SG ID

SG00000384 

UniProt Accession

F7DUE3_HORSE;F7DUE3;  

Theoretical PI

FRAGMENT  

Molecular Weight

11059 Da  

Genbank Nucleotide ID

 

Genbank Protein ID

 

Gene Name

INSL3 

Gene Synonyms/Alias

 

Protein Name

 

Protein Synonyms/Alias

SubName: Uncharacterized proteinFlags: Fragment 

Equus caballus (Horse) 

9796 

Temporarily unavailable 

The information of related literatures

1. T. Klonisch, K. Steger, A. Kehlen, W. R. Allen, C. Froehlich, J. Kauffold, M. Bergmann and S. Hombach-Klonisch (2003) INSL3 ligand-receptor system in the equine testis. Biol Reprod 68(6): 1975-81. 

Abstract
We employed molecular and immunological techniques to investigate the expression of INSL3, a member of the insulin-like superfamily, in prepubertal testis, postpubertal testes exhibiting normal and disturbed spermatogenesis, and cryptorchid testes of male horses. In addition, the partial cDNA coding sequences of the equine homologue of the human relaxin/INSL3-receptor Lgr8 were determined. Nonradioactive in-situ hybridization with a cRNA probe for equine Insl3 and immunohistochemistry with a specific rabbit INSL3 antiserum localized Insl3 transcripts and immunoreactive INSL3 ligand to Leydig cells in all types of testes investigated. Quantitative polymerase chain reaction analysis revealed a down-regulation of Insl3 and an up-regulation of the relaxin/INSL3-receptor expression in unilateral cryptorchid versus descended testes. Western blot analysis of protein extracts from adult normal and cryptorchid testes and prepubertal testes showed a single immunoreactive band at 14.5 kDa, which correlates with the predicted size of equine proINSL3. Densitometric analysis of Western blot data of adult normal testes revealed significantly stronger expression of immunoreactive proINSL3 as compared to extracts derived from cryptorchid or prepubertal testes. Thus, decreased expression of immunoreactive INSL3 in cryptorchid and prepubertal equine testis is transcriptionally regulated. The detection of transcripts for equine Lgr8 in the testis has identified the testis as a potential target of INSL3. PMID: [12606415] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

Secreted (By similarity). 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005576	C:extracellular region	IEA:UniProtKB-SubCell.
GO:0001701	P:in utero embryonic development	IEA:Compara.
GO:0008584	P:male gonad development	IEA:Compara.

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Interpro

IPR016179;    Insulin-like.
IPR022353;    Insulin_CS.
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Pfam

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SMART

SM00078;    IlGF;    1.
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PROSITE

PS00262;    INSULIN;    1.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 99 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

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Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

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View Microarray data

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