SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000409 
UniProt Accession
Theoretical PI
4.65  
Molecular Weight
53837 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
RNF14 
Gene Synonyms/Alias
ARA54 
Protein Name
E3 ubiquitin-protein ligase RNF14 
Protein Synonyms/Alias
EC=6.3.2.- Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein; 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:5;141337893-141369856;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
The decreased expression of ARA54 and increased expression of ARA55 is a feature of nonobstructive azoospermia. In addition, the differential localization of ARA54 may play an important role in testicular development and spermatogenesis in humans. 
The information of related literatures
1. K. C. Lan, C. Y. Hseh, S. Y. Lu, S. Y. Chang, C. R. Shyr, Y. T. Chen, H. Y. Kang and K. E. Huang (2008) Expression of androgen receptor co-regulators in the testes of men with azoospermia. Fertil Steril 89(5 Suppl): 1397-405. 

Abstract
OBJECTIVE PMID: [17919607] 

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Figures for illustrating the function of this protein/gene
Function
Might act as an E3 ubiquitin-protein ligase whichaccepts ubiquitin from specific E2 ubiquitin-conjugating enzymesand then transfers it to substrates, which could be nuclearproteins. Could play a role as a coactivator for androgen- and, toa lesser extent, progesterone-dependent transcription. 
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Subcellular Location
Cytoplasm. Nucleus. 
Tissue Specificity
Widely expressed. 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm IDA:UniProtKB.
GO:0005634 C:nucleus IDA:UniProtKB.
GO:0019787 F:small conjugating protein ligase activity IDA:UniProtKB.
GO:0003713 F:transcription coactivator activity NAS:UniProtKB.
GO:0008270 F:zinc ion binding IEA:InterPro.
GO:0030521 P:androgen receptor signaling pathway NAS:UniProtKB.
GO:0045893 P:positive regulation of transcription, DNA-dependent IDA:UniProtKB.
GO:0016567 P:protein ubiquitination IEP:UniProtKB.
GO:0060765 P:regulation of androgen receptor signaling pathway IDA:UniProtKB.
GO:0006357 P:regulation of transcription from RNA polymerase II promoter TAS:UniProtKB.
GO:0032355 P:response to estradiol stimulus IDA:BHF-UCL.
GO:0006351 P:transcription, DNA-dependent IEA:UniProtKB-KW.
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Interpro
IPR006575;    RWD-domain.
IPR016135;    UBQ-conjugating_enzyme/RWD.
IPR002867;    Znf_C6HC.
IPR001841;    Znf_RING.
IPR013083;    Znf_RING/FYVE/PHD.
IPR017907;    Znf_RING_CS.
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Pfam
PF01485;    IBR;    2.
PF05773;    RWD;    1.
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SMART
SM00647;    IBR;    2.
SM00184;    RING;    1.
SM00591;    RWD;    1.
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PROSITE
PS50908;    RWD;    1.
PS00518;    ZF_RING_1;    1.
PS50089;    ZF_RING_2;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    474       E3 ubiquitin-protein ligase RNF14.
                             /FTId=PRO_0000056057.
DOMAIN       11    137       RWD.
ZN_FING     220    270       RING-type 1; atypical.
ZN_FING     289    350       IBR-type.
ZN_FING     404    433       RING-type 2; degenerate.
REGION      361    474       Interaction with androgen receptor.
COILED      351    395       Potential.
MOTIF        37     45       D-box.
MOD_RES     348    348       Phosphoserine.
MUTAGEN     220    220       C->S: Loss of interaction with UBE2E2 and
                             of autoubiquitination.
CONFLICT     32     32       Q -> R (in Ref. 4; CAG32983).
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Nucleotide Sequence
Length: 3056 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 474 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
UBE2E1BioGRID 
RNF14BioGRID 
ARBioGRID 
UBE2E1BioGRID 
NR3C4BioGRID 
FAM46AHPRD 
UBE2WBioGRID 
FAM46AMINT 
PUBC1BioGRID 
_BioGRID 
RNF14BioGRID 
ARHPRD 
NR3C1HPRD 
NCOA4HPRD 
ESR1HPRD 
NR3C4BioGRID 
NR1i1BioGRID 
NR3C1HPRD 
NCOA4HPRD 
NCOA4HPRD 
_HPRD 
RNF14BioGRID 
RNF14BioGRID 
RNF6IntAct 
UBE2D4IntAct 
UBE2E1HPRD 
UBE2E3IntAct 
UBE2V1IntAct 
UBE2WIntAct 
VDRBioGRID 
UBE2D1BioGRID 
UBE2D2BioGRID 
UBE2D3BioGRID 
UBE2D4BioGRID 
UBE2E1BioGRID 
UBE2E2BioGRID 
UBE2E3BioGRID 
UBE2V1BioGRID 
UBE2UBioGRID 
UBE2WBioGRID 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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