SG00000419

SpermatogenesisOnline 1.0

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Content

SG ID

SG00000419

UniProt Accession

GRP78_HUMAN;P11021;B0QZ61;Q2EF78;Q9NPF1;Q9UK02;

Theoretical PI

5.01

Molecular Weight

72333 Da

Genbank Nucleotide ID

M19645; X87949; AJ271729; AF216292; DQ385847; AL354710; CH471090; BC020235; X59969; AF188611;

Genbank Protein ID

AAA52614.1; CAA61201.1; CAB71335.1; AAF42836.1; ABD04090.1; CAQ08732.1; EAW87620.1; AAH20235.1; CAA42595.1; AAF13605.1;

Gene Name

HSPA5

Gene Synonyms/Alias

GRP78

Protein Name

78 kDa glucose-regulated protein

Protein Synonyms/Alias

GRP-78 Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein;BiPFlags: Precursor

Organism

Homo sapiens (Human)

NCBI Taxonomy ID

9606

Chromosome Location

chr:9;127997132-128003622;-1

View in Ensembl genome browser

Function in Stage

postmeiotic

meiotic

Function in Cell Type

spermatocyte

spermatid

Description

These results indicate that in addition to being expressed in human testis spermatogenic cells, both Hsp60 and Grp78 proteins persist in ejaculated spermatozoa. These findings are in agreement with the involvement of Hsp60 and Grp78 during spermatogenesis and in sperm functions such as fertilization.

The information of related literatures

1. C. Lachance, M. Fortier, V. Thimon, R. Sullivan, J. L. Bailey and P. Leclerc (2010) Localization of Hsp60 and Grp78 in the human testis, epididymis and mature spermatozoa. Int J Androl 33(1): 33-44.

Abstract
Molecular chaperones of the heat shock proteins (HSP) family are important in numerous cellular processes. In this study, the expression of Hsp60 and Grp78 proteins was investigated in the male reproductive tract. The cellular distribution of Hsp60 and Grp78 proteins was analysed in the human testis and epididymis by immunohistochemical approaches. DNA microarray technology was used to analyse HSP60 and GRP78 gene expression along human epididymis. The cellular localization of these chaperone proteins in ejaculated spermatozoa was investigated by indirect immunofluorescence and by Western blot following sperm sub-cellular fractionation. In the human testis, Hsp60 was detected in spermatogonia, whereas a strong Grp78 staining was observed in spermatocytes and round spermatids. Grp78 protein was also observed in the epididymal epithelium, whereas no Hsp60 staining was observed in this organ by immunohistochemistry. The presence of both Hsp60 and Grp78 RNA in human epididymis was confirmed by microarrays. In ejaculated spermatozoa, Hsp60 was localized in the mid-piece, whereas Grp78 was detected in the neck region. These results indicate that in addition to being expressed in human testis spermatogenic cells, both Hsp60 and Grp78 proteins persist in ejaculated spermatozoa. These findings are in agreement with the involvement of Hsp60 and Grp78 during spermatogenesis and in sperm functions such as fertilization. PMID: [19207617]

Figures for illustrating the function of this protein/gene

	Ref: C. Lachance, M. Fortier, V. Thimon, R. Sullivan, J. L. Bailey and P. Leclerc (2010) Localization of Hsp60 and Grp78 in the human testis, epididymis and mature spermatozoa. Int J Androl 33(1): 33-44. PMID: [19207617]
	Ref: C. Lachance, M. Fortier, V. Thimon, R. Sullivan, J. L. Bailey and P. Leclerc (2010) Localization of Hsp60 and Grp78 in the human testis, epididymis and mature spermatozoa. Int J Androl 33(1): 33-44. PMID: [19207617]
	Ref: C. Lachance, M. Fortier, V. Thimon, R. Sullivan, J. L. Bailey and P. Leclerc (2010) Localization of Hsp60 and Grp78 in the human testis, epididymis and mature spermatozoa. Int J Androl 33(1): 33-44. PMID: [19207617]

Function

Probably plays a role in facilitating the assembly ofmultimeric protein complexes inside the ER.

