SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000404 
UniProt Accession
Theoretical PI
5.22  
Molecular Weight
104553 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
AP2B1 
Gene Synonyms/Alias
ADTB2, CLAPB1 
Protein Name
AP-2 complex subunit beta 
Protein Synonyms/Alias
AP105B; Adapter-related protein complex 2 beta subunit; Adaptor protein complex AP-2 subunit beta; Beta-2-adaptin; Beta-adaptin; Clathrin assembly protein complex 2 beta large chain; Plasma membrane adaptor HA2/AP2 adaptin beta subunit; 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:17;33914282-34053434;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Ap2beta-NY was variably expressed in the testes of patients with spermatogenesis-disturbance and spermatogenesis-arrest ; in the testis, Ap2beta-NY was restrictively expressed in germ cells. 
The information of related literatures
1. X. D. Zhang, L. L. Yin, Y. Zheng, L. Lu, Z. M. Zhou and J. H. Sha (2005) Expression of a novel beta adaptin subunit mRNA splice variant in human testes. Asian J Androl 7(2): 179-88. 

Abstract
AIM PMID: [15897975] 

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Figures for illustrating the function of this protein/gene
Function
Component of the adaptor protein complex 2 (AP-2).Adaptor protein complexes function in protein transport viatransport vesicles in different membrane traffic pathways. Adaptorprotein complexes are vesicle coat components and appear to beinvolved in cargo selection and vesicle formation. AP-2 isinvolved in clathrin-dependent endocytosis in which cargo proteinsare incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with theearly endosome. The clathrin lattice serves as a mechanicalscaffold but is itself unable to bind directly to membranecomponents. Clathrin-associated adaptor protein (AP) complexeswhich can bind directly to both the clathrin lattice and to thelipid and protein components of membranes are considered to be themajor clathrin adaptors contributing the CCV formation. AP-2 alsoserves as a cargo receptor to selectively sort the membraneproteins involved in receptor-mediated endocytosis. AP-2 seems toplay a role in the recycling of synaptic vesicle membranes fromthe presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi)and [ED]-X-X-X-L-[LI] endocytosis signal motifs within thecytosolic tails of transmembrane cargo molecules. AP-2 may alsoplay a role in maintaining normal post-endocytic traffickingthrough the ARF6-regulated, non-clathrin pathway. The AP-2 betasubunit acts via its C-terminal appendage domain as a scaffoldingplatform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds toclathrin heavy chain, promoting clathrin lattice assembly;clathrin displaces at least some CLASPs from AP2B1 which probablythen can be positioned for further coat assembly. 
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Subcellular Location
Cell membrane. Membrane, coated pit;Peripheral membrane protein; Cytoplasmic side. Note=AP-2 appearsto be excluded from internalizing CCVs and to disengage from sitesof endocytosis seconds before internalization of the nascent CCV. 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0030131 C:clathrin adaptor complex IEA:InterPro.
GO:0005905 C:coated pit IEA:UniProtKB-SubCell.
GO:0005829 C:cytosol TAS:Reactome.
GO:0030666 C:endocytic vesicle membrane TAS:Reactome.
GO:0005886 C:plasma membrane TAS:Reactome.
GO:0005802 C:trans-Golgi network IEA:Compara.
GO:0008565 F:protein transporter activity IEA:InterPro.
GO:0007411 P:axon guidance TAS:Reactome.
GO:0048268 P:clathrin coat assembly IEA:Compara.
GO:0007173 P:epidermal growth factor receptor signaling pathway TAS:Reactome.
GO:0006886 P:intracellular protein transport IEA:InterPro.
GO:0042059 P:negative regulation of epidermal growth factor receptor signaling pathway TAS:Reactome.
GO:0048011 P:nerve growth factor receptor signaling pathway TAS:Reactome.
GO:0007269 P:neurotransmitter secretion IEA:Compara.
GO:0050690 P:regulation of defense response to virus by virus TAS:Reactome.
