SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00000415 
UniProt Accession
Theoretical PI
6.88  
Molecular Weight
71836 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
PIAS1 
Gene Synonyms/Alias
DDXBP1 
Protein Name
E3 SUMO-protein ligase PIAS1 
Protein Synonyms/Alias
EC=6.3.2.- DEAD/H box-binding protein 1; Gu-binding protein;GBP Protein inhibitor of activated STAT protein 1; RNA helicase II-binding protein; 
Organism
Homo sapiens (Human) 
NCBI Taxonomy ID
9606 
Chromosome Location
chr:15;68346572-68480402;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Immunostaining of human and mouse testis with PIAS1-specific antiserum demonstrated coexpression of PIAS1 with AR in Sertoli cells and Leydig cells. In addition, PIAS1 was expressed in spermatogenic cells;PIAS1 functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis.  
The information of related literatures
1. J. Tan, S. H. Hall, K. G. Hamil, G. Grossman, P. Petrusz, J. Liao, K. Shuai and F. S. French (2000) Protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1) is a nuclear receptor coregulator expressed in human testis. Mol Endocrinol 14(1): 14-26. 

Abstract
An androgen receptor (AR) interacting protein was isolated from a HeLa cell cDNA library by two-hybrid screening in yeast using the AR DNA+ligand binding domains as bait. The protein has sequence identity with human protein inhibitor of activated signal transducer and activator of transcription (PIAS1) and human Gu RNA helicase II binding protein (GBP). Binding of PIAS1 to human AR DNA+ligand binding domains was androgen dependent in the yeast liquid beta-galactosidase assay. Activation of binding by dihydrotestosterone was greater than testosterone > estradiol > progesterone. PIAS1 binding to full-length human AR in a reversed yeast two hybrid system was also androgen dependent. [35S] PIAS1 bound a glutathione S-transferase-AR-DNA binding domain (amino acids 544-634) fusion protein in affinity matrix assays. In transient cotransfection assays using CV1 cells with full-length human AR and a mouse mammary tumor virus luciferase reporter vector, there was an androgen-dependent 3- to 5-fold greater increase in luciferase activity with PIAS1 over that obtained with an equal amount of control antisense cDNA or mutant PIAS1. Constitutive transcriptional activity of the AR N-terminal+DNA binding domain was increased 6-fold by PIAS1. PIAS1 also enhanced glucocorticoid receptor transactivation in response to dexamethasone but inhibited progesterone-induced progesterone receptor transactivation in the same assay system. mRNA for PIAS1 was highly expressed in testis of human, monkey, rat, and mouse. In rat testis the onset of PIAS1 mRNA expression coincided with the initiation of spermatogenesis between 25-30 days of age. Immunostaining of human and mouse testis with PIAS1-specific antiserum demonstrated coexpression of PIAS1 with AR in Sertoli cells and Leydig cells. In addition, PIAS1 was expressed in spermatogenic cells. The results suggest that PIAS1 functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. PMID: [10628744] 

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Figures for illustrating the function of this protein/gene
Ref: J. Tan, S. H. Hall, K. G. Hamil, G. Grossman, P. Petrusz, J. Liao, K. Shuai and F. S. French (2000) Protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1) is a nuclear receptor coregulator expressed in human testis. Mol Endocrinol 14(1): 14-26. PMID: [10628744]
Function
Functions as an E3-type small ubiquitin-like modifier(SUMO) ligase, stabilizing the interaction between UBE2I and thesubstrate, and as a SUMO-tethering factor. Plays a crucial role asa transcriptional coregulation in various cellular pathways,including the STAT pathway, the p53 pathway and the steroidhormone signaling pathway. In vitro, binds A/T-rich DNA. Theeffects of this transcriptional coregulation, transactivation orsilencing, may vary depending upon the biological context.Together with PRMT1, may repress STAT1 transcriptional activity,in the late phase of interferon gamma (IFN-gamma) signaling. 
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Subcellular Location
Nucleus speckle. Note=Interaction with CSRP2may induce a partial redistribution along the cytoskeleton. 