Subcellular Location

Endoplasmic reticulum lumen. Melanosome.Note=Identified by mass spectrometry in melanosome fractions fromstage I to stage IV.

Tissue Specificity

Gene Ontology

GO ID	GO term	Evidence
GO:0009986	C:cell surface	IDA:UniProtKB.
GO:0034663	C:endoplasmic reticulum chaperone complex	IDA:UniProtKB.
GO:0005788	C:endoplasmic reticulum lumen	TAS:UniProtKB.
GO:0005793	C:endoplasmic reticulum-Golgi intermediate compartment	IDA:UniProtKB.
GO:0030176	C:integral to endoplasmic reticulum membrane	IDA:UniProtKB.
GO:0042470	C:melanosome	IEA:UniProtKB-SubCell.
GO:0030496	C:midbody	IDA:UniProtKB.
GO:0005634	C:nucleus	IDA:UniProtKB.
GO:0048471	C:perinuclear region of cytoplasm	IDA:UniProtKB.
GO:0005524	F:ATP binding	NAS:UniProtKB.
GO:0016887	F:ATPase activity	ISS:UniProtKB.
GO:0005509	F:calcium ion binding	TAS:UniProtKB.
GO:0043027	F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process	IDA:UniProtKB.
GO:0043022	F:ribosome binding	IEA:Compara.
GO:0006987	P:activation of signaling protein activity involved in unfolded protein response	TAS:Reactome.
GO:0006916	P:anti-apoptosis	IMP:UniProtKB.
GO:0042149	P:cellular response to glucose starvation	IDA:UniProtKB.
GO:0021680	P:cerebellar Purkinje cell layer development	IEA:Compara.
GO:0021589	P:cerebellum structural organization	IEA:Compara.
GO:0006983	P:ER overload response	IEA:Compara.
GO:0030433	P:ER-associated protein catabolic process	TAS:BHF-UCL.
GO:0030512	P:negative regulation of transforming growth factor beta receptor signaling pathway	IEA:Compara.
GO:0030168	P:platelet activation	TAS:Reactome.
GO:0002576	P:platelet degranulation	TAS:Reactome.
GO:0031398	P:positive regulation of protein ubiquitination	IEA:Compara.
GO:0060904	P:regulation of protein folding in endoplasmic reticulum	TAS:BHF-UCL.

Interpro

IPR018181; Heat_shock_70_CS.
IPR013126; Hsp_70_fam.
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Pfam

PF00012; HSP70; 1.
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SMART

PROSITE

PS00014;    ER_TARGET;    1.
PS00297;    HSP70_1;    1.
PS00329;    HSP70_2;    1.
PS01036;    HSP70_3;    1.
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PRINTS

PR00301; HEATSHOCK70.;
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Created Date

18-Oct-2012

Record Type

Experiment identified

Protein sequence Annotation

SIGNAL        1     18
CHAIN        19    654       78 kDa glucose-regulated protein.
                             /FTId=PRO_0000013566.
MOTIF       651    654       Prevents secretion from ER.
MOD_RES     166    166       Phosphothreonine (By similarity).
MOD_RES     268    268       N6-acetyllysine.
MOD_RES     466    466       Phosphotyrosine.
MOD_RES     571    571       Phosphoserine (By similarity).
VARIANT     543    543       N -> H (in dbSNP:rs35356639).
                             /FTId=VAR_025815.
CONFLICT    297    297       Missing (in Ref. 1 and 2).
CONFLICT    418    418       D -> H (in Ref. 1 and 2).
CONFLICT    439    439       R -> S (in Ref. 1 and 2).
CONFLICT    447    447       K -> N (in Ref. 1 and 2).
STRAND       31     34
STRAND       37     46
STRAND       49     52
STRAND       60     63
STRAND       66     68
STRAND       74     76
HELIX        78     82
HELIX        84     86
HELIX        88     90
STRAND       91     93
HELIX        95     97
TURN         98    100
HELIX       106    114
STRAND      116    122
STRAND      125    131
STRAND      137    140
HELIX       142    161
STRAND      167    172
HELIX       178    190
STRAND      194    200
HELIX       201    208
HELIX       211    213
STRAND      215    225
STRAND      230    238
STRAND      241    250
HELIX       255    273
HELIX       278    280
HELIX       282    298
TURN        299    301
STRAND      302    313
STRAND      316    323
HELIX       324    336
HELIX       339    348
HELIX       353    355
STRAND      358    363
HELIX       364    367
HELIX       369    378
TURN        379    381
TURN        390    392
HELIX       393    406
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Nucleotide Sequence

