SG ID

SG00000387 

UniProt Accession

F6YQR2_HORSE;F6YQR2;  

Theoretical PI

FRAGMENT  

Molecular Weight

113874 Da  

Genbank Nucleotide ID

 

Genbank Protein ID

 

Gene Name

STK31 

Gene Synonyms/Alias

 

Protein Name

 

Protein Synonyms/Alias

SubName: Uncharacterized proteinFlags: Fragment 

Equus caballus (Horse) 

9796 

Temporarily unavailable 

The information of related literatures

1. K. Sabeur, B. A. Ball, C. J. Corbin and A. Conley (2008) Characterization of a novel, testis-specific equine serine/threonine kinase. Mol Reprod Dev 75(5): 867-73. 

Abstract
Testis-specific protein kinases are important because of their potential role in spermiogenesis, sperm maturation, and sperm function. In the present study, a novel serine-threonine kinase with high identity to human serine-threonine kinase 31 (STK31) was cloned from equine testis and expression of the protein was characterized in equine testis and ejaculated spermatozoa. Five over-lapping independent clones were plaque purified after screening of a lambda ZAP cDNA expression library constructed from equine testis. Sequence analysis and alignment of all five clones showed high identity with human STK31 with approximately 200 bp of the equine N-terminal sequence incomplete. The putative full-length coding sequence of this testis specific equine cDNA was completed by amplification of a 200-bp fragment using a human primer upstream of the reported translational start site with equine specific nested primers. Northern blot analysis using the equine STK31 cDNA detected an RNA transcript of approximately 3.1 kb present in testis but not in other reproductive or somatic tissues. Immunolocalization of the protein in equine testis and spermatozoa demonstrated that STK31 was present in post-meiotic germ cells with localization to the equatorial segment of testicular spermatozoa. Analysis of the domain structure of equine STK31 revealed a protein kinase domain along with a putative RNA-binding region. The post-meiotic expression of this protein along with its domain structure suggests that STK31 may have a role in reorganization of sperm chromatin during spermiogenesis. The cloning of this novel, testis-specific equine STK provides a new tool to explore the role of kinases in sperm function. PMID: [18246530] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005524	F:ATP binding	IEA:InterPro.
GO:0016788	F:hydrolase activity, acting on ester bonds	IEA:InterPro.
GO:0003676	F:nucleic acid binding	IEA:InterPro.
GO:0004672	F:protein kinase activity	IEA:InterPro.

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Interpro

IPR011009;    Kinase-like_dom.
IPR000719;    Prot_kinase_cat_dom.
IPR006021;    Staphylococcal_nuclease.
IPR002999;    Tudor.
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Pfam

PF00069;    Pkinase;    1.
PF00567;    TUDOR;    1.
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SMART

SM00333;    TUDOR;    1.
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PROSITE

PS50011;    PROTEIN_KINASE_DOM;    1.
PS50304;    TUDOR;    1.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 1003 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

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