Tissue Specificity
Expressed in numerous tissues with highestlevel in testis. 
Gene Ontology
GO IDGO termEvidence
GO:0016607 C:nuclear speck IEA:UniProtKB-SubCell.
GO:0005654 C:nucleoplasm TAS:Reactome.
GO:0003677 F:DNA binding IEA:UniProtKB-KW.
GO:0019789 F:SUMO ligase activity IDA:BHF-UCL.
GO:0003713 F:transcription coactivator activity NAS:UniProtKB.
GO:0003714 F:transcription corepressor activity TAS:ProtInc.
GO:0008270 F:zinc ion binding IEA:InterPro.
GO:0030521 P:androgen receptor signaling pathway NAS:UniProtKB.
GO:0060333 P:interferon-gamma-mediated signaling pathway TAS:Reactome.
GO:0007259 P:JAK-STAT cascade TAS:ProtInc.
GO:0033235 P:positive regulation of protein sumoylation IDA:UniProtKB.
GO:0045893 P:positive regulation of transcription, DNA-dependent NAS:UniProtKB.
GO:0016925 P:protein sumoylation IEA:UniProtKB-UniPathway.
GO:0060334 P:regulation of interferon-gamma-mediated signaling pathway TAS:Reactome.
GO:0006351 P:transcription, DNA-dependent IEA:UniProtKB-KW.
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Interpro
IPR023321;    PINIT.
IPR003034;    SAP_DNA-bd.
IPR004181;    Znf_MIZ.
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Pfam
PF14324;    PINIT;    1.
PF02891;    zf-MIZ;    1.
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SMART
SM00513;    SAP;    1.
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PROSITE
PS51466;    PINIT;    1.
PS50800;    SAP;    1.
PS51044;    ZF_SP_RING;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    651       E3 SUMO-protein ligase PIAS1.
                             /FTId=PRO_0000218974.
DOMAIN       11     45       SAP.
DOMAIN      124    288       PINIT.
REPEAT      520    523       1.
REPEAT      557    560       2.
REPEAT      598    601       3; approximate.
REPEAT      612    615       4; approximate.
ZN_FING     320    397       SP-RING-type.
REGION      462    473       SUMO1-binding (By similarity).
REGION      520    615       4 X 4 AA repeats of N-T-S-L.
MOTIF        19     23       LXXLL motif.
MOTIF        56     64       Nuclear localization signal (Potential).
MOTIF       368    380       Nuclear localization signal (Potential).
COMPBIAS    577    634       Ser-rich.
MOD_RES     303    303       Asymmetric dimethylarginine; by PRMT1.
MOD_RES     466    466       Phosphoserine.
MOD_RES     467    467       Phosphoserine.
MOD_RES     468    468       Phosphoserine.
MOD_RES     483    483       Phosphoserine (By similarity).
MOD_RES     485    485       Phosphoserine (By similarity).
MOD_RES     487    487       Phosphothreonine (By similarity).
MOD_RES     503    503       Phosphoserine.
MOD_RES     510    510       Phosphoserine.
MOD_RES     522    522       Phosphoserine.
MUTAGEN     303    303       R->K: Partial loss of repression on STAT1
                             transcriptional activity.
MUTAGEN     351    351       C->A,S: Loss of UBE2I-binding; almost
                             complete loss of promotion of TP53
                             sumoylation; no loss of SUMO1- and TP53-
                             binding.
CONFLICT    119    119       E -> K (in Ref. 1; AAC36702).
CONFLICT    266    268       IVV -> MC (in Ref. 1; AAC36702).
CONFLICT    613    613       S -> T (in Ref. 5; AAB58488).
