SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000414 
UniProt Accession
Theoretical PI
6.18  
Molecular Weight
172581 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
LRRC7 
Gene Synonyms/Alias
KIAA1365, LAP1 
Protein Name
Leucine-rich repeat-containing protein 7 
Protein Synonyms/Alias
Densin-180;Densin Protein LAP1; 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:1;70034081-70589164;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Densin is a junction protein suggested to play a role in establishment of specific cell-cell contacts in the post-synaptic densities of the brain and the slit diaphragm of the kidney podocyte.  
The information of related literatures
1. M. Lassila, J. Juhila, E. Heikkila and H. Holthofer (2007) Densin is a novel cell membrane protein of Sertoli cells in the testis. Mol Reprod Dev 74(5): 641-5. 

Abstract
Cell-cell interactions between Sertoli cells and germ cells are crucial for the maturation of germ cells in spermatogenesis but the structural and functional aspects of the interactions remain to be fully elucidated. Densin is a junction protein suggested to play a role in establishment of specific cell-cell contacts in the post-synaptic densities of the brain and the slit diaphragm of the kidney podocyte. In the present study, densin was discovered to be expressed in the testis of the man and the mouse. Expression of densin at the gene and the protein level was studied by using RT-PCR and Western blotting analyses, and the localization of densin was explored with immunofluorescence staining. RT-PCR and Western blotting analyses showed that densin is expressed at the gene and the protein levels. Immunofluorescence staining localized the expression of densin to the cell membranes of Sertoli cells suggesting that densin may be an adherens junction protein between Sertoli cells and developing germ cells. Densin is a novel testicular protein expressed in the cell membranes of Sertoli cells. Its functional role remains to be assessed. PMID: [17039495] 

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Figures for illustrating the function of this protein/gene
Ref: M. Lassila, J. Juhila, E. Heikkila and H. Holthofer (2007) Densin is a novel cell membrane protein of Sertoli cells in the testis. Mol Reprod Dev 74(5): 641-5. PMID: [17039495]
Ref: M. Lassila, J. Juhila, E. Heikkila and H. Holthofer (2007) Densin is a novel cell membrane protein of Sertoli cells in the testis. Mol Reprod Dev 74(5): 641-5. PMID: [17039495]
Function
Required for normal synaptic spine architecture andfunction. Necessary for DISC1 and GRM5 localization topostsynaptic density complexes and for both N-methyl D-aspartatereceptor-dependent and metabotropic glutamate receptor-dependentlong term depression. 
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Subcellular Location
Cytoplasm. Cell junction, synapse,postsynaptic cell membrane, postsynaptic density (By similarity). 
Tissue Specificity
Brain-specific. Isoform 3 is ubiquitouslyexpressed. 
Gene Ontology
GO IDGO termEvidence
GO:0030054 C:cell junction IEA:UniProtKB-KW.
GO:0005737 C:cytoplasm IEA:UniProtKB-SubCell.
GO:0005886 C:plasma membrane IEA:UniProtKB-KW.
GO:0014069 C:postsynaptic density IEA:UniProtKB-SubCell.
GO:0045211 C:postsynaptic membrane IEA:UniProtKB-KW.
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Interpro
IPR001611;    Leu-rich_rpt.
IPR025875;    Leu-rich_rpt_2_copies.
IPR001478;    PDZ.
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Pfam
PF00560;    LRR_1;    1.
PF12799;    LRR_4;    2.
PF00595;    PDZ;    1.
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SMART
SM00228;    PDZ;    1.
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PROSITE
PS51450;    LRR;    15.
PS50106;    PDZ;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1   1537       Leucine-rich repeat-containing protein 7.
                             /FTId=PRO_0000188298.
REPEAT       23     44       LRR 1.
REPEAT       47     68       LRR 2.
REPEAT       70     91       LRR 3.
REPEAT       93    114       LRR 4.
REPEAT      116    137       LRR 5.
REPEAT      139    161       LRR 6.
REPEAT      162    183       LRR 7.
REPEAT      185    206       LRR 8.
REPEAT      208    229       LRR 9.
REPEAT      231    253       LRR 10.
REPEAT      254    275       LRR 11.
REPEAT      277    298       LRR 12.
REPEAT      300    321       LRR 13.
REPEAT      323    344       LRR 14.
REPEAT      346    367       LRR 15.
REPEAT      369    391       LRR 16.
REPEAT      392    413       LRR 17.
DOMAIN     1445   1535       PDZ.
MOD_RES      90     90       Phosphoserine (By similarity).
MOD_RES     850    850       Phosphoserine (By similarity).
MOD_RES     865    865       Phosphothreonine (By similarity).
MOD_RES     933    933       Phosphoserine (By similarity).
MOD_RES     949    949       Phosphoserine (By similarity).
MOD_RES    1439   1439       Phosphoserine (By similarity).
VAR_SEQ     178    179       KS -> NI (in isoform 3).
                             /FTId=VSP_010795.
VAR_SEQ     180   1537       Missing (in isoform 3).
                             /FTId=VSP_010796.
VAR_SEQ    1286   1332       Missing (in isoform 2).
                             /FTId=VSP_010797.
VARIANT     235    235       L -> M (in a breast cancer sample;
                             somatic mutation).
                             /FTId=VAR_035715.
VARIANT    1054   1054       H -> Y (in dbSNP:rs12069888).
                             /FTId=VAR_049727.
CONFLICT    373    373       L -> P (in Ref. 4).
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Nucleotide Sequence
Length: 5000 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 1537 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Other Protein-Protein interaction resources
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