1. O. Rosok, F. Pedeutour, A. H. Ree and H. C. Aasheim (1999) Identification and characterization of TESK2, a novel member of the LIMK/TESK family of protein kinases, predominantly expressed in testis. Genomics 61(1): 44-54.
Abstract In this study we present the cDNA sequence of a novel putative protein kinase, denoted TESK2. The open reading frame of TESK2 encodes a putative 555-amino-acid protein, including a protein kinase consensus sequence in the N-terminal half. The protein kinase domain of TESK2 is structurally similar to the kinase domain of the protein serine/threonine kinase TESK1 (64% identity) and to those of the LIMK1 and LIMK2 kinases (42 and 39% identity, respectively). TESK2, together with TESK1, constitutes a second subgroup of the LIMK/TESK family of protein kinases, as revealed by phylogenetic analysis of the protein kinase domains. Chromosomal localization of human TESK2 was assigned to 1p32. Expression analysis of human TESK2 revealed a single mRNA species of 3.0 kb predominantly expressed in testis and prostate and low expression in most other tissues examined. Rat testicles expressed a single species of TESK2 mRNA of approximately 3.5 kb. However, the transcript was first detectable in rat testis after day 30 of postnatal development and was predominantly expressed in round spermatids. These observations suggest that TESK2 plays an important role in spermatogenesis. PMID: [10512679]
Figures for illustrating the function of this protein/gene
Function
Dual specificity protein kinase activity catalyzingautophosphorylation and phosphorylation of exogenous substrates onboth serine/threonine and tyrosine residues. Phosphorylatescofilin at 'Ser-3'. May play an important role in spermatogenesis.
CHAIN 1 571 Dual specificity testis-specific protein kinase 2. /FTId=PRO_0000086749. DOMAIN 58 313 Protein kinase. NP_BIND 64 72 ATP (By similarity). ACT_SITE 176 176 Proton acceptor (By similarity). BINDING 87 87 ATP (By similarity). MOD_RES 219 219 Phosphoserine; by autocatalysis (By similarity). VAR_SEQ 116 131 Missing (in isoform 2). /FTId=VSP_004930. VAR_SEQ 265 293 Missing (in isoform 3). /FTId=VSP_004931. VARIANT 11 11 G -> A (in a breast infiltrating ductal carcinoma sample; somatic mutation). /FTId=VAR_041214. CONFLICT 455 455 R -> C (in Ref. 6; AAH33085). Back to Top