Length: 5470 bp Go to nucleotide: FASTA

Protein Sequence

Length: 654 bp Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
1433B_HUMAN	YWHAB	HPRD
1433Z_HUMAN	YWHAZ	MINT
1C05_HUMAN	HLA-C	BioGRID
1C06_HUMAN	HLA-C	BioGRID
1C07_HUMAN	HLA-C	HPRD
1C12_HUMAN	HLA-C	BioGRID
2ABB_HUMAN	PPP2R2B	IntAct
5HT3A_HUMAN	HTR3A	HPRD
A2BF24_HUMAN	HLA-C	BioGRID
A2BF25_HUMAN	HLA-C	BioGRID
AAPK1_HUMAN	PRKAA1	IntAct
AGO4_HUMAN	EIF2C4	IntAct
AIRE_HUMAN	AIRE	BioGRID
APC_HUMAN	APC	BioGRID
ARRB1_HUMAN	ARRB1	IntAct
ARRB2_HUMAN	ARRB2	IntAct
B0LPF3_HUMAN	GRB2	BioGRID
B0UY14_HUMAN	HLA-C	BioGRID
B0UY15_HUMAN	HLA-C	BioGRID
B2RBL9_HUMAN	_	BioGRID
B2RP50_HUMAN	AIRE	BioGRID
B3KP86_HUMAN	_	BioGRID
B3KQW7_HUMAN	_	HPRD
B3KWD7_HUMAN	_	BioGRID
B3KWE2_HUMAN	_	BioGRID
B3KY45_HUMAN	_	HPRD
B4DEV4_HUMAN	_	BioGRID
B4DIW5_HUMAN	_	BioGRID
B4E398_HUMAN	_	HPRD
B7Z2B6_HUMAN	_	BioGRID
B7Z7X7_HUMAN	_	BioGRID
B7Z8N4	_	HPRD
BCAR1_HUMAN	BCAR1	BioGRID
BRAF_HUMAN	BRAF	IntAct
CASP7_HUMAN	CASP7	HPRD
CASP8_HUMAN	CASP8	IntAct
CAV1_HUMAN	CAV1	IntAct
CDK9_HUMAN	CDK9	BioGRID
CFTR_HUMAN	CFTR	IntAct
CH60_HUMAN	HSPD1	IntAct
CSN5_HUMAN	COPS5	IntAct
D6W5V7_HUMAN	BCDIN3	BioGRID
DDX24_HUMAN	DDX24	BioGRID
DJC10_HUMAN	DNAJC10	HPRD
DNJC1_HUMAN	DNAJC1	HPRD
DNJC3_MOUSE	Dnajc3	MINT
DPH1_HUMAN	DPH1	HPRD
DPYL5_HUMAN	DPYSL5	MINT
E2AK3_HUMAN	EIF2AK3	HPRD
E7ERM1_HUMAN	HLA-C	BioGRID
E9PCL5_HUMAN	BCAR1	BioGRID
E9PCV2_HUMAN	BCAR1	BioGRID
EBNA5_EBVB9	EBNA-LP	MINT
EGFR_HUMAN	EGFR	IntAct
EPM2A_HUMAN	EPM2A	BioGRID
ERN1_HUMAN	ERN1	HPRD
ERP29_HUMAN	ERP29	HPRD
ESR1_HUMAN	ESR1	IntAct
FA8_HUMAN	F8	HPRD
FCSD2_HUMAN	FCHSD2	IntAct
GBRAP_HUMAN	GABARAP	IntAct
GBRL1_HUMAN	GABARAPL1	IntAct
GRB2_HUMAN	GRB2	BioGRID
GRIA1_HUMAN	GRIA1	HPRD
A0PJU7_HUMAN	TSHR	BioGRID
A2MG_HUMAN	A2M	HPRD
APOB_HUMAN	APOB	HPRD
B2RBL9_HUMAN	_	HPRD