HELIX         6     11
HELIX        16     24
HELIX        34     46
HELIX        51     63
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Nucleotide Sequence
Length: 1953 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 651 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
_BioGRID 
SUMO1BioGRID 
PIG59BioGRID 
AKT1BioGRID 
ARBioGRID 
AKT1BioGRID 
_BioGRID 
_BioGRID 
_BioGRID 
_BioGRID 
_BioGRID 
_BioGRID 
_BioGRID 
_BioGRID 
SP3BioGRID 
BRCA1BioGRID 
CASP8HPRD 
CREBBPHPRD 
CEBPAHPRD 
CREB1BioGRID 
CSRP2BioGRID 
PPARGBioGRID 
_BioGRID 
DNMT3ABioGRID 
MBD1BioGRID 
DNMT3ABioGRID 
MITFBioGRID 
BRCA1BioGRID 
NCOR1BioGRID 
ESR1DIP 
ESR2HPRD 
NR3C4BioGRID 
GLULBioGRID 
GRM8HPRD 
CHUKHPRD 
MBD1HPRD 
MITFBioGRID 
MSX1HPRD 
MX1BioGRID 
NCOR1BioGRID 
NINHPRD 
TP53BioGRID 
SERBP1HPRD 
PTPN1HPRD 
SUMO1BioGRID 
ARHPRD 
AXIN1HPRD 
NR3C2HPRD 
SNIP1HPRD 
SP3BioGRID 
BRCA1BioGRID 
CEBPEHPRD 
NR2F2HPRD 
CSRP2HPRD 
PPARGBioGRID 
DDX21HPRD 
DNMT3AHPRD 
DNM1HPRD 
DNMT3ABioGRID 
PRPF40AHPRD 
BRCA1BioGRID 
NCOR1BioGRID 
ELK3HPRD 
ESR1HPRD 
ESR2DIP 
NR2F2HPRD 
NR5A1HPRD 
GATA4HPRD 
GLULIntAct 
JUNHPRD 
MBD1MINT 
NR3C2HPRD 
MDM2HPRD 
NCOR1BioGRID 
TP53HPRD 
TP73HPRD 
PIAS1HPRD 
PIAS1HPRD 
PIAS2HPRD 
PIAS4HPRD 
PPARGBioGRID 
PRPF40AHPRD 
PGRDIP 
PTPN1HPRD 
PRPF40AHPRD 
BRCA1BioGRID 
BRCA1BioGRID 
_HPRD 
_BioGRID 
_HPRD 
DKFZp666O0110HPRD 
BRCA1BioGRID 
NCOR1BioGRID 
SP3HPRD 
_BioGRID 
DNMT3ABioGRID 
_HPRD 
RBBP6IntAct 
SATB2HPRD 
SIAH2BioGRID 
SKILHPRD 
SMAD1HPRD 
SMAD4HPRD 
SMAD7HPRD 
SNIP1HPRD 
SP3HPRD 
STAB2BioGRID 
STAT1HPRD 
NR5A1HPRD 
SUFUHPRD 
SUMO1HPRD 
RELAHPRD 
UBE2IHPRD 
E1MINT 
E1MINT 
ZMIZ2BioGRID 
ZNF76HPRD 
PPARGBioGRID 
PRPF40AMINT 
PGRHPRD 
PTPN1IntAct 
BRCA1BioGRID 
TBPHPRD 
BRCA1BioGRID 
CREBBPHPRD 
_BioGRID 
CREB1BioGRID 
_BioGRID 
NINHPRD 
CREB1BioGRID 
NINHPRD 
BRCA1BioGRID 
NCOR1BioGRID 
ESR2HPRD 
SP3BioGRID 
_BioGRID 
DKFZp434N1610BioGRID 
DNMT3ABioGRID 
MITF-CBioGRID 
_BioGRID 
SIAH1BioGRID 
SIAH2BioGRID 
SKILMINT 
SMAD1MINT 
SMAD4MINT 
SMAD7MINT 
SNIP1MINT 
SP3BioGRID 
SREBF2BioGRID 
SUMO1BioGRID 
SUMO2BioGRID 
SUMO3BioGRID 
TBPHPRD 
TRIM27BioGRID 
UBE2IBioGRID 
E1MINT 
E1MINT 
ZNF76BioGRID 
Other Protein-Protein interaction resources
String database  
View Microarray data
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