SG ID

SG00000369 

UniProt Accession

Q9VDY8_DROME;Q9VDY8;  

Theoretical PI

5.73  

Molecular Weight

31287 Da  

Genbank Nucleotide ID

AE014297; AF246993; BT083455;  

Genbank Protein ID

AAF55650.1; AAF61273.1; ACR16680.1; 

Gene Name

vib 

Gene Synonyms/Alias

PITP, vib-RA 

Protein Name

 

Protein Synonyms/Alias

SubName: MIP08680pSubName: Phosphatidylinositol transfer proteinSubName: Vibrator 

Drosophila melanogaster (Fruit fly) 

7227 

Temporarily unavailable 

The information of related literatures

1. M. K. Gatt and D. M. Glover (2006) The Drosophila phosphatidylinositol transfer protein encoded by vibrator is essential to maintain cleavage-furrow ingression in cytokinesis. J Cell Sci 119(Pt 11): 2225-35. 

Abstract
Cytokinesis requires the coordination of cytoskeletal and plasma membrane dynamics. A role for phosphatidylinositol lipids has been proposed for the successful completion of cytokinesis but this is still poorly characterised. Here, we show mutants of the gene vibrator, previously found to encode the Drosophila phosphatidylinositol transfer protein, produce multinucleate cells indicative of cytokinesis failure in male meiosis. Examination of fixed preparations of mutant spermatocytes showed contractile rings of anillin and actin that were of normal appearance at early stages but were larger and less well organised at later stages of cytokinesis than in wild-type cells. Time-lapse imaging revealed sequential defects in cytokinesis of vibrator spermatocytes. In cells that fail cytokinesis, central spindle formation occurred correctly, but furrow ingression was delayed and the central spindle did not become compressed to the extent seen in wild-type cells. Cells then stalled at this point before the apparent connection between the constricted cytoskeleton and the plasma membrane was lost; the furrow then underwent elastic regression. We discuss these defects in relation to multiple functions of phosphoinositol lipids in regulating actin dynamics and membrane synthesis. PMID: [16684816] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0032154	C:cleavage furrow	IDA:FlyBase.
GO:0016006	C:Nebenkern	IDA:FlyBase.
GO:0031965	C:nuclear membrane	IDA:FlyBase.
GO:0005886	C:plasma membrane	IDA:FlyBase.
GO:0070732	C:spindle envelope	IDA:FlyBase.
GO:0000916	P:actomyosin contractile ring contraction	IMP:FlyBase.
GO:0031566	P:actomyosin contractile ring maintenance	IMP:FlyBase.
GO:0007110	P:cytokinesis after meiosis I	IMP:FlyBase.
GO:0007111	P:cytokinesis after meiosis II	IMP:FlyBase.
GO:0000915	P:cytokinesis, actomyosin contractile ring assembly	IMP:FlyBase.
GO:0001700	P:embryonic development via the syncytial blastoderm	IMP:FlyBase.
GO:0007112	P:male meiosis cytokinesis	IMP:FlyBase.
GO:0043147	P:meiotic spindle stabilization	IMP:FlyBase.
GO:0048137	P:spermatocyte division	IMP:FlyBase.
GO:0006810	P:transport	IEA:InterPro.

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Interpro

IPR001666;    PI_transfer.
IPR023393;    START-like_dom.
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Pfam

PF02121;    IP_trans;    1.
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SMART

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PROSITE

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PRINTS

PR00391;    PITRANSFER.;   
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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 27905053 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 272 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

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Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

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View Microarray data

Temporarily unavailable 

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