B3KQW7_HUMAN	_	BioGRID
B4DII3_HUMAN	_	BioGRID
B4DIW5_HUMAN	_	HPRD
B4DJZ8_HUMAN	_	BioGRID
B4DR00_HUMAN	_	BioGRID
B4DTQ3_HUMAN	_	BioGRID
BCAR1_HUMAN	BCAR1	HPRD
CASP7_HUMAN	CASP7	BioGRID
D3DR86_HUMAN	NFKB2	IntAct
DDX24_HUMAN	DDX24	HPRD
DJB11_HUMAN	DNAJB11	MINT
DJC10_HUMAN	DNAJC10	BioGRID
DPH1_HUMAN	DPH1	BioGRID
DPYL5_HUMAN	DPYSL5	HPRD
E2AK3_HUMAN	EIF2AK3	IntAct
ERN1_HUMAN	ERN1	BioGRID
GRB2_HUMAN	GRB2	IntAct
IGHM_HUMAN	IGHM	HPRD
IKBA_HUMAN	NFKBIA	IntAct
IKBB_HUMAN	NFKBIB	IntAct
IKBE_HUMAN	NFKBIE	IntAct
IKKB_HUMAN	IKBKB	IntAct
K2C8_HUMAN	KRT8	BioGRID
LDLR_HUMAN	LDLR	BioGRID
M3K1_HUMAN	MAP3K1	IntAct
M3K3_HUMAN	MAP3K3	IntAct
M3K8_HUMAN	MAP3K8	IntAct
MYOME_HUMAN	PDE4DIP	String
NDRG1_HUMAN	NDRG1	IntAct
NEMO_HUMAN	IKBKG	IntAct
NFKB2_HUMAN	NFKB2	IntAct
O43318-2	MAP3K7	IntAct
PAWR_HUMAN	PAWR	IntAct
PDIP3_HUMAN	POLDIP3	String
PSME3_HUMAN	PSME3	HPRD
PTEN_HUMAN	PTEN	IntAct
Q04206-2	RELA	IntAct
Q59FQ1_HUMAN	_	BioGRID
Q59HB3_HUMAN	_	HPRD
Q6ZUH1_HUMAN	_	IntAct
Q7Z7Q0_HUMAN	APOB	HPRD
Q8TB90	TSHR	BioGRID
RAF1_HUMAN	RAF1	IntAct
RELB_HUMAN	RELB	IntAct
REL_HUMAN	REL	IntAct
RIPK1_HUMAN	RIPK1	IntAct
RIPK3_HUMAN	RIPK3	IntAct
SH3B4_HUMAN	SH3BP4	IntAct
SIL1_HUMAN	SIL1	BioGRID
TAB1_HUMAN	TAB1	IntAct
TAB2_HUMAN	TAB2	IntAct
TANK_HUMAN	TANK	IntAct
TBK1_HUMAN	TBK1	IntAct
THYG_HUMAN	TG	BioGRID
TMM62_HUMAN	TMEM62	HPRD
TNR1A_HUMAN	TNFRSF1A	IntAct
TNR1B_HUMAN	TNFRSF1B	IntAct
TRAF2_HUMAN	TRAF2	IntAct
TRAF6_HUMAN	TRAF6	IntAct
TSHR_HUMAN	TSHR	BioGRID
H2AX_HUMAN	H2AFX	BioGRID
HFE_HUMAN	HFE	MINT
HPBP1_HUMAN	HSPBP1	HPRD
IKKB_HUMAN	IKBKB	MINT
K1C18_HUMAN	KRT18	HPRD
K2C8_HUMAN	KRT8	HPRD
LDLR_HUMAN	LDLR	HPRD
LPH_HUMAN	LCT	HPRD
M3K14_HUMAN	MAP3K14	IntAct
M3K1_HUMAN	MAP3K1	MINT
M3K3_HUMAN	MAP3K3	MINT
MEPCE_HUMAN	MEPCE	BioGRID
MK13_HUMAN	MAPK13	IntAct
MLP3A_HUMAN	MAP1LC3A	IntAct