GO:0007268 P:synaptic transmission TAS:Reactome.
GO:0048488 P:synaptic vesicle endocytosis IEA:Compara.
GO:0016032 P:viral reproduction TAS:Reactome.
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Interpro
IPR016342;    AP_complex_bsu.
IPR011989;    ARM-like.
IPR016024;    ARM-type_fold.
IPR015151;    B-adaptin_app_sub_C.
IPR012295;    Beta2_adaptin/TBP_C_dom.
IPR002553;    Clathrin/coatomer_adapt-like_N.
IPR008152;    Clathrin_a/b/g-adaptin_app_Ig.
IPR013037;    Clathrin_b-adaptin_app_Ig-like.
IPR009028;    Coatomer/calthrin_app_sub_C.
IPR013041;    Coatomer/clathrin_app_Ig-like.
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Pfam
PF01602;    Adaptin_N;    1.
PF02883;    Alpha_adaptinC2;    1.
PF09066;    B2-adapt-app_C;    1.
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SMART
SM00809;    Alpha_adaptinC2;    1.
SM01020;    B2-adapt-app_C;    1.
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PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    937       AP-2 complex subunit beta.
                             /FTId=PRO_0000193742.
REGION      841    937       Interaction with ARRB1.
COMPBIAS    576    716       Pro-rich (stalk region).
MOD_RES       2      2       Phosphothreonine.
MOD_RES       4      4       Phosphoserine.
MOD_RES     265    265       N6-acetyllysine.
MOD_RES     276    276       Phosphotyrosine.
MOD_RES     318    318       N6-acetyllysine.
MOD_RES     737    737       Phosphotyrosine; by SRC.
MOD_RES     888    888       Phosphotyrosine.
VAR_SEQ     663    663       L -> LLGSDLGGGIGGSPA (in isoform 2).
                             /FTId=VSP_011490.
MUTAGEN     815    815       Y->A: Strongly reduces interaction with
                             SNAP91, EPS15, AMPH and BIN1 and clathrin
                             heavy chain.
MUTAGEN     841    841       W->A: Abolishes interaction with LDLRAP1
                             and ARRB1. Greatly reduces DENND1B-
                             binding.
MUTAGEN     842    842       K->E: Strongly reduces interaction with
                             ARRB1.
MUTAGEN     849    849       E->A: Strongly reduces interaction with
                             LDLRAP1, ARRB1 and EPN1. No effect on
                             DENND1B-binding.
MUTAGEN     851    851       Q->A: Strongly reduces interaction with
                             ARRB1.
MUTAGEN     879    879       R->A: No effect on interaction with
                             ARRB1.
MUTAGEN     879    879       R->E: Strongly reduces interaction with
                             EPN1. Reduces interaction with SNAP91 and
                             clathrin. No effect on EPS15 binding.
MUTAGEN     888    888       Y->V: Strongly reduces interaction with
                             SNAP91, EPN1 and clathrin. No effect on
                             EPS15 binding. Abolishes interaction with
                             ARRB1 and with DENND1B.
MUTAGEN     902    902       E->A: Strongly reduces interaction with
                             LDLRAP1 and ARRB1. No effect on DENND1B-
                             binding.
MUTAGEN     917    917       K->Q: Strongly reduces interaction with
                             LDLRAP1. SNAP91 and clathrin. Reduces
                             interaction with EPN1. No effect on EPS15
                             binding.
