:FlyBase.
GO:0048137 P:spermatocyte division IMP:FlyBase.
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Interpro
IPR013935;    TRAPP_II_complex_Trs120.
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Pfam
PF08626;    TRAPPC9-Trs120;    1.
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SMART
PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   1320       Protein brunelleschi.
                             /FTId=PRO_0000372863.
COMPBIAS    363    398       Ser-rich.
COMPBIAS    969    975       Poly-Gly.
MOD_RES     317    317       Phosphoserine.
MOD_RES     329    329       Phosphothreonine.
MOD_RES     672    672       Phosphoserine.
CONFLICT     83     83       H -> Y (in Ref. 3; ABA81812).
CONFLICT    204    204       V -> I (in Ref. 3; ABA81812).
CONFLICT    220    220       V -> M (in Ref. 3; ABA81812).
CONFLICT    354    354       S -> C (in Ref. 3; ABA81812).
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Nucleotide Sequence
Length: bp   Go to nucleotide: FASTA
Protein Sequence
Length: 1320 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Arf51FBioGRID 
Arf51FIntAct 
Snr1IntAct 
Snr1BioGRID 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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