MLP3B_HUMAN	MAP1LC3B	IntAct
MTR1A_HUMAN	MTNR1A	HPRD
MTR1B_HUMAN	MTNR1B	HPRD
MYOME_HUMAN	PDE4DIP	String
NDRG1_HUMAN	NDRG1	HPRD
NEMO_HUMAN	IKBKG	MINT
NFKB1_HUMAN	NFKB1	MINT
NFKB2_HUMAN	NFKB2	MINT
NHLC1_HUMAN	NHLRC1	BioGRID
O43318-2	MAP3K7	MINT
P53_HUMAN	TP53	MINT
PAWR_HUMAN	PAWR	HPRD
PAWR_RAT	Pawr	IntAct
PCSK7_HUMAN	PCSK7	HPRD
PDIP3_HUMAN	POLDIP3	String
PIAS1_MOUSE	Pias1	BioGRID
PRIO_HUMAN	PRNP	HPRD
PSME3_HUMAN	PSME3	BioGRID
Q00653-2	_	MINT
Q04206-2	RELA	MINT
Q14286_HUMAN	F8B	HPRD
Q2M4G9_MOUSE	Pias1	BioGRID
Q4LE70_HUMAN	APC	BioGRID
Q53YK7_HUMAN	PRNP	HPRD
Q569J5_HUMAN	SVIL	HPRD
Q59GL5_HUMAN	_	HPRD
Q5QT33_HUMAN	HLA-C	BioGRID
Q5RIP0_HUMAN	HLA-C	BioGRID
Q6FG91_HUMAN	GABARAPL2	IntAct
Q6IPR8_HUMAN	TRA@	HPRD
Q6IS15_HUMAN	EPM2A	BioGRID
Q6QEF7_HUMAN	BCAR1	BioGRID
Q6R739_HUMAN	HLA-Cw	HPRD
Q6ZPA8_HUMAN	_	BioGRID
Q8NC57_HUMAN	_	MINT
Q8NCY3_HUMAN	DKFZp434N1610	BioGRID
Q8TB90	TSHR	HPRD
Q95HC2_HUMAN	HLA-C	HPRD
Q9H3D4-1	_	IntAct
RAF1_HUMAN	RAF1	IntAct
RELB_HUMAN	RELB	MINT
RIPK1_HUMAN	RIPK1	MINT
RIPK3_HUMAN	RIPK3	MINT
RPC8_HUMAN	POLR3H	BioGRID
RUVB2_HUMAN	RUVBL2	BioGRID
SIL1_HUMAN	SIL1	HPRD
SIRT1_HUMAN	SIRT1	IntAct
SIRT3_HUMAN	SIRT3	IntAct
SPG20_HUMAN	SPG20	IntAct
SRBP2_HUMAN	SREBF2	BioGRID
SUMO4_HUMAN	SUMO4	HPRD
SVIL_BOVIN	SVIL	MINT
SVIL_HUMAN	SVIL	HPRD
T132A_HUMAN	TMEM132A	HPRD
TAB1_HUMAN	TAB1	MINT
TAB2_HUMAN	TAB2	MINT
TANK_HUMAN	TANK	MINT
TBK1_HUMAN	TBK1	MINT
TCRG1_HUMAN	TCERG1	HPRD
THYG_HUMAN	TG	HPRD
TMM62_HUMAN	TMEM62	IntAct
TNR1A_HUMAN	TNFRSF1A	MINT
TNR1B_HUMAN	TNFRSF1B	MINT
TRAF2_HUMAN	TRAF2	MINT
TRAF6_HUMAN	TRAF6	MINT
TSHR_HUMAN	TSHR	HPRD
UBQL4_HUMAN	UBQLN4	BioGRID
VWF_HUMAN	VWF	HPRD

Other Protein-Protein interaction resources

String database

View Microarray data

Temporarily unavailable

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