STRAND       11     13
HELIX        14     21
HELIX        27     42
HELIX        48     50
HELIX        51     56
STRAND       59     61
HELIX        63     79
HELIX        81     85
HELIX        88     91
HELIX        92     94
STRAND       95     99
HELIX       100    111
HELIX       116    118
HELIX       119    129
HELIX       135    150
HELIX       156    167
HELIX       174    177
HELIX       180    187
TURN        192    195
HELIX       200    213
HELIX       216    227
HELIX       234    244
HELIX       245    247
HELIX       254    265
STRAND      267    270
TURN        272    275
HELIX       277    283
HELIX       285    291
HELIX       296    312
TURN        316    319
TURN        321    324
HELIX       332    344
TURN        348    350
HELIX       351    361
HELIX       367    381
HELIX       385    400
HELIX       404    420
TURN        422    424
HELIX       425    428
HELIX       429    434
TURN        435    438
HELIX       442    453
HELIX       456    458
HELIX       462    469
TURN        470    472
STRAND      474    476
HELIX       478    492
STRAND      495    497
HELIX       500    511
HELIX       517    527
TURN        528    532
HELIX       536    541
HELIX       557    564
TURN        565    568
HELIX       571    574
HELIX       578    580
STRAND      712    715
HELIX       717    719
TURN        720    722
STRAND      723    732
STRAND      735    744
STRAND      746    748
STRAND      754    757
STRAND      765    768
STRAND      781    790
STRAND      802    808
STRAND      813    819
HELIX       822    825
HELIX       834    843
HELIX       846    848
STRAND      850    854
HELIX       861    870
STRAND      874    881
STRAND      884    893
STRAND      898    905
STRAND      910    920
HELIX       921    923
HELIX       924    936
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Nucleotide Sequence
Length: 5701 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 937 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
TGFBR2BioGRID 
BCR/ABLIntAct 
ACVR1HPRD 
SNAP91HPRD 
AP1M1IntAct 
AP1M2HPRD 
AP2A2HPRD 
Ap2a2IntAct 
AP1M1BioGRID 
AP1M2BioGRID 
AP2M1HPRD 
Ap2m1IntAct 
Ap2s1IntAct 
APCIntAct 
LDLRAP1HPRD 
ARRB1HPRD 
ARRB2BioGRID 
_BioGRID 
_HPRD 
MLH1BioGRID 
_BioGRID 
_BioGRID 
_HPRD 
BUB1BBioGRID 
BUB1HPRD 
DHX58HPRD 
MLH1BioGRID 
NECAP2BioGRID 
FAM46CHPRD 
gBIntAct 
TUBGCP4HPRD 
ITSN1IntAct 
KIAA0408HPRD 
MEA1BioGRID 
MLH1BioGRID 
NECAP2HPRD 
NUP54HPRD 
POM121HPRD 
_HPRD 
BUB1HPRD 
SMURF1HPRD 
_HPRD 
_BioGRID 
TGFBR1HPRD 
Ap2a2IntAct 
ATP6V1G1HPRD 
_MINT 
RIBC2HPRD 
RNPS1IntAct 
SMAD4HPRD 
SMURF1HPRD 
TERF2IPHPRD 
TGFBR1HPRD 
TGOLN2IntAct 
THAP1HPRD 
ATP6V1G1HPRD 
AP2M1IntAct 
Ap2m1IntAct 
AP2S1HPRD 
APCBioGRID 
LDLRAP1BioGRID 
ARRB2BioGRID 
ATMHPRD 
_BioGRID 
_BioGRID 
_BioGRID 
_BioGRID 
_HPRD 
BUB1BHPRD 
TGFBR2HPRD 
ACVR1HPRD 
RNPS1BioGRID 
DHX58IntAct 
CLINT1HPRD 
EPS15IntAct 
FAM46CIntAct 
gBMINT 
GRB2IntAct 
HIP1HPRD 
KIAA0408IntAct 
MEA1HPRD 
MLH1HPRD 
NECAP2IntAct 
NUP54IntAct 
OSBPL10BioGRID 
POM121IntAct 
PIP5K1CHPRD 
APCBioGRID 
_HPRD 
TGFBR1BioGRID 
y0291IntAct 
HIP1HPRD 
TUBGCP4IntAct 
_BioGRID 
RIBC2IntAct 
RNPS1BioGRID 
SHC1IntAct 
TERF2IPIntAct 
TGFBR1BioGRID 
TGFBR2HPRD 
THAP1IntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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