SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000411 
UniProt Accession
Theoretical PI
5.23  
Molecular Weight
210512 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
AKAP11 
Gene Synonyms/Alias
AKAP220, KIAA0629 
Protein Name
A-kinase anchor protein 11 
Protein Synonyms/Alias
AKAP-11 A-kinase anchor protein 220 kDa;AKAP 220hAKAP220 Protein kinase A-anchoring protein 11;PRKA11 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:13;42846289-42897396;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Using a combination of protein kinase A type II overlay screening, rapid amplification of cDNA ends. 
The information of related literatures
1. N. Reinton, P. Collas, T. B. Haugen, B. S. Skalhegg, V. Hansson, T. Jahnsen and K. Tasken (2000) Localization of a novel human A-kinase-anchoring protein, hAKAP220, during spermatogenesis. Dev Biol 223(1): 194-204. 

Abstract
Using a combination of protein kinase A type II overlay screening, rapid amplification of cDNA ends, and database searches, a contig of 9923 bp was assembled and characterized in which the open reading frame encoded a 1901-amino-acid A-kinase-anchoring protein (AKAP) with an apparent SDS-PAGE mobility of 220 kDa, named human AKAP220 (hAKAP220). The hAKAP220 amino acid sequence revealed high similarity to rat AKAP220 in the 1167 C-terminal residues, but contained 727 residues in the N-terminus not present in the reported rat AKAP220 sequence. The hAKAP220 mRNA was expressed at high levels in human testis and in isolated human pachytene spermatocytes and round spermatids. The hAKAP220 protein was present in human male germ cells and mature sperm. Immunofluorescent labeling with specific antibodies indicated that hAKAP220 was localized in the cytoplasm of premeiotic pachytene spermatocytes and in the centrosome of developing postmeiotic germ cells, while a midpiece/centrosome localization was found in elongating spermatocytes and mature sperm. The hAKAP220 protein together with a fraction of PKA types I and II and protein phosphatase I was resistant to detergent extraction of sperm tails, suggesting an association with cytoskeletal structures. In contrast, S-AKAP84/D-AKAP1, which is also present in the midpiece, was extracted under the same conditions. Anti-hAKAP220 antisera coimmunoprecipitated both type I and type II regulatory subunits of PKA in human testis lysates, indicating that hAKAP220 interacts with both classes of R subunits, either through separate or through a common binding motif(s). PMID: [10864471] 

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Figures for illustrating the function of this protein/gene
Function
Binds to type II regulatory subunits of protein kinase Aand anchors/targets them. 
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Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton,centrosome. Note=Cytoplasmic in premeiotic pachytene spermatocytesand in the centrosome of developing postmeiotic germ cells, whilea midpiece/centrosome localization was found in elongatingspermatocytes and mature sperm. 
Tissue Specificity
Expressed in heart, brain, lung, liver,kidney, testis and ovary. Weakly expressed in skeletal muscle,pancreas and spleen. 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm IDA:HPA.
GO:0005815 C:microtubule organizing center IEA:UniProtKB-SubCell.
GO:0005730 C:nucleolus IDA:HPA.
GO:0005886 C:plasma membrane IDA:HPA.
GO:0043234 C:protein complex IEA:Compara.
GO:0007243 P:intracellular protein kinase cascade TAS:ProtInc.
GO:0010738 P:regulation of protein kinase A signaling cascade IEA:Compara.
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Interpro
IPR008382;    SPHK1-interactor_AKAP_110.
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Pfam
SMART
PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   1901       A-kinase anchor protein 11.
                             /FTId=PRO_0000064517.
REGION     1650   1663       PKA-RII subunit binding domain.
COMPBIAS    238    315       Ser-rich.
COMPBIAS   1705   1767       Ser-rich.
MOD_RES      18     18       Phosphoserine.
MOD_RES     422    422       Phosphoserine.
MOD_RES     433    433       Phosphoserine.
MOD_RES     448    448       Phosphoserine.
MOD_RES    1100   1100       Phosphothreonine.
MOD_RES    1104   1104       Phosphothreonine.
MOD_RES    1169   1169       Phosphoserine.
MOD_RES    1240   1240       Phosphoserine.
MOD_RES    1242   1242       Phosphoserine.
MOD_RES    1337   1337       Phosphoserine.
MOD_RES    1340   1340       Phosphoserine.
VARIANT     721    721       S -> C (in dbSNP:rs2236364).
                             /FTId=VAR_020131.
VARIANT    1070   1070       H -> R (in dbSNP:rs17063163).
                             /FTId=VAR_048207.
VARIANT    1410   1410       L -> F (in dbSNP:rs17063167).
                             /FTId=VAR_048208.
CONFLICT   1303   1303       K -> R (in Ref. 5; BAA92117).
CONFLICT   1444   1469       LDPYRNEVSQLYSFSTSLVHSITKDA -> CGPSVELSPWK
                             WQTRCGREGNSWKRE (in Ref. 5; BAA92117).
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Nucleotide Sequence
Length: 9923 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 1901 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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